Biocatalyst Engineering

We have construct protein engineering platforms for enhancement of the catalytic properties of the natural enzymes via molecular modification methods based on protein structure-activity relationship.

Amount of crystal structures have been determined in our lab up to now.

Different protein engineering methods can be applied to improve the catalytic efficiency, enantioselectivity or stabilization of enzymes. Directed evolution is a powerful tool in enzyme engineering and a series of techniques have been developed for the creation of diverse protein libraries. Error-prone PCR offers entirely random mutagenesis for a wide range of engineering goals. Through sequential rounds of random mutation and efficient high-throughput screening, error-prone PCR may uncover extraordinary mutations. Structure-based rational design has also become a very useful strategy for protein engineering, with smaller search libraries and thus less screening cost compared with traditional experimental methods.