题目: The Peace and War of Candida albicans in Oral Cavity
报告人: 四川大学口腔疾病研究国家重点实验室 任彪副教授
任彪，副教授，四川大学华西口腔医学院 口腔疾病研究国家重点实验室，硕士研究生导师。2012年毕业于中国科学院微生物研究所，2017赴哈佛医学院波士顿儿童医院研修。以口腔微生物资源、口腔微生态与疾病防治，微生物耐药及抗感染药物为主研方向，先后主持自然科学基金面上项目等国家级、省部级项目9项，发表SCI论文79篇，核心期刊11篇；主/参编中英文学术专著/教材8部，获授权国家发明专利8项，获四川省科技进步一等奖1项。长期担任Applied Microbiology and Biotechnology、International Journal of Oral Science，Archives of Oral Biology、Frotiers of Microbioloy、Journal of Dental Research、华西口腔医学杂志、国际口腔医学杂志等杂志期刊审稿人。
报告摘要：Candida albicans has emerged as a leading opportunistic pathogen in human microbiome, especially in immunocompromised population. The goal of our team is to understand the roles of C. albicans in oral cavity: 1. how C. albicans cooperates with oral bacteria (peace); 2. how C. albicans causes oral infections (war); and 3. how to treat C. albicans with new compounds or drug combinations (war). Here we introduced that the ergosterol biosynthesis pathway of C. albicans was essential for its interaction with oral bacteria and oral mucosal infection, and azole drugs can target at this pathway to block the interactions. Briefly, C. albicans can promote the cariogenic virulence ofActinomyces viscosus during the cariogenic pathogenicity in root caries. The ERG11 gene was critical for their interaction (peace with oral bacteria). Voriconazole can target at this gene to inhibit their interaction and reduce the cariogenic virulence (war against C. albicans). TheERG11 gene, together with ERG3, are also critical for oral mucosal infection of C. albicans through their regulations on the virulence factors, such as ALS1, SAP6 and HWP1 (war with host). Fortunately, only non-killing dosages of fluconazole, which targets at the ergosterol pathway, can block the oral mucosal infection both in vitro and in vivo. To directly treat C. albicans, we also identified many new compounds and combinations (war against C. albicans), including Chinese Traditional Medicine (TCM). We found that one the TCM can competitively inhibit the binding between mitochondrial complex I and its coenzyme flavin mononucleotide (FMN), then synergized with azoles to treat C. albicansinfection both in vitro and in vivo. In summary, the ergosterol biosynthesis pathway of C. albicans is essential for its homeostasis in oral cavity and it is also a good target for developing new antifungal compounds or combinations.