研究团队
王华磊 副教授
发布时间：2019-04-08 18:04:00
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王华磊 副教授 2001.9-2004.6 B.S., Ocean University of China 2004.9-2007.6 M.S., Ocean University of China 2007.7-2009.2 HangZhou EnzySource Co., Ltd., China 2009.3-2010.3 Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences 2010.4-2010.8 East China University of Science & Technology 2010.9-2013.11 Ph.D., East China University of Science & Technology 2013.12-2018.9 Lecturer, East China University of Science & Technology 2018.9- Associate Professor, East China University of Science & Technology
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王华磊 理学博士，华东理工大学生物工程学院副教授，硕士生导师。2004年和2007年于中国海洋大学分别获得学士和硕士学位，2013年华东理工大学生物化学与分子生物学专业博士毕业。主要研究方向为生物催化剂（酶）的高效开发、人工定制及其在手性药物中间体合成中的应用。2010年11月参与完成的“工业酶制剂的创制与应用关键技术”获上海市科技进步一等奖；2013年12月参与完成的“定向转化多元醇的细胞催化剂创制及其应用关键技术”获上海市技术发明一等奖。作为项目负责人主持国家自然科学基金面上项目、国家自然科学基金青年基金、中国博士后科学基金、华东理工大学基本科研业务费等4个纵向项目，并与永农生物科学有限公司、山东佰安瑞生物药业有限公司、枣庄市杰诺生物酶有限公司等合作开发横向项目3个，作为项目负责人承担总经费1300多万元，参与多项企业横向课题。在Applied and Environmental Microbiology, Microbial Cell Factories, Applied Microbiology and Biotechnology等学术期刊发表SCI论文二十余篇，申请中国发明专利8项，已授权4项。
Research Interests
- Discovery of novel enzymes and tailor-making biocatalysts
- Process development for the enzymatic production of chiral compounds
Selected Publications
1. HL Wang, WY Gao, HH Sun, LF Chen, LJ Zhang, XD Wang*, DZh Wei*. Protein engineering of nitrilase from Burkholderia cenocepacia J2315 for efficient and enantioselective production of (R)-o-chloromandelic acid. Applied and Environmental Microbiology, 2015, 81(24):8469-8477.
2. K Wu, HL Wang*, LF Chen, HY Fan, ZhQ Zhao, DZh Wei*. Practical two-step synthesis of enantiopure styrene oxide through an optimized chemoenzymatic approach. Applied Microbiology and Biotechnology. 2016, 100(20): 8757-8767.
3. WY Gao, K Wu, LF Chen, HY Fan, ZhQ Zhao, B Gao, HL Wang*, DZh Wei*. A novel esterase from a marine mud metagenomic library for biocatalytic synthesis of short-chain flavor esters. Microbial Cell Factories, 2016, 15:41..
4. LF Chen, YP Zhang, HY Fan, K Wu, JP Lin, HL Wang*, DZh Wei. Efficient bioreductive production of (R)-N-Boc-3-hydroxypiperidine by a carbonyl reductase. Catalysis Communications, 2017, 97:5-9.
5. HL Wang, HY Fan, HH Sun, L Zhao, DZh Wei*. Process development for production of (R)-(-)-mandelic acid by recombinant Escherichia coli cells harboring nitrilase from Burkholderia cenocepacia J2315. Organic Process Research & Development, 2015,19(12),2010-2016