本实验室关于工程谷氨酸棒杆菌以玉米芯渣物料生产γ-氨基丁酸的论文在Biochemical Engineering Journal发表。本文的第一作者单位为华东理工大学。徐迎迎第一作者，通讯作者为鲍杰，合作作者包括张斌（华东理工大学）。

Ying-Ying Xu , Bin Zhang*, Jie Bao*.Production of γ-aminobutyric acid using corncob residue as carbohydrate feedstock by engineered Corynebacterium glutamicum. Biochemical Engineering Journal, 2025, 215, 109629.

Abstract This study investigated Corynebacterium glutamicum for GABA production using corncob residue, a cheap carbon source, as carbohydrate feedstock instead of grains. Current fermentative production of γ-aminobutyric acid (GABA) mainly relies on starch and sugar feedstocks. Therefore, the use of cheap carbon sources can effectively reduce the production costs of GABA. This study validated the feasibility of an available non-food lignocellulose feedstock, corncob residue, for GABA fermentation. An engineered C. glutamicum was constructed by secretory expression of glutamate decarboxylase gadBmut and knock-out the GABA biodegradation gene. The fermentation parameters in shake flasks and bioreactors were optimized including the sugar concentration, nitrogen levels, cofactor levels and aeration rate, and fermentation mode. The fed-batch fermentation of the concentrated corncob residue hydrolysate achieved a record high GABA production of 93.15 g/L from lignocellulosic feedstock with a yield of 0.43 g/g glucose. The results provided technical support for the industrial production of GABA from low-cost lignocellulosic carbohydrates.

Keywords γ-Aminobutyric acid, corn cob residue, Corynebacterium glutamicum, glutamic acid