本实验室关于宛氏拟青霉适应性进化增强了对预处理木质纤维素原料中高浓度抑制剂的生物脱毒作用的论文在Bioresource Technology发表。本文的第一作者单位为华东理工大学。Agustian Chen与张斌为共同第一作者，通讯作者为鲍杰。

Agustian Chen¹, Bin Zhang¹, Jie Bao^*. Adaptive evolution of Paecilomyces variotii enhanced the biodetoxification of high-titer inhibitors in pretreated lignocellulosic feedstock. Bioresource Technology 411 (2024) 131351.

Abstract High inhibitor concentrations in lignocellulose feedstock negatively affect the degradation rate of bio-detoxification strains. This study designed two adaptive laboratory evolutions in solid substrate and liquid medium to boost the biodetoxification capacity of P. variotii to high titers of lignocellulose-derived inhibitors,resulting in two evolved strains AC70 and ZW70. The results showed that the evolutionary adaptation in liquid medium could better boost the acetic acid assimilation compared to that on solid substrate. Transcriptional analysis revealed that the evolved strains exhibited a significant upregulation of adh, acs, ach1, and ackA directly related to the initial steps of acetate and furan aldehydes metabolisms. ZW70 strain can effectively remove the high concentration inhibitors cocktail from the hydrolysates derived from pretreated wheat straw and furfural residues. The biodetoxified hydrolysates by ZW70 were successfully used for cellulose chiral L-lactic acid pro-duction with the titers of ~110 g/L, which were over 20 % higher than that detoxified by parental strain.

Keywords Paecilomyces variotii, lignocellulose, biorefinery, biodetoxification, adaptive laboratory evolution, L-lactic acid