Zhi-Jun Zhang

	Name: Zhi-Jun Zhang
	Title: Associate Professor
	Phone: 021-64250840
	Fax: 021-64250840
	Email: zjzhang@ecust.edu.cn

Education:

2002/9-2006/7 Inner Mongolia University of Science and Technology, Bioengineering, Bachelor

2006/9-2013/3 East China University of Science and Technology, Biochemical Engineering, PhD

2010/9-2012/9 University of Minnesota, Biochemistry and Biotechnology, Joint PhD student

Academic Positions：

2013/4-2015/4 East China University of Science and Technology, Postdoctoral

2015/4- East China University of Science and Technology, Lecturer

Research：

Discovery, design and molecular engineering of novel biocatalysts and their synthetic applications

Publication：

Journal papers：

1 Zhi-Jun Zhang, Hui-Lei Yu, Tadayuki Imanaka, Jian-He Xu*. Efficient production of (R)-()-mandelic acid by isopropanol-permeabilized recombinant E. coli cells expressing Alcaligenes sp. nitrilase. Biochem. Eng. J. 2015, 95: 7177

2. Zhi-Jun Zhang, Jiang Pan, Bao-Di Ma, Jian-He Xu*. Efficient biocatalytic synthesis of chiral chemicals. Adv. Biochem. Eng./Biotechnol. In press

3. Zhi-Jun Zhang, Jiang Pan, Chun-Xiu Li, Hui-Lei Yu, Gao-Wei Zheng, Xin Ju, Jian-He Xu*. Efficient production of (R)-()-mandelic acid using glutaraldehyde cross-linked Escherichia coli cells expressing Alcaligenes sp. nitrilase. Bioprocess Biosyst. Eng. 2014, 37: 12411248.

4.张志钧, 许国超, 许建和. 精细化学品绿色合成用工具酶的定向开发. 生物产业技术. 2013, 6: 12-19.

5. Zhi-Jun Zhang, Jiang Pan, Jun-Feng Liu, Jian-He Xu*, Yu-Cai He, You-Yan Liu. Significant enhancement of (R)-mandelic acid production by relieving substrate inhibition of recombinant nitrilase in toluene-water biphasic system. J. Biotechnol. 2011, 152:2429

6. Zhi-Jun Zhang, Jian-He Xu*, Yu-Cai He, Li-Ming Ouyang, You-Yan Liu. Cloning and biochemical properties of a highly thermostable and enantioselective nitrilase from Alcaligenes sp. and its potential for (R)-()-mandelic acid production. Bioprocess Biosyst Eng. 2011, 34:315322.

7. Zhi-Jun Zhang, Jian-He Xu*, Yu-Cai He, Li-Ming Ouyang, You-Yan Liu, Tadayuki Imanaka. Efficient production of (R)-()-mandelic acid with highly substrate/product tolerant and enantioselective recombinant Alcaligenes sp. nitrilase. Process Biochem. 2010, 45:887891

8. Bao-Di Ma, Xu-Dong Kong, Hui-Lei Yu*, Zhi-Jun Zhang, Shuai Dou, Yan-Peng Xu, Yan Ni, Jian-He Xu*. Increased catalyst productivity in α-hydroxy acids resolution by esterase mutation and substrate modification. ACS Catal. 2014, 4: 1026-1031.

9. Guo-Chao Xu, Hui-Lei Yu*, Zhi-Jun Zhang, Jian-He Xu*. Stereocomplementary bioreduction of β-ketonitrile without ethylated byproduct. Org. Lett. 2013, 15(21):54085411.

10.Gao-Wei Zheng, Xu-Yun Liu, Zhi-Jun Zhang, Ping Tian, Guo-Qiang Lin, Jian-He Xu*. Separation of enantiopure m-substituted 1-phenylethanols in high space-time yield using Bacillus subtilis esterase. RSC Adv. 2013, 3:2044620449.

11.Chen-Sheng Zhang, Zhi-Jun Zhang, Chun-Xiu Li, Hui-Lei Yu, Gao-Wei Zheng, Jian-He Xu*. Efficient production of (R)-o-chloromandelic acid by deracemization of o-chloromandelonitrile with a new nitrilase mined from Labrenzia aggregata. Appl. Microbiol. Biotechnol. 2012, 95:9199

12.Jun-Feng Liu, Zhi-Jun Zhang, Ai-Tao Li, Jiang Pan, Jian-He Xu*. Significantly enhanced production of recombinant nitrilase by optimization of culture conditions and glycerol feeding. Appl. Microbiol. Biotechnol. 2011, 89:665672

13.Yu-Cai He, Zhi-Jun Zhang, Jian-He Xu*, You-Yan Liu. Biocatalytic synthesis of (R)-()-mandelic acid from racemic mandelonitrile by cetyl-trimethylammoniumbromide-permeabilized cells of Alcaligenes faecalis ECU0401. J. Ind. Microbiol. Biotechnol. 2010, 37:741750

会议论文：

1.张志钧，许建和。重组大肠杆菌的通透、交联固定化及在合成手性扁桃酸的应用。第六届全国发酵工程学术研讨会，2014年6月18日-20日，无锡.

2.Zhi-Jun Zhang, Jian-He Xu*, Romas J. Kazlauskas*. Construction of ancestral enzymes for unnatural reactions. Enzyme Engineering XXII, September 22-26, 2013, Toyama, Japan.

3.Zhi-Jun Zhang, Bryan Jones, Antony Dean, Romas J. Kazlauskas*. Sequence-based reconstruction of ancestral enzymes to find catalytically promiscuous enzyme. 245th National Meeting of the American-Chemical-Society, April 7-11, 2013, New Orleans, USA.

4.Romas J. Kazlauskas*, Zhi-Jun Zhang, Bryan Jones, Jan von Langermann. Reconstruction of catalytically promiscuous ancestral enzymes as catalysts for unnatural reactions. 245th National Meeting of the American-Chemical-Society, April 7-11, 2013, New Orleans, USA.

5.张志钧、Romas J. Kazlauskas*、许建和*. 羟腈裂解酶的理性改造研究。长三角“先进生物制造-基础与应用”博士生论坛，2012，11月8日-10日，南京.

6.Zhi-Jun Zhang, Jian-He Xu, Romas J. Kazlauskas. Engineering an esterase into a nitroaldolase. Gordon Research Conference: Biocatalysis, July 8-13, 2012, Smithfield, USA.

7.张志钧，许建和。利用重组腈水解酶高效制备光学纯(R)-()-扁桃酸。第三届长三角研究生生物技术论坛。上海，2010年7月24-25日.

8.Zhi-Jun Zhang, Jian-He Xu. Markedly enhanced production of (R)-()-mandelic acid using recombinant nitrilase in a biphasic system. The 15th Symposium of Young Asian Biochemical Engineers' Community, Xiamen, Dec 18-20, 2009.

9.Zhi-Jun Zhang, Jian-He Xu, Yu-Cai He. Cloning and expression of a nitrilase gene from Alcaligenes sp. strain ECU0401 for production of (R)-(-)-mandelic acid. 12th, APCChE Congress, Dalian, August 4-5, 2008.

Monographs

Achievement：

Funding：

1.National Science Foundation (21406067) Data mining and structure-function relationship investigation of novel nitrile reductase 2015-2017 ¥250,000

2.China Postdoctoral Science Foundation (2014M560309) Genome mining and structure-function relationship investigation of nitrile reductase 2014-2015 ¥80,000

3.Fundamental Research Funds for the Central Universities (222201314016) Construction and rational design of the ancestral enzyme of halohydrin dehalogenase for the propranolol biosynthesis 2013-2015 ¥120,000

4.Company project Screening of microorganisms for tobacco fermentation 2014-2015 ¥450,000