庄英萍

1962年生，1984年毕业于华东化工学院生化工程专业后即留校任教，1997年获华东理工大学生化工程硕士学位，2006年获华东理工大学生物化工博士学位。现任华东理工大学生物工程学院分党委书记，国家生化工程技术研究中心（上海）副主任，上海生物制造产业技术研究院副院长，“863”生物和医药领域工业生物技术主题专家，中国微生物学会第十届理事会常务理事，生化过程模型化与控制专业委员会主任委员，中国化工学会生物化工专业委员会副主任委员。

长期来主要从事发酵过程的优化与放大研究，在国家生物反应器工程重点实验室和国家生化工程技术研究中心（上海）的工作中，在 “十一五”期间，完成了“973”项目“工业生物技术的过程科学基础研究--生化反应过程放大原理与方法”（课题编号：2007CB714303，课题负责人）“十二五”期间作为项目负责人，承担了上海市科委基地建设项目“上海生物过程工程专业服务平台再建设”（项目负责人，课题编号：11DZ2292700，13DZ2290500），并作为主要参加者，参加了“十一五”863重点项目“头孢菌素C发酵工业用菌种改造和代谢工程技术” 、“红霉素发酵工业用菌种改造与过程优化控制技术” 、“十二五”期间“863项目：生物过程关键技术及装备研发”、“973项目：工业生物过程高效转化与系统集成的科学基础研究”等重大项目。在长期研究中，本人所在的研究团队，围绕着工业生物过程的基础研究、关键共性技术研究和技术推广开展了大量工作，在多尺度发酵过程参数相关分析理论方法基础上，又形成了基于细胞生理特性和反应器流场特性相结合的工业生物过程优化与放大理论与方法及其相关的装备，并在红霉素、头孢菌素C、鸟苷、葡萄糖酸钠、阿维菌素等数十个品种的工业发酵产品中成功应用；本人在发酵过程的参数相关分析、发酵过程代谢调控和工业生物过程放大等方面形成了研究特色。近年来获得国家科技进步二等奖三项、上海市科技进步一等奖三项，中国产学研合作创新成果奖一项；发表论文150余篇，SCI收录90余篇，获授权发明专利25项。

获奖成果：

1、基于细胞生理与过程信息处理的工业发酵优化新技术，2011年国家科技进步二等奖，本人排名第三

2、基于参数相关的发酵过程生物反应器优化与放大技术，2002年国家科技进步二等奖，本人排名第三

3、新型食品添加剂呈味核苷酸二钠关键生产工艺，2004年获国家科技进步二等奖，本人排名第四

4、先进技术集成的红霉素生产新工艺 ，2008年上海市科技进步一等奖，本人排名第三

5、基于细胞生理与过程信息处理的工业发酵优化新技术，2014年中国产学研合作创新成果奖，本人排名第五

发表论文情况（SCI选录）：

1. Yong Chen, Zejiang Wang, Ju Chu*, Beili Xi, Yingping Zhuang. The glucose RQ-feedback control leading to improved erythromycin production by a recombinant strain Saccharopolyspora erythraea ZL1004 and its scale-up to 372-m(3) fermenter. Bioprocess & Biosystems Engineering. 2015, 38(1):105-112, DOI: 10.1007/s00449-014-1248-8

2. Hongzhong Lu，Chao Li，Wenjun Tang，Zejian Wang，Jianye Xia，Siliang Zhang，Yingping Zhuang，Ju Chu，Henk Noorman，Dependence of fungal characteristics on seed morphology and shear stress in bioreactor. Bioprocess & Biosystems Engineering. 2015, 38(5):917-928, DOI: 10.1007/s00449-014-1337-8

3. Fei Shi, Jun Tan, Ju Chu, Yonghong Wang, Yingping Zhuang, Siliang Zhang.  A qualitative and quantitative high-throughput assay for screening of gluconate high-yield strains by Aspergillus niger. Journal of Microbiologcal Methods. 109:134-139, DOI: 10.1016/ j.mimet.2014.12.004

4. Guan Wang, Ju Chu, Henk Noorman, Jianye Xia, Wenjun Tang, Yingping Zhuang, Siliang Zhang. Prelude to rational scale-up of penicillin production: a scale-down study. Applied Microbiology and Biotechnolog. 2014,   DOI:10.1007/s00253-013-5497-2

5. Minghui Xie, Jianye Xia*, Zhen Zhou, Ju Chu, Yingping Zhuang, Siliang Zhang, Flow Pattern, Mixing, Gas Hold-Up and Mass Transfer Coefficient of Triple-Impeller Configurations in Stirred Tank Bioreactors. Industrial & Engineering Chemistry Research. 2014, 53(14): 5941-5953, DOI: 10.1021/ie400831s

6. Minghui Xie, Jianye Xia*, Zhen Zhou,Zhou Guozhong, Ju Chu, Yingping Zhuang, Siliang Zhang, Henk Noorman,  Power consumption, local and average volumetric mass transfer coefficient in multiple-impeller stirred bioreactors for xanthan gum solutions. Chemical Engineering Science. 2014, 106:144-`56. DOI: 10.1016/j.ces.2013.10.032

7. Lan Li, Zejian Wang*, Xuejun Chen, Ju Chu, Yingping Zhuang, Siliang Zhang, Optimization of polyhydroxyalkanoates fermentations with on-line capacitance measurement. Bioresource Technology. 2014, 156:216-221. DOI: 10.1016/j.biortech.2014.01.042

8. Lei Deng, Bingyang Shi, Yingping Zhuang, Ju Chu, Xiaolin Shi, Siliang Zhang, Meijin Guo*, Performance and Mechanism of Neuroleukin in the Growth and Survival of Sertoli Cell-Induced Neurons in a Coculture System. Cell Transplantation. 2014, 23(3):  381-394, DOI: 10.3727/096368913X663578

9. Xiwei Tian,  Yonghong Wang*,  Ju Chu*,  Yingping Zhuang,  Siliang Zhang,. Oxygen transfer efficiency and environmental osmolarity response to neutralizing agents on L-lactic acid production efficiency by Lactobacillus paracasei. Process Biochemistry. 2014,49(12): 2049-2054, DOI: 10.1016/j.procbio.2014.06.014

10. Yongsheng Nie, Mingzhi Huang*, Junjie Lu, Jiangchao Qian, Weilu Lin, Ju Chu, Yingping Zhuang, Siliang Zhang.  Impacts of high beta-galactosidase expression on central metabolism of recombinant Pichia pastoris GS115 using glucose as sole carbon source via C-13 metabolic flux analysis. Journal of Biotechnology. 2014, 187:124-134, DOI: 10.1016/j.jbiotec.2014.07.011

11. Xiaoqing Hu, Ju Chu, Siliang Zhang, Yingping Zhuang,  Xin Wu,  Huaxin Chen,  Zhongyuan Lv, Zhongyi Yuan. An alkaline pH control strategy for methionine adenosyltransferase production in Pichia pastoris fermentation. Biotechnology & Bioprocess Engineering. 2014, 19(5):  900-907, DOI: 10.1007/s12257-014-0046-0

12. Xiwei Tian,  Yonghong Wang*,  Ju Chu*,  Yingping Zhuang,  Siliang Zhang,. L-Lactic acid production benefits from reduction of environmental osmotic stress through neutralizing agent combination. Bioprocess & Biosystems Engineering. 2014, 37(9): 1917-1923, DOI: 10.1007/s00449-014-1166-9

13. Taicheng Zhu, Haifeng Hang, Ju Chu, Yingping Zhuang, Siliang Zhang, Meijin Guo. Transcriptional investigation of the effect of mixed feeding to identify the main cellular stresses on recombinant Pichia pastoris. Journal of Industrial Microbiology & Biotechnology. 2013, 40(2) : 183-189   DOI: 10.1007/s10295-012-1225-7

14. Ju Chu, Jiangchao Qian, Yingping Zhuang, Siliang Zhang. Progress in the research of S-adenosyl-L-methionine production. Applied Microbiology Biotechnology. 2013, 97(1):41-49   DOI: 10.1007/s00253-012-4536-8

15. Yong Chen,Mingzhi Huang,Zejian Wang,Ju Chu,Yingping Zhuang,Siliang Zhang, Controlling the feed rate of glucose and propanol for the enhancement of erythromycin production and exploration of propanol metabolism fate by quantitative metabolic flux analysis. Bioprocess and Biosystems Engineering. 2013, 36(10): 1445-1453

16. Jun Tan,Ju Chu,Yuyou Hao,Yuanxin Guo,Yingping Zhuang,Siliang Zhang, High-Throughput System for Screening of Cephalosporin C High-Yield Strain by 48-Deep-Well Microtiter Plates. Applied Biochemistry and Biotechnology. 2013, 169(5): 1683-1695

17. Chao Li, Jianye Xia*, Ju Chu, Yonghong Wang*, Yingping Zhuang,; Siliang Zhang, CFD analysis of the turbulent flow in baffled shake flasks. Biochemical Eneineering Journal. 2013, 70:140-150

18. Yong Chen,  Zejian Wang,  Ju Chu, Yingping Zhuang,  Siliang Zhang,  Yu Xiaoguan, Significant decrease of broth viscosity and glucose consumption in erythromycin fermentation by dynamic regulation of ammonium sulfate and phosphate. Bioresource Technology. 2013,134:173-179. DOI: 10.1016/j.biortech.2013.02.023

19. Xiang Zou,Wanjun Li,Wei Zeng,Haifeng Hang,Ju Chu,Yingping Zhuang,Siliang Zhang, Biochemical Parameters of Saccharopolyspora Erythraea during Feeding Ammonium Sulphate in Erythromycin Biosynthesis Phase. Applied Biochemistry & Microbiology. 2013,49(2): 169-175   DOI: 10.1134/S0003683813020166

20. Xiang Zou, Wanjun Li, Wei Zeng, Hangfeng Hang, Ju Chu, Yingping Zhuang, Siliang Zhang. Biochemical Parameters of Saccharopolyspora Erythraea during Feeding Ammonium Sulphate in Erythromycin Biosynthesis Phase. Applied Biochemistry & Microbiology. 2013, 49(2):169-175. DOI: 10.1134/S0003683813020166

21. Xiaoqing Hu, Ju Chu. Siliang Zhang. Yingping Zhuang, Comparative performance of S-adenosyl-L-methionine biosynthesis and degradation in Pichia pastoris using different promoters and novel consumption inhibitors. 2013, Enzyme & Microbial Technology. 55:94-99. DOI: 10.1016/j.enzmictec.2013.09.003

22. Xiang Zou, Jian-ye Xia, Ju Chu, Ying-ping Zhuang, Si-liang Zhang, Real-time fluid dynamics investigation and physiological response for erythromycin fermentation scale-up from 50 L to 132 m(3) fermenter. Bioprocess & Biosystem Engineering, 2012, 35:789–800

23. Yue Hugh Guan, Remco N. A. M van den Heuvel, Ying-Ping Zhuang, Visualisation of J-type counter-current chromatography: A route to understand hydrodynamic phase distribution and retention. Journal of Chromatography A .  2012, 1239:10-21

24. Lan Yu, Nan Cao, Long Wang, Ciying Xiao, Meijin Guo, Ju Chu, Yingping Zhuang, Siliang Zhang, Oxytetracycline biosynthesis improvement in Streptomyces rimosus following duplication of minimal PKS genes. Enzyme & Microbial Technology, 2012, 50(6-7): 318-324

25. Ze-Jian Wang, Ping Wang, Yu-Wei Liu, Yi-Ming Zhang, Ju Chu, Ming-zhi Huang, Yingping Zhuang, Siliang Zhang, Metabolic flux analysis of the central carbon metabolism of the industrial vitamin B-12 producing strain Pseudomonas denitrificans using C-13-labeled glucose. Journal of the Taiwan Institute of  Chemical Engineers. 2012, 43(2): 181-187

26. Dan Wu, Ju Chu, Yu-You Hao, Yong-Hong Wang, Ying-Ping Zhuang, Si-Liang Zhang, Incomplete protein disulphide bond conformation and decreased protein expression result from high cell growth during heterologous protein expression in Pichia pastoris. Journal of Biotechnology. 2012,157(1):107-112

27. Yiming Yang, Jianye Xia, Jianhua Li ,Ju Chu, Liang Li, YonghongWang, Yingping Zhuang ,Siliang Zhang, A novel impeller configuration to improve fungal physiology performance and energy conservation for cephalosporin C production, Journal of Biotechnology. 2012, 161(3) : 250-256   DOI: 10.1016/j.jbiotec.2012.07.007  

28. Jun Tan, Ju Chu, Wenjuan Shi, Cheng Lin, Yuanxin Guo, Yingping Zhuang, Siliang Zhang, Tadayuki I manaka. High-throughput screening strategy used for enhanced production of pigment by Monascus purpureus D39-4. Food Science and Biotechnology. 2012, 21(6): 1603-1610   DOI: 10.1007/s10068-012-0213-z

29. Xiang Zou, Wan-jun Li, Wei Zeng, Ju Chu, Ying-ping Zhuang, Si-liang Zhang. An assessment of seed quality on erythromycin production by recombinant Saccharopolyspora erythraea strain . Bioresource Technology , 2011,102(3): 3360-3365  

30. Xu Li , Yonghong, Siliang, Ju Chu , Ming Zhang , Mingzhi Huang , Yingping Zhuang. Effects of light/dark cycle, mixing pattern and partial pressure of H-2 on biohydrogen production by Rhodobacter sphaeroides ZX-5. Bioresource Technology , 2011,102(2): 1142-1148

31. Taicheng Zhu , Meijin Guo , Yingping Zhuang , Ju Chu , Siliang Zhang. Understanding the effect of foreign gene dosage on the physiology of Pichia pastoris by transcriptional analysis of key genes. Applied Microbiology & Biotechnology. 2011, 89(4): 1127-1135

32. Xiulin Qin, Jiangchao Qian*, Gaofeng Yao, Yingping Zhuang, Siliang Zhang, and Ju Chu, GAP Promoter Library for Fine-Tuning of Gene Expression in Pichia pastoris. Applied and Environmental Microbiology, 2011, 77(11):3600-3608

33. Suwen Hu, Lei Deng, Huamao Wang, Yingping Zhuang, Ju Chu, Siliang Zhang, Zhonghai Li, Meijin Guo. Bioprocess development for the production of mouse-human chimeric anti- epidermal growth factor receptor vIII antibody C12 by suspension culture of recombinant Chinese hamster ovary cells. Cytotechnology. 2011,63(3): 247-258  

34. Xu Li, Huan Shi,, Yonghong Wang, , Siliang Zhang,  Ju Chu,  Ming Zhang,  Mingzhi Huang,  Yingping Zhuang, , Effects of vitamins (nicotinic acid, vitamin B(1) and biotin) on phototrophic hydrogen production by Rhodobacter sphaeroides ZX-5. International Journal of Hydrogen energy, .2011,36(16) : 9620-9625

35. Dan Wu, Ju Chu, Yu-You Hao, Yong-Hong Wang, Ying-Ping Zhuang, Si-Liang Zhang, High efficient production of recombinant human consensus interferon mutant in high cell density culture of Pichia pastoris using two phases methanol control. Process Biochemistry. 2011,46(8): 1663-1669

36. Zhenyu Tang, Ciying Xiao, Yingping Zhuang, Ju Chu, Siliang Zhang, Herron, Paul R.,  Hunter, Iain S., Meijin Guo, Improved oxytetracycline production in Streptomyces rimosus M4018 by metabolic engineering of the G6PDH gene in the pentose phosphate pathway, Enzyme & Microbial Technology, 2011, 49(1): 17-24

37. Jian-Guang Liang, Xiao-He Chu, , Zhi-Qiang Xiong, Ju Chu, Yong-Hong Wang, Ying-Ping Zhuang, Si-Liang Zhang, Oxygen uptake rate regulation during cell growth phase for improving avermectin B(1a) batch fermentation on a pilot scale (2 m(3)). World  Journal of Microbiology & Biotechnology, 2011, 27(11) : 2639-2644

38. Dan Wu, Ju Chu, Yu-You Hao, Yong-Hong Wang,  Ying-Ping Zhuang,  Si-Liang Zhang, Incomplete protein disulphide bond conformation and decreased protein expression result from high cell growth during heterologous protein expression in Pichia pastoris. J. Biotechnology, 2011. 157(1):107-112

39. Yifeng Lu ,Yonghong Wang, Guoqian Xu , Ju Chu, Yingping Zhuang , Siliang Zhang,Influence of High Solid Concentration on Enzymatic Hydrolysis and Fermentation of Steam-Exploded Corn Stover Biomass. Appl Biochem Biotechnol, 2010, 160:360–369

40. Yong-Hong Wang, Chun-Hai Wu, Ju Chu, Yu-You Hao, Ying-Ping Zhuang , Si-Liang Zhang, Regulation of branched-chain amino acid catabolism: glucose limitation enhances the component of isovalerylspiramycin for the bitespiramycin production. Bioprocess & Biosystem Engineering, 2010, 33:257–265

41. Dan Wu, Dong Ma, Yu-You Hao, Ju Chu, Yong-Hong, Wang，Ying-Ping Zhuang , Si-Liang Zhang, Incomplete formation of intramolecular disulfide bond triggers degradation and aggregation of human consensus interferon-α mutant by Pichia pastoris. Appl Microbiol Biotechnol , 2010, 85:1759–1767

42. Ze-Jian Wang, Hui-Yuan Wang , Yong-Liang Li, Ju Chu , Ming-Zhi Huang, Ying-Ping Zhuang, Si-Liang Zhang, Improved vitamin B12 production by step-wise reduction of oxygen uptake rate under dissolved oxygen limiting level during fermentation process. Bioresource Technology , 2010,101: 2845–2852

43. Wang, Yong, Zhuang, Ying-ping, Wang, Ping, Chu, Ju, Zhang, Si-liang. Regulation of meilingmycin in Streptomyces nanchangensis: Effect of ammonium ion, Korean Journal of  Chemical Engineering. 2010, 27(3): 910-914

44. Jianhua Li,Yiming Yang, Ju Chu,Mingzhi Huang, Lan Li,Xiaochun Zhang,Yonghong Wang , Yingping Zhuang ,Siliang Zhang ,Quantitative metabolic flux analysis revealed uneconomical utilization of ATP and NADPH in Acremonium chrysogenum fed with soybean oil, Bioprocess & Biosystems Engineering, 2010, 33(9) :1119-1129

45. Xiang Zou,Changfa Chen,Haifeng Hang,Ju Chu,Yingping Zhuang,Siliang Zhang , Response Surface Methodology for Optimization of the Erythromycin Production by Fed-Batch Fermentation Using an Inexpensive Biological Nitrogen Source, Chemical & Biochemical Engineering Quarterly. 2010, 24( 1): 95-100  

46. Ziqiang Xiong, Meijin Guo , Yuanxin Guo ,Ju Chu,Yingping Zhuang ,Siliang Zhang , RQ feedback control for simultaneous improvement of GSH yield and GSH content in Saccharomyces cerevisiae T65, Enzyme & Microbial Technology. 2010, 46( 7) : 598-602

47. Dan Wu, Ju Chu, Yu-You Hao, Yong-Hong Wang, Ying-Ping Zhuang, Si-Liang Zhang .Influence of Specific Growth Rate on Specific Productivity and Intermolecular disulfide bond of Recombinant Protein Produced by a Pichia pastoris Mut(+) Strain. Journal of Biotechnology, 2010, 150: S540-S540

48. Xiang Zou, Wei Zeng , Chang-fa Chen, Xia-chang Qi , Jiang-chao Qian, Ju Chu, Ying-ping Zhuang, Si-liang Zhang , Wan-jun Li, Fermentation Optimization and Industrialization of Recombinant Saccharopolyspora erythraea Strains for Improved Erythromycin A Production. Biotechnology & Bioprocess Engineering. 2010, 15(6): 959-968

49. Xiang Zou , Hai-feng Hang , Ju Chu, Ying-ping Zhuang, Si-liang Zhang, Oxygen uptake rate optimization with nitrogen regulation for erythromycin production and scale-up from 50 L to 372 m3 scale. Bioresource Technology. 2009, 100 (3) :1406–1412

50. Jin Zhou, Yong-Hong Wang, Ju Chu, Ling-Zhi Luo, Ying-Ping Zhuang, Si-Liang Zhang,Optimization of cellulase mixture for efficient hydrolysis of steam-exploded corn stover by statistically designed experiments.Bioresource Technology ,2009,100 :819–825

51. Zhi-Qiang Xiong, Mei-Jin Guo , Yuan-Xin Guo, Ju Chu, Ying-Ping Zhuang, Si-Liang Zhang，Efficient extraction of intracellular reduced glutathione from fermentation broth of  Saccharomyces cerevisiae by ethanol，Bioresource Technology，2009，100 :1011–1014

52. Zhen-Lin Li, Yong-Hong Wang , Ju Chu , Ying-Ping Zhuang and Si-Liang Zhang，Leucine improves the component of isovalerylspiramycins for the production of bitespiramycin，Bioprocess & Biosystem Engineering, 2009, 32: 641-647

53. Hui Hu, Jiangchao Qian, Ju, Yonghong, Yingping Zhuang , Siliang Zhang,Optimization of l-methionine feeding strategy for improving S-adenosyl-l-methionine production by methionine adenosyltransferase overexpressed Pichia pastoris，Applied Microbiology & Biotechnology, 2009, 83 (6): 1105-1114

54. Hui Hu, Jiangchao Qian, Ju, Yonghong, Yingping Zhuang , Siliang Zhang, DNA shuffling of methionine adenosyltransferase gene leads to improved S-adenosyl-l-methionine production in Pichia pastoris, Journal of Biotechnology, 2009, 141: 97–103

55. Xiang Zou, Hai-feng Hang , Ju Chu, Ying-ping Zhuang, Si-liang Zhang , Enhancement of erythromycin A production with feeding available nitrogen sources in erythromycin biosynthesis phase，Bioresource Technology,2009,100 (13) :3358-3365

56. Taicheng Zhu, Meijin Guo, Chen Sun, Jiangchao Qian , Yingping Zhuang, Ju Chu,Siliang Zhang ,A systematical investigation on the genetic stability of multi-copy Pichia pastoris strains，Biotechnology Letters, 2009, 31(5) : 679-684

57. Bin-Quan Gou, Ju Chu , Siliang Zhang, Yonghong Wang, Yingping Zhuang, Hua Huang, Zhen Li , Zhong-YiYuan ,Production of penicillin G amidase from Alcaligenes faecalis in a recombinant Escherichia coli，Journal of the Taiwan Institute of Chemical Engineerings, 2009, 40(2) : 233-236

58. Haifeng Hang, Xinhao Ye, Meijin Guo，Ju Chu, Yingping Zhuang, Ming Zhang, Siliang Zhang，A simple fermentation strategy for high-level production of recombinant phytase by Pichia pastoris using glucose as the growth substrate，Enzyme & Microbial Technology, 2009, 44 (4) :185-188

59. Jian-Ye Xia, Yong-Hong Wang, Si-Liang Zhang, Ning Chen, Peng Yin, Ying-Ping Zhuang, Ju Chu,Fluid dynamics investigation of variant impeller combinations by simulation and fermentation experiment, Biochemical Engineering Journal , 2009,43: 252–260

60. Yong Wang ,Ju Chu,Yingping Zhuang ,Yonghong Wang ,Jianye Xia,Siliang Zhang, Industrial bioprocess control and optimization in the context of systems biotechnology, Biotechnology Advances,2009, 27(6): 989-995    

61. Li X, Wang YH, Zhang SL, Chu J, Zhang M, Huang MZ, Zhuang YP , Enhancement of phototrophic hydrogen production by Rhodobacter sphaeroides ZX-5 using a novel strategy - shaking and extra-light supplementation approach，International Journal of Hydrogen Energy, 2009, 34(24) : 9677-9685  

62. Guo-Qian Xu, Ju Chu, Ying-Ping Zhuang, Yong-Hong Wang, Si-Liang Zhang, Effects of vitamins on the lactic acid biosynthesis of Lactobacillus paracasei NERCB 0401.Biochemical Engineering Journal. 2008, 38(2):189-197

63. Yun Chen, Wei Deng, Jiequn Wu, Jiangchao Qian, Ju Chu, Yingping Zhuang, Siliang Zhang and Wen Liu. Genetic Modulation of the Overexpression of Tailoring Genes eryK and eryG Leading to the Improvement of Erythromycin A Purity and Production in Fermentation of Saccharopolyspora erythraea. Applied and Environmental Microbiology. 2008, 74(6): 1820-1828.（4.004）

64. Jin Zhou, Ju Chu, Yong-Hong Wang, Si-Liang Zhang, Ying-Ping Zhuang,  Zhong-Yi Yuan, Purification and properties of Saccharomyces cerevisiae S-adenosylmethionine synthetase expressed in recombinant Pichia pastoris. World Journal of Microbiology & Biotechnology, 2008, 24(6):789–796

65. Zhou J, Wang YH, Chu J, Gou BQ, Zhuang YP, Zhang SL and Yuan ZY. Penicillin G acylase purification with the aid of high-throughput screening approach. Journal of the Chinese Institute of Chemical Engineers, 2008, 39: 195-202. （0.431）

66. Peng Yin, Yong-Hong Wang, Si-Liang Zhang, Ju Chu, Ying-Ping Zhuang, Mei-Long Wang, Jin Zhou， Isolation of soluble proteins from an industrial strain Streptomyces avermitilis in complex culture medium for two-dimensional gel electrophoresis. Journal of Microbiological Methods. 2008,73 (2) 105–110（2.153）

67. Feng  Lei, Guo Meijin, Zhang Shuxiang, Chu Ju, Zhuang Yingping, Zhang Siliang, Improvement in the suspension-culture production of recombinant adeno-associated virus-LacZ in  HEK-293 cells using polyethyleneimine –DNA complexes in combination with hypothermic treatment. Biotechnology and Applied Biochemistry, 2008, 50: 121-132

68. Ziqiang Xiong ,Meijin Guo ,Yuanxin Guo,Ju Chu ,Yingping Zhuang ,Siliang Zhang. Real-time viable-cell mass monitoring in high-cell-density fed-batch glutathione fermentation by Saccharomyces cerevisiae T65 in industrial complex medium. Journal of Bioscience and Bioengineering, 2008, 105(4): 409-413.（1.782）

69. Zhou J, Wang YH, Chu J, Zhuang YP, Zhang SL and Yin P. Identification and purification of the main components of cellulases from a mutant strain of Trichoderma viride T 100-14. Bioresource Technology, 2008, 99: 6826–6833.（3.103）

70. Kun-Tai Li, Dong-Hong Liu, Ju Chu, Yong-Hong Wang, Ying-Ping Zhuang, Si-Liang Zhang. An effective and simplified pH-stat control strategy for the industrial fermentation of vitamin B12 by Pseudomonas denitrificans. Bioprocess and Biosystems Engineering, 2008, 31（6）：606-610

71. Kun-Tai Li, Dong-Hong Liu, Ying-Ping Zhuang, Yong-Hong Wang, Ju Chu, Si-Liang Zhang. Influence of Zn2+, Co2+ and dimethylbenzimidazole on vitamin B12 biosynthesis by Pseudomonas denitrificans . World Journal of Microbiology and Biotechnology, 2008, 24（11）：2525-2530

72. Kun-Tai Li, Dong-Hong Liu, Yong-Liang Li, Ju Chu,Yong-Hong Wang, Ying-Ping Zhuang, Si-Liang Zhang. Improved large-scale production of vitamin B12 by Pseudomonas denitrificans with betaine feeding. Bioresource Technology, 2008, 99（17）：8516-8520

73. Xiao-Qing Hu, Ju Chu, Zhuo Zhang, Si-Liang Zhang, Ying-Ping Zhuang, Yong-Hong Wang, Mei-Jin Guo, Hua-Xin Chen, Zhong-Yi Yuan. Effects of different glycerol feeding strategies on S-adenosyl-L-methionine biosynthesis by PGAP-driven Pichia pastoris overexpressing methionine adenosyltransferase. Journal of Biotechnology ,2008, 137(1-4):44-49

74. Xiang Zou, Hai-feng Hang, Chang-fa Chen, Ju Chu, Ying-ping Zhuang, Si-liang Zhang，Application of oxygen uptake rate and response surface methodology for erythromycin production by Saccharopolyspora erythraea. J Ind Microbiol Biotechnol 2008, 35:1637–1642

75. Meijin Guo, Haifeng Hang, Taicheng Zhu, Yingpin Zhuang, Ju Chu, Siliang Zhang. Effect of glycosylation on biochemical characterization of recombinant phytase expressed in Pichia pastoris. Enzyme and Microbial Technology，2008，42（4）：340-345

76. Mei-jin Guo, Ying-ping Zhuang, Ju Chu, Si-liang Zhang, Ai-sheng Xiong, Ri-he Peng, and Quan-hong Yao.  Production and purification of a novel thermostable phytase by Pichia pastoris FPHY34. Process Biochemistry, 2007, 42（12）：1660-1665

77. Wang Yong, Wang Yiguang, Chu Ju, Zhuang Yingping, Zhang Lixin, Zhang Siliang,Improved production of erythromycin A by expression of a heterologous gene encoding S-adenosylmethionine synthetase ,Applied Microbiology and Biotechnology,2007, 75 (4): 837-842

78. Hu Xiaoqing, Chu Ju, Zhang Siliang, Zhuang Yingping, Wang Yonghong, Zhu Shan, Zhu Zhigang, Yuan Zhongyi, A novel feeding strategy during the production phase for enhancing the enzymatic synthesis of S-adenosyl-L-methionine by methylotrophic Pichia pastoris ,Enzyme and Microbial Technology, 2007, 40 (4): 669-674

79. Hao Yuyou, Chu Ju, Wang Yonghong, Zhuang Yingping, Zhang Siliang, The inhibition of aggregation of recombinant human consensus interferon-alpha mutant during Pichia pastoris fermentation , Applied Microbiology and Biotechnology ,2007,74 (3): 578-584

80. Siliang Zhang, Bangce Ye, Ju Chu, Yingping Zhuang, Meijin Guo, From multi-scale methodology to systems biology: to integrate strain improvement and fermentation optimization, Journal of Chemical Technology and Biotechnology, 2006,81 (5): 734-745

81. Jiangchao Qian, Xianpeng Cai, Ju Chu, Yingping Zhuang, Siliang Zhang, Nucleotide mutations in purA gene and pur operon promoter discovered in guanosine-and inosine-producing Bacillus subtilis strains. Biotechonlogy Letter, 2006, 28:937-941

82. Guo-qian Xu, Ju Chu, Yong-hong Wang, Ying-ping Zhuang, Si-liang Zhang and Hua-qiong Peng. Development of a continuous cell-recycle fermentation system for production of lactic acid by Lactobacillus paracasei. Proces .Biochemistry, 41(12): 2458-2463, 2006.

83. Zhuang Yingping, Ma Wenfeng, Guo Meijin, Ding Mansheng, Chu Ju, Zhang Siliang, High cell density and high expression of recombinant human ApoA-IMilano in Escherichia coli by twice temperature-shifted induction, Frontiers of Biology in China, 2006, 1(4):345-348

专著：

1. Siliang Zhang,Xuejun Cao,Ju Chu,Jiangchao Qian ,Yingping Zhuang ,Bioreactors and Bioseparation, Biotechnology in China: Chemicals, Energy & Envronment. Advances in Biochemical Engineering-Biotechnology.2010, 122: 105-150

2. Siliang Zhang, Ju Chu, Yingping Zhuang, A Multi-Scale Study of Industrial Fermentation Processes and Their Optimization, Adv Biochem Engin/Biotechnol , 87: 97–150, 2004

专利授权与申请：

1. 一种提高肌苷发酵过程糖苷转化率及产率的方法，发明申请号：200310122798.9，申请日期：2003-12-25,授权号： ZL 2003 1 0122798.9, 授权日期：2006-01-04, 申请人：储炬，陈双喜，张嗣良，庄英萍

2. 一种蚯蚓纤溶酶及其编码序列与用途，发明专利申请号：200510023241.9，申请日期：2005-01-12, 专利授权号ZL 2005 1 0023241.9，授权日：2007-12-19，申请人：储炬，黄文华，秦奕辉，庄英萍，张嗣良

3. 一种制备硫腺苷甲硫氨酸的改进方法，发明专利申请号：200510025183.3，申请日期：2005-04-19, 专利授权号 ZL 2005 1 0025183.3，授权日：2008-1-30，申请人： 张嗣良，储炬，胡晓清，朱志钢，朱闪，庄英萍，王永红

4. 一种重组复合α干扰素的制备方法，专利号申请号：200510028285.0 申请日期：2005-07-29, 专利授权号ZL 2005 1 0028285.0，授权日：2008-3-5，申请人：储炬，郝玉有，张嗣良，庄英萍，史琪琪，王永红，杭海峰，刘志敏

5. 一种新的腺苷甲硫氨酸的体外酶促转化方法, 申请号：200610024158.8, 申请日期：2006-02-27, 专利授权号ZL 2006 1 0024158.8，授权日：2008-1-23，申请人：庄英萍，武鑫，储炬，张嗣良，胡晓清，袁中一

6. 一种S-腺苷甲硫氨酸生产菌株的构建及大规模发酵，申请号：200610117828.0，申请日期：2006-11-1，授权专利号：ZL 2006 1 0117828.0，授权日期：2009-9-9，申请人：储炬，胡辉，王勇，张嗣良，庄英萍

7. 组成型大肠干菌基因工程菌的发酵工艺, 申请号：200610024465.6,申请日期：2006-03-08, 公开号：CN1821386A，申请人： 张嗣良，储炬，苟斌全，庄英萍，黄华，李震，王永红

8. 一种新的发酵生产乳酸的培养基配方，申请号：200610118858.3，申请日期：2006-11-29，公开号：CN1962851A，申请人：庄英萍，邱伙琴，徐国谦，储炬，张嗣良，王永红

9. 一种发酵生产S-腺苷甲硫氨酸的方法，申请号：200710036807.0，申请日期：2007-1-24，公开号CN101012470A，专利号：ZL200710036807.0，申请人：储炬，张卓，张嗣良，庄英萍，王永红，胡晓清，袁中一

10. 重组的降糖肽及其生产菌株的构建和培养方法，申请号：200710037133.6，申请日期：2007-2-5，公开号CN101029081A，申请人：庄英萍，王晖，储炬，张嗣良，钱江潮，王永红

11. 一种制备链霉菌全蛋白的方法，申请号：200710047439.X ，申请日期：2007-10-26日，公开号：CN101157721A，专利授权号：ZL200710047439.X，申请人：王永红, 银鹏, 张嗣良, 庄英萍, 储炬

12. 利用重组大肠杆菌高效生产丙酮酸氧化酶的培养基，申请号：200710047750.4，申请日期：2007-10-28，公开号：CN101182486A，授权号：200710047750.4，申请人：张嗣良，赵劼，王永红，储炬，庄英萍，袁中一

13. 利用氧化还原电位调控乳酸发酵，申请号200810034199.4，申请日期：2008年3月4日，公开号：CN101235397A，专利号：ZL200810034199.4，授权日：2010年12月8日，申请人：王永红，储炬，郑继岱，徐国谦，庄英萍，张嗣良

14. 基于氧化还原电位自动反馈调控厌氧发酵生产乙醇的方法，申请日期：2008年9月26日，申请号：200810200505.7，公开号：CN101358210A，专利号：ZL200810200505.7，申请人：杭海峰，王永红，王强，储炬，庄英萍，郑超，张嗣良

15. 一种重组人复合α干扰素（cIFN）异构体的分离方法，申请日期：2008年5月16日，申请号：200810037559.6，公开号：CN101580533A，专利号：ZL200810037559.6，申请人：庄英萍，杨琪，郝玉有，储炬，程杰，王维娜，符晓辉，张嗣良

16. 利用铵离子调控林可霉素发酵过程及提高林可霉素产素，申请日期2008年1月23日，申请号：200810032954.5，公开号：CN101220385.A，专利号：ZL200810032954.5，申请人：储炬，李啸，张嗣良，庄英萍，钟承赞，葛友群，杭海峰，王永红，郭美锦，夏建业，章付林，杨明

17. 一种快速酶解混合蛋白质的方法，申请号：200810207956.3，申请日期：2008年12月26日，授权号：ZL200810207956.3，授权日期：2011年11月9日，申请人：李友元，李雅萍，王永红，陈长华，储炬，庄英萍，张嗣良

18. 微孔板固定装置，申请号：200920068480.X，专利号：ZL2009200688480.X，申请日期：2009年3月5日，申请人：郝玉有，储炬，庄英萍，张嗣良（实用新型）

19. 一种微孔板盘，申请号：200920068504.1，专利号：ZL200920068504.1,申请日期：2009年3月6日，申请人：郝玉有，储炬，庄英萍，张嗣良（实用新型）

20. GAP启动子文库及其用途，申请号：200910053198.8，申请日期：2009年5月17日，公开号：CN101922047A，专利号：ZL200910053198.8，授权日：2012年12月26日，申请人：钱江潮，秦秀林，储炬，庄英萍，张嗣良，肖慈英

21. 基于生物信息的发酵过程工艺分析软件（简称发酵之星），登记号：2009SR027762，编号：软著登字第0154761号，著作权人：华东理工大学

22. 基于生物信息的发酵过程控制软件V1.0，登记号：2009SR027760，编号：软著登字第0154759号，著作权人：华东理工大学

23. 基于振荡-补光策略的新型光合生物制氢工艺，申请号：200910048227.2，公开号：CN101845460A，专利号：ZL 200910048227.2，授权日：2012年，申请日期：2009-3-26，申请人：王永红，张明，李旭，张嗣良，张波，储炬，庄英萍，鲍杰

23. 提高脱氮假单胞杆菌维生素B12产量的方法200810034948.3申请日期：2008-03-21，专利授权号：ZL 200810034948.3，专利授权日：2013-04-17，申请人：张嗣良；赵强；李昆太；刘东洪；庄英萍；储炬；王振国；李永亮

24. 基于氧消耗速率的维生素B12发酵生产控制工艺，专利申请号：200910196035.6，申请日期：2009-09-22，专利授权号：ZL200910196035.6，专利授权日：2013-04-17，申请人：张嗣良；王泽建；王慧媛；储炬；庄英萍

25. 发酵联动装置，申请号：201220588863.1，申请日期：2012年11月9日，专利授权号：ZL201220588863.1，授权日期：2013年5月8日，申请人：王泽建，张明，张剑坤，张嗣良，储炬，庄英萍，郭美锦，黄明志，夏建业，杭海峰（实用新型）

26. 微型全息式生物传感反应器系统，申请号：201010176549.8，申请日期：2010年5月19日，专利授权号：ZL201010176549.8，专利授权日：2013年6月26日，申请人：黄明志，王泽建，张一鸣，陈丽，刘玉伟，储炬，庄英萍，张嗣良

27. 一种可精确定时、定量并在位灭活的全自动快速取样设备，申请号：201310180350.0，申请日期：2013年5月15日，专利授权号：ZL201310180305.0，授权日期：2014年10月12日，申请人：黄明志，李敏超，王泽建，刘玉伟，储炬，庄英萍，张嗣良

28. 基于工业以太网技术的发本地过程数据通信软件，登记号：2013SR20247，授权日：2013年11月06日，开发完成日期：2013年05月27日，著作权人：华东理工大学

29. 一种头孢菌素C的发酵方法，申请号：201210429362.3，专利授权号：ZL201210429362.3，授权日期：2014年10月15日，申请日期：2012年10月31日，申请人：黎亮，张俊波，储炬，李树友，庄英萍，苗瑞春，张嗣良，陆俊