林金萍

发布时间：2018-09-04 18:09:00

林金萍

女，工学博士，生物化学与分子生物学专业硕士生导师，华东理工大学生物工程学院、生物反应器工程国家重点实验室副研究员。

主要从事生物催化与生物转化方向的研究。主持自然科学基金、“973”子课题等项目。发表SCI论文40余篇；申请发明专利 12项，其中授权7项；获得国家技术发明二等奖1项、上海市技术发明一等奖1项和上海市科技进步奖一等奖2项；获得国家新药临床批件2项、生产批文2项和新药证书1项，实现成果转让1项。

主要研究方向

1．新酶的筛选、发酵、纯化和功能表征；
2．酶的结构和功能解析；
3．生物催化合成手性化合物和生物基产品

代表性论文（*通讯作者）

1. Jian Deng, Kangling Chen, Zhiqiang Yao, Jinping Lin?, Dongzhi Wei?. Towards the computational design and engineering of enzymeenantioselectivity: A case study by a carbonyl reductase from Gluconobacter oxydans. Journal of biotechnology.2016,217:31-40

2. Yuanyuan Shi, Kefei Li, Jinping Lin*, Dongzhi Wei, Shengli Yang. Engineered expression vectors significantly enhanced the production of 2-keto-D-gluconic acid by Gluconobacter oxydans. Journal of Agricultural and Food Chemistry.2015, 63(22): 5492?5498

3. Xingxing Zhang, Baoqi Zhang, Jinping Lin*, Dongzhi Wei. Oxidation of ethylene glycol to glycolaldehyde using a highly selective alcohol dehydrogenase from Gluconobacter oxydans. Journal of Molecular Catalysis B: Enzymatic. 2015,112: 69–75

4. Rong Chen, Xu Liu, Jiale Wang, Jinping Lin?, Dongzhi Wei?. Cloning, expression, and characterization of an anti-Prelog stereospeci?c carbonyl reductase from Gluconobacter oxydans DSM2343. Enzyme and Microbial Technology. 2015, 70: 18–27

5. Jian Deng, Kangling Chen, Zhiqiang Yao, Jinping Lin?, Dongzhi Wei?. Efficient synthesis of optically active halogenated aryl alcohols at highsubstrate load using a recombinant carbonyl reductase fromGluconobacter oxydans. Journal of Molecular Catalysis B: Enzymatic.2015, 118:1–7

6. Xu Liu, Rong Chen, Zhongwei Yang, Jiale Wang, Jinping Lin*, Dongzhi Wei*. Characterization of a Putative Stereoselective Oxidoreductase from Gluconobacter oxydans and Its Application in Producing Ethyl (R)-4-Chloro-3-Hydroxybutanoate Ester. Molecular Biotechnology, 2014, 56:285–295

7. Daihuan Li, Jinping Lin*, Dongzhi Wei*. Improving Gluconobacter oxydans performance in the in situ removal of the inhibitor for asymmetric resolution of racemic 1-phenyl-1,2-ethanediol. Bioresource Technology, 2014, 159: 327–333

8. Lulu Shi, Kefei Li, Huan Zhang, Xu Liu, Jinping Lin?, Dongzhi Wei?. Identification of a novel promoter gHp0169 for gene expression in Gluconobacter oxydans. Journal of Biotechnology. 2014, 175:69-74

9. Xu Liu, Chao Wang, Lujia Zhang, Zhiqiang Yao, Dongbing Cui, Liang Wu, Jinping Lin,* Y. Adam Yuan, Dongzhi Wei. Structural and mutational studies on an aldo-keto reductase AKR5C3 from Gluconobacter oxydans. Protein science. 2014 ,23(11):1540-9

10. Xu Liu, Zuanning Yuan, Y. Adam Yuan, Jinping Lin*, Dongzhi Wei*. Biochemical and structural analysis of Gox2181, a new member of the SDR superfamily from Gluconobacter oxydans. Biochemical and Biophysical Research Communications. 2011,415: 410-415

11. Jian Wu, Minhua Li, Jinping Lin*, Dongzhi Wei*. Highly Selective Oxidation of Benzyl Alcohol Using Engineered Gluconobacter Oxydans in Biphasic System. Current Microbiology. 2011, 62:1123-1127

12. Jian Wu, Jiale Wang, Minhua Li, Jinping Lin*, Dongzhi Wei*. Optimization of immobilization for selective oxidation of benzyl alcohol by Gluconobacter oxydans using response surface methodology. Bioresource Technology. 2010, 101:8936-8941

13. Minghua Li, Jian Wu, Xu Liu, Jinping Lin*, Dongzhi Wei*, Hao Chen. Enchanced production of dihydroxyacetone from glycerol by overexpression of glycerol dehydrogenase in an alcohol dehydrogenase-deficient mutant of Gluconobacter oxydans. Bioresource Technology. 2010, 101:8294-8299

14. Minghua Li, Jian Wu, Jinping Lin*, Dongzhi Wei. Expression of Vitreoscilla Hemoglobin Enhances Cell Growth and Dihydroxyacetone Production in Gluconobacter oxydans. Current Microbiology. 2010,61:370-375

15. Minmin Chen, Jinping Lin*, Yushu Ma, Dongzhi Wei. Characterization of a Novel NADPH-Dependent Oxidoreductase From Gluconobacter oxydans. Molecular biotechnology. 2010, 46:176-181

16. Lin Zhang, Jinping Lin*, Yushu Ma, Dongzhi Wei, Ming Sun.Construction of a novel shuttle vector for use in Gluconobacter oxydans. Molecular biotechnology. 2010, 46：227-233

发明专利

1. 林金萍, 魏东芝，张行星。一种醇脱氢酶氧化二元伯醇的方法。ZL201310606518.5
2. 林金萍, 魏东芝，张行星。一种应用微生物细胞催化法制备羟基醛的方法。 ZL201310601537.9
3. 林金萍, 魏东芝，李克非。一种微生物细胞转化法生产2-酮基-D-葡萄糖酸的方法。ZL201310598049.7
4. 林金萍，魏东芝，吴建。一种同时分析微生物发酵液中甘油和二羟基丙酮的方法。ZL200910052444.9
5. 林金萍, 魏东芝, 李明华, 吴建。一种氧化葡萄糖酸杆菌（G.oxydans）的基因工程菌及其应用。ZL20091096972.1
6. 魏东芝，林金萍，高琼。一种微生物不对称拆分制备(S)-苯乙二醇的方法。ZL200810038899.0
7. 魏东芝，林金萍，张敏洁。一种酶法连续生产聚（ε-己内酯）的方法。ZL 200910045661.5
8. 林金萍, 魏东芝，石圆圆。一种适用于氧化葡萄糖酸杆菌的基因表达载体及其应用。CN 104513830A
9. 林金萍, 魏东芝，施露露。一种氧化葡萄糖酸杆菌启动子及其应用。CN 103740714A
10. 林金萍, 魏东芝，杨圆圆。一种硝基还原酶在芳香族硝基化合物降解还原中国的应用。CN 103642861A
11. 魏东芝，林金萍，魏国栋。一种应用静息细胞制备光学纯D-型β-羟基酸的方法。CN101280325A
12. 魏东芝，林金萍，许伟钦。从头孢克洛萘酚复合物中提纯头孢克洛的方法。CN 101314605

获奖

1.定向转化多元醇的生物催化剂创制及其应用关键技术。国家技术发明二等奖，2015
2. 定向转化多元醇的细胞催化剂创制及其应用关键技术。上海市技术发明奖，一等奖，2013
3.手性非天然氨基酸生物催化制备技术及其应用。上海市科技进步奖，一等奖，2012
4. 工业酶制剂的开发与应用关键技术。上海市科技进步奖，一等奖， 2010

联系方式

上海市梅陇路130号311信箱，邮编:200237
办公室：鲁华生物技术研究所（实验14楼东楼）402室
电话：021-64251923；传真：021-64250068
E-mail: jplin@ecust.edu.cn