Wensong Tan

Professor Wen-Song Tan

I. PERSONAL

Name: Wen-Song TAN

Address: State Key Laboratory of Bioreactor Engineering (SKLBE)

East China University of Science & Technology (ECUST)

130 Meilong Road Shanghai 200237 P. R. China

Telephone: 00 86 21 64250948

Fax: 00 86 21 64252250

E-mail wstan@ecust.edu.cn

Website http://acte.ecust.edu.cn/

Date of Birth: 20 February 1963

Place of Birth: Tongxiang, Zhejiang, P. R. China

Degree: Ph. D. in Chemical Engineering

Title: Professor, and Doctoral Supervisor in Biochemical Engineering, Chemical Engineering, and Bioengineering

Positions: Director of the Laboratory of Animal Cell and Tissue Engineering

Research Area: Bioprocess and Bioreactor Engineering

Animal Cell Culture Technology and Bioreactor Engineering

Stem Cell and Tissue Engineering

Member of Editors:

Journal of Bioscience and Bioengineering

Journal of East China University of Science and Technology (Natural Science Edition)

Journal Of Chemical Engineering of Chinese Universities (Co-Chairman of Editorial Committee)

After awarded Ph. D. in Chemical Engineering in July 1993, Professor Wen-Song Tan is always engaged in research and education in Chemical Engineering and Bioengineering at ECUST. His research area is in Bioprocess and Bioreactor Engineering, with emphasis on animal cell culture technology, stem cell bioengineering, and tissue engineering. A central theme of his research is applying engineering analysis to bioprocesses and bioreactors, biochemical and cellular systems, and integrating engineering and physiological insights into metabolic manipulation and generic regulation of cellular systems. During the past 20 years, he supervised and accomplished more than 20 projects from the State Government, Ministry of Education, and Shanghai Municipal Government. Based on these funds, considerable progress has been achieved in the research and developments of bioprocesses and bioreactors for animal cell cultures, human stem cell expansion and differentiation, and tissue engineering.

In recent years many accomplishments in the research work have been achieved. Those include (1) bioreactor design, scale-up, and intensifying operation, (2) bioprocess development and optimization for fed-batch and perfusion cultures, (3) serum-and protein-free medium development and application, (4) product quality control technology based on bioprocess design, (5) bioprocess development and optimization for viral vaccine production, (6) manufacturing applications of the mammalian cell culture bioprocesses for producing biologicals, (7) biotechnology and bioreactor systems for hematopoietic stem cell expansion, and (8) bioprocess and bioreactor systems for microtissue fabrication and macrotissue assembling. Most of these achievements are being industrialized in pilot and manufacturing scales in some Chinese leading pharmaceutical enterprises. So far, 22 invention patents have been authorized, and more than 250 papers have been published in domestic and foreign publications.

II. EDUCATION

Dates Institution Degree Field of Study

1980.9-1982.7 Hangzhou Secondary School of Chemical Technology Student Chem Machi & Equip

1988.9-1990.7 East China Institute of Chemical Technology (Now ECUST) MS Chemical Engineering

1990.9-1993.7 ECUST Ph. D. Chemical Engineering

Ph.D. Dissertation: The Studies on Hybridoma Cell Suspension Culture Technology and Airlift Bioreactors

III. EMPLOYMENT

Dates Organization Title

1982-1988 Tongxiang Pesticide Factory, Zhejiang Technician/Engineer

1993-1995 ECUST Research Associate

1995-1998 ECUST

Lab Animal Cell & Tissue Engineering Associate Professor

Director

1997 University of Bielefeld, Germany Visiting Professor, with Prof. Jurgen Lehmann

1996-2004 Institute of Biochemical Engineering, ECUST Director

1996-2009 Department of Science and Technology, ECUST Deputy Director

1998-2012 SKLBE Deputy Director

1998-present ECUST

Lab Animal Cell & Tissue Engineering Professor and Doctoral Supervisor

Director

2009-2015 Department of Engineering Education, ECUST Director

2012-present Hisun Institute of ECUST Dean

2014-present Zhangjiang Advanced Biotechnology Institute of ECUST Dean

IV. HONORS AND AWARDS

Award Name Organization Date

Shuguang Scholar Development Foundation of Shanghai Education 1996

Qimingxin Scholar Shanghai Municipal Science and Technology Commission 1998

Special Allowance Shanghai Municipal People’s Government 1999, 2000

LIDA Teacher Prize LIDA Foundation 2000

University Main Teacher Scholar Ministry of Education 2000

Outstanding Young Teacher Ministry of Education 2001

Leap-over-Century Excellent Scholar Ministry of Education 2002

V. CURRENT RESEARCH INTERESTS

1. Animal Cell Culture Process and Bioreactor Engineering

Fundamentals of mixing and mass transfer, cell-bubble and cell-turbulent eddy interactions in bioreactors.

Design, scale-up and scale-down, and intensifing operation of the animal cell culture bioprocess and bioreactors.

Optimization of the large scale and high density animal cell culture bioprocesses (fed-batch and perfusion) through metabolic and generic manipulation.

Fundamentals of the animal cell physiological state on viral infection and proliferation in viral vaccine production processes and bioreactors.

Design and development of serum-/protein-free and chemical-defined medium for animal cells.

2. Human Stem Cell Biotechnology

Influence of ex vivo culture environment on growth, biological function, and gene expression of the human stem cells.

Ex vivo cultivation, expansion, and directed differentiation induction technology and bioreactor engineering for human stem cells (hematopoietic stem cells, bone mesenchymal stem cells, iPS cells, and ES cells).

3. Tissue Engineering

Hydrodynamic and mass transfer phenomena in tissue engineering.

Interactions between biomaterials and stem cells in vitro.

Ex vivo construction technology and bioreactor engineering of the engineered human tissues.

VI. SELECTED PUBLICATIONS

2015：

[1]. Min Chen, Yi Zhang, Yan Zhou, Yan Zhang, Meidong Lang, Zhaoyang Ye, Wen-Song Tan (2015) Pendant small functional groups on poly(?-caprolactone) substrate modulate adhesion, proliferation and differentiation of human mesenchymal stem cells. Colloids and Surfaces. B, Biointerfaces, 134: 322-331.

[2]. Feiyue Xu, Lei Xu, Qi Wang, Yan Zhou, Zhaoyang Ye, Wen-Song Tan (2015) A three-dimensional dynamic coculture system enabling facile cell separation for chondrogenesis of mesenchymal stem cells. Biochemical Engineering Journal, 103: 68-76.

[3]. Jintao Liu, Xinning Chen, Li Fan, Xiancun Deng, H. Fai Poon, Wen-Song Tan, Xuping Liu (2015) Monitoring sialylation levels of Fc-fusion protein using size-exclusion chromatography as a process analytical technology tool. Biotechnology Letters, 37(7): 1371-1377.

[4]. Xintao Zhang, Hongping Tang, Ya-Ting Sun, Xuping Liu, Wen-Song Tan, Li Fan (2015) Elucidating the effects of arginine and lysine on a monoclonal antibody C-terminal lysine variation in CHO cell cultures. Applied Microbiology and Biotechnology, 99(16): 6643-6652.

[5]. Xintao Zhang, Ya-Ting Sun, Hongping Tang, Li Fan, Dongdong Hu, Jintao Liu, Xuping Liu, Wen-Song Tan (2015) Culture temperature modulates monoclonal antibody charge variation distribution in Chinese hamster ovary cell cultures. Biotechnology Letters, 37(11): 2151-2157.

[6]. Dongdong Hu, Yating Sun, Xuping Liu, Jintao Liu, Xintao Zhang, Liang Zhao, Haibin Wang, Wen-Song Tan, Li Fan (2015) Understanding the intracellular effects of yeast extract on the enhancement of Fc-fusion protein production in Chinese hamster ovary cell culture. Applied Microbiology and Biotechnology, 99(20): 8429-8440.

[7]. Ding Huang, Wenjuan Peng, Qian Ye, Xuping Liu, Liang Zhao, Li Fan, Kang Xia-Hou, Hanjing Jia, Jian Luo, Linting Zhou, Beibei Li, Shilei Wang, Wenting Xu, Ze Chen, Wen-Song Tan (2015) Serum-free suspension culture of MDCK cells for production of influenza H1N1 vaccines. PLOS ONE, 10(11): e0141686.

[8]. Yaya Liu, Weiyan Zhang, Xiancun Deng, Hong Fai Poon, Xuping Liu, Wen-Song Tan, Yan Zhou, Li Fan (2015) Chinese hamster ovary cell performance enhanced by a rational divide-and-conquer strategy for chemically defined medium development. Journal of Bioscience and Bioengineering, 120(6): 690-696.

[9]. Xuping Liu, Ding Huang, Wen-Song Tan, Jian Luo, Ze Chen (2015) Overcoming nutrient limitations for cell-based production of influenza vaccine. Human Vaccines & Immunotherapeutics, 11(7): 1685-1688.

[10]. Zheng Du, Ziyan Wang, Weiwei Zhang, Haibo Cai, Wen-Song Tan (2015) Stem cell factor is essential for preserving NOD/SCID reconstitution capacity of ex vivo expanded cord blood CD34(+) cells. Cell Proliferation, 48(3): 293-300.

2014：

[1]. Feiyue Xu, Lei Xu, Qi Wang, Zhaoyang Ye, Yan Zhou, Wen-Song Tan (2014) 3D dynamic culture of rabbit articular chondrocytes encapsulated in alginate gel beads using spinner flasks for cartilage tissue regeneration. BioMed Research International, 2014:539789.

[2]. Maiqin Chen, Min Zhou, Zhaoyang Ye, Yan Zhou, Wen-Song Tan (2014) Ectopic osteogenesis of macroscopic tissue constructs assembled from human mesenchymal stem cell-laden microcarriers through in vitro perfusion culture. PLoS One, 9(10): e109214.

[3]. Xiu Wang, Qingling Jiao, Songjie Zhang, Zhaoyang Ye, Yan Zhou, Wen-Song Tan (2014) Perfusion culture-induced template-assisted assembling of cell-laden microcarriers is a promising route for fabricating macrotissues. Biotechnology Journal, 9(11): 1425-34.

[4]. Qingchun Zhang, Ke Tan, Yan Zhang, Zhaoyang Ye, Wen-Song Tan, Meidong Lang (2014) In situ controlled release of rhBMP-2 in gelatin-coated 3D porous poly(ε-caprolactone) scaffolds for homogeneous bone tissue formation. Biomacromolecules, 15 (1): 84–94.

[5]. Houyong Luo, Maiqin Chen, Xiu Wang, Yang Mei, Zhaoyang Ye, Yan Zhou, Wen-Song Tan (2014) Fabrication of viable centimeter-sized 3D tissue constructs with microchannel conduits for improved tissue properties through assembly of cell-laden microbeads. Journal of Tissue Engineering and Regenerative Medicine, 8(6): 493-504.

[6]. Bin Lei, Xu Zhang, Minglong Zhu, Wensong Tan (2014) Effect of fluid shear stress on catalytic activity of biopalladium nanoparticles produced by Klebsiella Pneumoniae ECU-15 on Cr(VI) reduction reaction. Bioresources and Bioprocessing, 1: 28.

[7]. Ding Huang, Kang Xia-Hou, Xu-Ping Liu, Liang Zhao, Li Fan, Zhaoyang Ye, Wen-Song Tan, Jian Luo, Ze Chen (2014) Rational design of medium supplementation strategy for improved influenza viruses production based on analyzing nutritional requirements of MDCK Cells. Vaccine, 52(32) : 7091–7097.

2013：

[1]. Jianyun Ge, Haibo Cai, Qunliang Li, Zheng Du, Wen-Song Tan (2013) Effects of telomerase activity and apoptosis on ex vivo expansion of cord blood CD34+ cells. Cell Proliferation, 46:38-44.

[2]. Qiang Jing , Haibo Cai, Zheng Du , Zhaoyang Ye & Wen-song Tan (2013) Effects of agitation speed on the ex vivo expansion of cord blood hematopoietic stem/progenitor cells in stirred suspension culture. Artificial Cells, Nanomedicine, and Biotechnology, 41(2): 98–102.

[3]. Qingchun Zhang, Yun Jiang, Yan Zhang, Zhaoyang Ye, Wen-Song Tan, Meidong Lang (2013) Effect of porosity on long-term degradation of poly (ε-caprolactone) scaffolds and their cellular response. Polymer Degradation and Stability, 98(1): 209-18.

[4]. Qingchun Zhang, Houyong Luo, Yan Zhang, Yan Zhou, Zhaoyang Ye, Wen-Song Tan, Meidong Lang (2013) Fabrication of Three-dimensional Poly(ε-caprolactone) Scaffolds with Hierarchical Pore Structures for Tissue Engineering. Materials Science and Engineering: C, 33(4): 2094-2103.

[5]. Lei Xu, Qi Wang, Feiyue Xu, Zhaoyang Ye, Yan Zhou, Wen-Song Tan (2013) Mesenchymal Stem Cells Downregulate Articular Chondrocyte Differentiation in Non-contact Coculture Systems: Implications in Cartilage Tissue Regeneration. Stem Cells and Development, 22(11): 1657-1669.

[6]. Ya-Ting Sun, Liang Zhao, Zhao-Yang Ye, Li Fan, Xu-Ping Liu, and Wen-Song Tan (2013) An Investigation Into the Relationship Between Metabolic Responses and Energy Regulation in Antibody-Producing Cell. Journal of Microbiology and Biotechnology, 23(11): 1586–1597.

[7]. Ya-Ting Sun, Liang Zhao, Xu-Ping Liu, Dong-dong Hu, Li Fan, Wen-Song Tan (2013) Application of improved top-down approach in maximizing CHO cell mass and productivity in fed-batch culture. Journal of Chemical Technology and Biotechnology, 88(7): 1237-1247.

[8]. Ya-ting Sun, Liang Zhao, Zhaoyang Ye, Li Fan, Xu-ping Liu, Wen-Song Tan (2013) Development of a fed-batch cultivation for antibody-producing cells based on combined feeding strategy of glucose and galactose. Biochemical Engineering Journal, 81: 126-135.

[9]. Yan Xiao, Xu Zhang, Minglong Zhu, Wensong Tan (2013) Effect of the culture media optimization, pH and temperature on the biohydrogen production and the hydrogenase activities by Klebsiella pneumoniae ECU-15. Bioresource Technology, 137(6): 9-17.

[10]. Hongxing Niu, Zakaria Amribt, Patrick Fickers, Wensong Tan, Philippe Bogaerts (2013) Metabolic pathway analysis and reduction for mammalian cell cultures—Towards macroscopic modeling. Chemical Engineering Science, 102: 461-473.