Qiyao Wang

Qiyao Wang, PhD

Prof. of School of Biotechnology,

State Key Laboratory of Bioreactor Engineering,

East China University of Science and Technology

CONTACT INFORMATION

P.O. 431#, State Key Laboratory of Bioreactor Engineering,

School of Biotechnology,

East China University of Science and Technology (ECUST),

Meilong Rd. 130, Xuhui District, Shanghai, 200237, P. R. China

Email: oaiwqiyao@ecust.edu.cn

Phone: +86-21-64253306

Mobile: +86-13564644353

Fax: +86-21-64253306

WORK EXPERIENCES AND EDUCATION

RESEARCH INTERESTS

I was interested in using systems biology strategies to investigate the pathogenesis of bacterial pathogens, such as Vibrio alginolyticus and Edwardsiella tarda causing huge economic losses towards marine aquaculture industry in China. Biochemistry isolation, Transposon-based mutant library, Tn-based defined library as well as genomics and proteomics were carried out to define the virulence factors. Further their regulation mechanisms (network) were investigated in the light of the host and environmental-adaptation processes. The key regulation elements or virulence factors will be used to provide the molecule elements for design of novel vaccines.

UNDERWAY PROJECTS:

1. Investigation of type III and IV secretion systems (TTSS and T6SS) as well as their regulation in Edwardsiella tarda.

2. Construction of a defined mutant library for Edwardsiella tarda.

3. Dissection of the regulon of two-component system EsrAB in Edwardsiella tarda using RNA-seq and ChIP-seq technologies.

TEACHING

1. Bioinformatics, 32 h/semester, Biotechnology undergraduate students.

2. Micbrobial cellular signaling and transduction, 32 h/semester, PhD students.

PEER REVIEWED PUBLICATIONS (* corresponding author)

1.       Qiyao Wang*, Yves A. Millet, Michael C. Chao, Jumpei Sasabe, Brigid M. Davis  and Matthew K. Waldor*. A genome wide screen for factors controlling Vibrio cholerae virulence gene expression reveals that the phosphoenolpyruvate phosphotransferase system (PTS) modulates ToxT expression. Infect Immun, accepted

2.       Shuai Shao, Qiliang Lai, Qin Liu, Haizhen Wu, Jingfan Xiao, Zongze Sao, Qiyao Wang*, Yuanxing Zhang. Phylogenomics characterization of a highly virulent Edwardsiella strain ET080813T encoding two distinct T3SS and three T6SS gene clusters: Propose a novel species as Edwardsiella anguillarum sp. nov. Systematic and Applied Microbiology, 2015, 38(1): 36-47

3.       Weizheng Yang, Lixia Wang, Lingzhi Zhang, Jiangbo Qu, Qiyao Wang*, Yuanxing Zhang. An invasive and low virulent Edwardsiella tarda esrB mutant promising as live attenuated vaccine in aquaculture. Applied Microbiology and Biotechnology, 2015, 99(4): 1765-1777

4.       Yamin Wang, Weizheng Yang, Qiyao Wang*, Jiangbo Qu, Yuanxing Zhang. Presenting a foreign antigen on live attenuated Edwardsiella tarda using twin-arginine translocation signal peptide as a multivalent vaccine. Journal of Biotechnology, 2013, 168(4): 710-717

5.       Minjun Yang, Qiyao Wang*, Haizhen Wu, Jingfan Xiao, Qin Liu, Yuanxing Zhang. Phylogenetic investigation of Edwardsiella tarda with multilocus sequence typing (MLST) and pulsed field gel electrophoresis typing (PFGE) methods. Aquaculture, 2013, 410-411:79-85.

6.       Yamin Wang, Weizheng Yang, Qiyao Wang*, Yuanxing Zhang. Functional characterization of Edwardsiella tarda twin-arginine translocation system and its potential use as a biological containment for live attenuated vaccine in marine fish vaccinology. Applied Microbiology and Biotechnology, 2013, 97(8): 3543-3557

7.       Yamin Wang, Qiyao Wang*, Minjun Yang, Yuanxing Zhang. Proteomic analysis of a twin-arginine translocation deficient mutant unravel its functions involved in stress adaptation and virulence in fish pathogen Edwardsiella tarda. FEMS Microbiology Letters, 2013, 343:145-155.

8.       Yuanzhi Lv, Kaiyu Yin, Shuai Shao, Qiyao Wang*, Yuanxing Zhang. Comparative proteomic analysis reveals new components of the PhoP regulon and highlights a role for PhoP in the regulation of genes encoding the F1F0 ATP synthase in Edwardsiella tarda, Microbiology-SGM, 2013, 159: 1340-1351

9.       Jingfan Xiao, Tao Chen, Bin Liu, Weizheng Yang, Qiyao Wang*, Jiangbo Qu, Yuanxing Zhang. Edwardsiella tarda mutant disrupted in type III secretion system and chorismic acid synthesis and cured of a plasmid as a live attenuated vaccine in turbot. Fish & Shellfish Immunology, 2013, 35(3): 632-641

10.   Minjun Yang, Jingfan Xiao, Haizhen Wu, Yuanzhi Lv, Huajun Zheng, Qin Liu, Yuanxing Zhang, Qiyao Wang*. Edwardsiella comparative phylogenomics reveal the new intra/inter-species taxonomic relationships, virulence evolution and niche adaptation mechanisms. PLOS ONE. 2012, 7(5): e36987

11.   Dahai Yang, Qin Liu*, Minjun Yang, Haizhen Wu, Qiyao Wang, Jingfan Xiao, Yuanxing Zhang*. RNA-seq liver transcriptome analysis reveals an activated MHC-I pathway and an inhibited MHC-II pathway at the early stage of vaccine immunization in zebrafish. BMC Genomics, 2012, 13:319

12.   Meng Zhang, Haizhen Wu*, Xiaoyong Li, Minjun Yang, Tao Chen, Qiyao Wang, Qin Liu, Yuanxing Zhang. Edwardsiella tarda flagellar protein FlgD: A protective immunogen against edwardsiellosis. Vaccine, 2012, 30:3849-3856

13.   Zhihui Zhang, Haizhen Wu*, Jingfan Xiao, Qiyao Wang, Qin Liu, Yuanxing Zhang. Immune responses of zebrafish (Danio rerio) induced by bath-vaccination with a live attenuated Vibrio anguillarum vaccine candidate. Fish & Shellfish Immunology, 2012, 33:36-41

14.   Lili Sheng, Qiyao Wang*, Qin Liu, Yuanxing Zhang. Connection between Type VI secretion system, quorum sensing, and alternative sigma factor in fish pathogen Vibrio alginolyticus. Archives of Microbiology, 2012, 194:379-390

15.   Yuanzhi Lv, Jingfan Xiao, Qin Liu, Haizhen Wu, Yuanxing Zhang, Qiyao Wang*. Systematic mutation analysis of two-component signal transduction systems reveals EsrA-EsrB and PhoP-PhoQ as the major virulence regulators in Edwardsiella tarda. Veterinary Microbiology. 2012, 157:190-199

16.   Jingfan Xiao, Tao Chen, Qiyao Wang*, Yuanxing Zhang. Comparative analysis of the roles of catalases KatB and KatG in the physiological fitness and pathogenesis of fish pathogen Edwardsiella tarda. Letters in Applied Microbiology. 2012, 54:425-432

17.   Jingfan Xiao, Xin Wang, Tao Chen, Yuanxing Zhang, Qiyao Wang*. Identification of qseEGF genetic locus and its roles in regulating hemolytic activity and invasion in fish pathogen Edwardsiella tarda. Letters in Applied Microbiology, 2012, 55:91-98

18.   Huan Liu, Dan Gu, Lili Sheng, Qiyao Wang*, Yuanxing Zhang. Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Letters in Applied Microbiology, 2012, 55:73-81

19.   Yuanzhi Lv, Junyuan Zheng, Minjun Yang, Qiyao Wang*, Yuanxing Zhang. An Edwardsiella tarda mutant lacking UDP-glucose dehydrogenase shows pleiotropic phenotypes, attenuated virulence, and potential as a vaccine candidate. Veterinary Microbiology, 2012, 160(3-4): 506-512.

20.   Lili Sheng, Yuanzhi Lv, Qin Liu, Qiyao Wang*, Yuanxing Zhang. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA, Veterinary Microbiology, 2012, 162(2-4):652-662

21.   Huan Liu, Dan Gu, Xiaodan Cao, Qin Liu, Qiyao Wang*, Yuanxing Zhang*. Characterization of a new quorum sensing regulator luxT and its roles in the extracellular protease production, motility and virulence in fish pathogen Vibrio alginolyticus. Archives of Microbiology. 2012, 194:439-452

22.   Xin Wang, Qiyao Wang*, Minjun Yang, Jingfan Xiao, Qin Liu, Haizhen Wu, and Yuanxing Zhang*. QseBC controls flagellar motility, fimbrial hemagglutination and intracellular virulence in fish pathogen Edwardsiella tarda. Fish & Shellfish Immunology. 2011, 30: 944-953

23.   Huan Liu, Qiyao Wang*, Qin Liu, Xiaodan Cao, Cunbin Shi, Yuanxing Zhang*. Roles of Hfq in the stress adaptation and virulence in fish pathogen Vibrio alginolyticus and its potential application as a target for live attenuated vaccine, Applied Microbiology and Biotechnology, 2011, 91:353–364

24.   Jingfan Xiao, Tao Chen, Qiyao Wang*, Qin Liu, Xin Wang, Yuanzhi Lv, Haizhen Wu, and Yuanxing Zhang*. Search for live attenuated vaccine candidate against edwardsiellosis by mutating virulence-related genes of fish pathogen Edwardsiella tarda. Letters in Applied Microbiology. 2011, 53:430-437

25.   Yamin Wang, Qiyao Wang*, Jingfan Xiao, Qin Liu, and Yuanxing Zhang. Genetic Relationships of Edwardsiella strains isolated in China aquaculture revealed by rep-PCR genomic fingerprinting and investigation of Edwardsiella virulence genes. Journal of Applied Microbiology, 2011, 111:1337-1348

26.   Lingyu Guan, Wei Mu, Jonathan Champeimont, Qiyao Wang, Haizhen Wu, Jingfan Xiao, Werner Lubitz, Yuanxing Zhang, Qin Liu*. Iron-regulated lysis of recombinant Escherichia coli in host releases protective antigen and confers biological containment. Infection and Immunity, 2011, 79:2608-2618

27.   Yan Zhao, Qin Liu*, Xiuhua Wang, Lingyun Zhou, Qiyao Wang, Yuanxing Zhang*. Surface display of Aeromonas hydrophila GAPDH in attenuated Vibrio anguillarum to develop a novel multivalent vector vaccine. Marine Biotechnology, 2011, 13:963-970

28.   Xiaodan Cao, Qiyao Wang, Qin Liu*, Haopeng Rui, Huan Liu, Yuanxing Zhang*. Identification of a luxO-regulated extracellular protein Pep and its roles in motility in Vibrio alginolyticus. Microbial Pathogenesis, 2011, 50: 123-131

29.   Xin Wang, Qiyao Wang, Jingfan Xiao, Qin Liu*, Haizhen Wu, Yuanxing Zhang*. Hemolysin EthA in Edwardsiella tarda is essential for fish invasion in vivo and in vitro and regulated by two-component system EsrA-EsrB and nucleoid protein HhaEt. Fish & Shellfish Immunology. 2010, 29:1082-1091

30.   Jingfan Xiao, Qiyao Wang*, Qin Liu, Lili Xu, Xin Wang, Haizhen Wu, and Yuanxing Zhang* Characterization of Edwardsiella tarda rpoS: effect on serum resistance, chondroitinase activity, biofilm formation, and autoinducer synthetases expression, Applied Microbiology and Biotechnology, 2009, 83:151-160

31.   Qiyao Wang, Minjun Yang, Jingfan Xiao, Haizhen Wu, Xin Wang, Yuanzhi Lv, Lili Xu, Huajun Zheng, Shengyue Wang, Guoping Zhao, Qin Liu, Yuanxing Zhang. Genome sequence of the versatile fish pathogen Edwardsiella tarda provides insights into its adaptation to broad host ranges and intracellular niches. PLoS ONE, 2009, Oct. 17, 4(10): e7646

32.   Xin Wang, Qiyao Wang*, Jingfan Xiao, Qin Liu, Haizhen Wu, Lili Xu, Yuanxing Zhang*. Edwardsiella tarda T6SS component evpP is regulated by esrB and iron, and plays essential roles in the invasion of fish. Fish & Shellfish Immunology, 2009, 27: 469-477

33.   Qiyao Wang, Zhao Yang, Qin Liu, Yuanxing Zhang. Surface-exposed expression of Edwardsiella tarda EseB in live attenuated Vibrio anguillarum based on novel surface display systems. Aquaculture research, 2009, 40:1459-1467

34.   Zhao Yang, Qin Liu*, Qiyao Wang, Yuanxing Zhang*. Novel Bacterial Surface Display systems based on outer membrane anchoring elements from the marine bacterium Vibrio anguillarum. Applied and Environmental Microbiology. 2008, 74, 4359-4365

35.   Qiyao Wang, Qin Liu, Xiaodan Cao, Minjun Yang, Yuanxing Zhang. Characterization of two TonB systems in marine fish pathogen Vibrio alginolyticus: their roles in iron utilization and virulence. Archives of Microbiology, 2008, 190:595-603

36.   Qiyao Wang, Qin Liu, Yue Ma, Haopeng Rui, Yuanxing Zhang. LuxO controls extracellular protease, hemolytic activities and siderophore production in fish pathogen Vibrio alginolyticus, Journal of Applied Microbiology, 2007, 103:1525-1534

37.   Qiyao Wang, Qin Liu, Yue Ma, Lingyun Zhou, Yuanxing Zhang. Isolation, sequencing and characterization of cluster genes involved in the biosynthesis and transportation of the siderophore of marine fish pathogen Vibrio alginolyticus, Archives of Microbiology, 2007, 188:433-439

CONFERENCE PAPERS/TALKS

1.       Qiyao Wang. FIPHA: Fitness pattern based heuristic approach to unravel novel virulence regulators and vaccine targets in zoonotic pathogen Edwardsiella bacteria, FORC International symposium, June 2, 2015, Seoul National University, Seoul, Korea (invited speaker)

2.       Qiyao Wang. FIPHA: Fitness pattern based heuristic approach to unravel novel virulence regulators and vaccine targets in zoonotic pathogen Edwardsiella bacteria, KoSFoST 2015, June 4, 2015, Busan, Korea (invited speaker)

3.       Qiyao Wang. A genome wide screen for factors controlling Vibrio cholerae virulence gene expression reveals that the phosphoenolpyruvate phosphotransferase system (PTS) modulates ToxT expression. Oct. 31, 2014. Havard Medical School/Brigham & Women’s Hospital. Pathogen and Infectious Diseases series Symposium. Boston (oral presentation)

4.       Qiyao Wang. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. China and Denmark Workshop on Bacterial QS Signals and QS Interference, Sept. 26-27, 2012, Beijing, China (invited speaker).

5.       Qiyao Wang. Comparative genomics of fish pathogen Edwardsiella tarda. Australia Society of Microbiology 2012 Year Meeting, Jul. 1-4, Brisbane (poster and abstract).

6.       Qiyao Wang. Edwardsiella tarda: From genomics to comparative phylogenomics. National conference on microbiology genomics, Nov. 26-28, 2011, Xiamen, China (invited speaker).

7.       Qiyao Wang. Hemolysin EthA in Edwardsiella tarda is essential for fish invasion in vivo and in vitro and regulated by two-component system EsrA-EsrB and nucleoid protein HhaEt. Host-Pathogen Interaction Conference, Sept. 4-7, 2010, Amsterdam (poster and abstract).

8.       Qiyao Wang. Quorum sensing regulates flagella biogenesis and virulence in fish pathogen Vibrio alginolyticus. Vibrio_2007, Nov. 28-Dec. 01, 2007, Institut Pasteur, Paris (poster and abstract).

COLLABORATORS:

1.       Dr. Matthew K. Waldor, Edward H. Kass chair professor, Harvard Medical School/HHMI, USA

2.       Dr. Ka Yin Leung, professor and Dean, Department of Biology, Trinity Western University. Canada

3.       Dr. Minjun Yang, Shanghai - MOST Key Laboratory of Health and Disease Genomics,    Chinese National Human Genome Center at Shanghai, Shanghai, China

4.       Dr. Xiaohui Zhou, Department of Pathobiology and Veterinary Science, University of Connecticut, USA

5.       Dr. David Reverter, professor, Institut de Biotecnologia i Biomedicina, Dept. de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Spain