Bin-Cheng Yin

Dr. Bin-Cheng Yin

Office phone: +86-21-64253832

Email: binchengyin@ecust.edu.cn

Address: Lab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai, Meilong Road 130, 200237, China  

Our group mainly focuses on bioanalytical technology for basic studies and applications. We apply fundamental principles of molecular biology, chemistry, and nanomaterial to develop innovative nano-bio probes, biosensing mechanisms, and devices to advance a variety of areas including biomedical diagnosis, environmental monitoring, and food security.

Currently, we strive to make significant contributions in the following research topics.

      Novel signal amplification strategy

We rationally design various enzyme-assisted target recycling and regeneration strategies to improve the sensitivity and stability of biosensors. Particularly, we develop novel isothermal biosensors for point-of-care diagnosis.

      Bio-inspired assembly and nanotechnology

In molecular engineering, we take advantage of functional biomolecules (such as DNA, peptide) as the building block to develop novel nano-bio probe for advanced bioanalytical applications in biosensors for detecting biomarkers such as circulating nucleic acids.

? Shanghai Graduate Outstanding Achievement (Thesis), 2013

? Meiji Dairy Science Award, 2009

? National Conference Ph.D. (Applied Chemistry) Outstanding Paper Award, 2009

? Shanghai Technological Invention Award (2nd Rank), 2008

1.        Jin-Liang Ma, Bin-Cheng Yin,* Huynh-Nhu Le, and Bang-Ce Ye*. Label-Free Detection of Sequence-Specific DNA based on Fluorescent Silver Nanoclusters-Assisted Surface Plasmon Enhanced Energy Transfer. ACS Applied Materials & Interfaces 7(23), 12856-12863, 2015.

2.        Qian Wang, Ru-Dong Li, Bin-Cheng Yin,* and Bang-Ce Ye*. Colorimetric Detection for Specific MicroRNA Based on Duplex-Specific Nuclease-Assisted Nanoparticle Amplification. Analyst 140, 6306-6312, 2015.

3.        Peng-Qiang Leng, Feng-Lan Zhao, Bin-Cheng Yin,* and Bang-Ce Ye*. A Novel, Colorimetric Method for Biogenic Amine Detection based on Arylalkylamine N-Acetyltransferase. Chemical Communication 51, 8712-8714, 2015.

4.        Bin-Cheng Yin, Shan Wu, Jin-Liang Ma, and Bang-Ce Ye*. A Novel Molecular Beacon-based Method for Isothermal Detection of Sequence-Specific DNA via T7 RNA Polymerase-Aided Target Regeneration. Biosensors & Bioelectronics 68, 365-370, 2015.

5.        Cui-Yuan Yu, Bin-Cheng Yin,* Shilong Wang, Zengguang Xu, and Bang-Ce Ye*. Improved Ligation-Mediated PCR Method Coupled with T7 RNA Polymerase for Sensitive DNA Detection. Analytical Chemistry 86, 7214-7218, 2014.

6.        Bin-Cheng Yin,* Jin-Liang Ma, Huynh-Nhu Le, Shilong Wang, Zengguang Xu, and Bang-Ce Ye*. A New Mode to Light Up Adjacent DNA-Scaffolded Silver Probe Pair and Its Application for Specific DNA Detection. Chemical Communication 50 (100), 15991-15994, 2014.

7.        Yuan-Chen He, Bin-Cheng Yin,* Lihua Jiang, and Bang-Ce Ye. Rapid Detection of MicroRNA based on p19-Enhanced Fluorescence Polarization. Chemical Communication 50 (47), 6236-6239, 2014.

8.        Xiaoyan Lin, Liang Cui, Yishun Huang, Ya Lin, Yi Xie, Zhi Zhu*, Bin-Cheng Yin, Xi Chen, and Chaoyong James Yang*. Carbon Nanoparticle-Protected Aptamers for Highly Sensitive and Selective Detection of Biomolecules based on Nuclease-Assisted Target Recycling Signal Amplification. Chemical Communication 50: 7646-7648, 2014.

9.        Jin Huang, Yong He, Xiaohai Yang, Kemin Wang, Le Ying, Ke Quan, Yanjing Yang, and Bin-Cheng Yin. I-Motif-Based Nano-Flares for Sensing pH Changes in Live Cells. Chemical Communication 50, 15768-15771, 2014.

10.    Bin-Cheng Yin, Yu-Qiang Liu, and Bang-Ce Ye*. Sensitive Detection of MicroRNA in Complex Biological Samples via Enzymatic Signal Amplification Using DNA Polymerase Coupled with Nicking Endonuclease. Analytical Chemistry 85 (23), 11487-11493, 2013.

11.    Cui-Yuan Yu, Bin-Cheng Yin,* and Bang-Ce Ye*. A Universal Real-Time PCR Assay for Rapid MicroRNA Quantification via the Enhancement of Base-Stacking Hybridization. Chemical Communication 49 (74), 8247-8249, 2013.

12.    Min Zhang, Yu-Qiang Liu, Cui-Yuan Yu, Bin-Cheng Yin,* and Bang-Ce Ye*. Multiplexed Detection of MicroRNAs by Tuning DNA-Scaffolded Silver Nanoclusters. Analyst 138 (17), 4812- 4817, 2013.

13.    Xin-Ping Wang, Bin-Cheng Yin,* and Bang-Ce Ye*. A Novel Fluorescence Probe of dsDNA-Templated Copper Nanoclusters for Quantitative Detection of MicroRNAs. RSC Advances 3, 8633-8636, 2013.

14.    Xin-Ping Wang, Bin-Cheng Yin,* Ping Wang, and Bang-Ce Ye*. Highly Sensitive Detection of MicroRNAs based on Isothermal Exponential Amplification-Assisted Generation of Catalytic G-Quadruplex DNAzyme. Biosensors & Bioelectronics 42, 131-135, 2013.

15.    Min Zhang, Huynh-Nhu Le, Xiao-Qin Jiang, Bin-Cheng Yin, and Bang-Ce Ye*.Time-Resolved Probes Based on Guanine/Thymine-Rich DNA-Sensitized Luminescence of Terbium (III). Analytical Chemistry 85 (23), 11665-11674, 2013.

16.    Bin-Cheng Yin, Yu-Qiang Liu, and Bang-Ce Ye*. One-step, Multiplexed Fluorescence Detection of microRNAs Based on Duplex-Specific Nuclease Signal Amplification. Journal of the American Chemical Society 134 (11), 5064-5067, 2012.

17.    Bin-Cheng Yin, Mingxu You, Weihong Tan, Bang-Ce Ye*. Mercury(II) Ions Detection via Pyrene-Mediated Photolysis of Disulfide Bonds. Chemistry - A European Journal 18(5), 1286-1289, 2012.

18.    Bin-Cheng Yin, Bang-Ce Ye*, Hui Wang, Zhi Zhu, Weihong Tan*. Colorimetric Logic Gates based on Aptamer-Crosslinked Hydrogel. Chemical Communication48, 1248-1250, 2012.

19.    Bin-Cheng Yin, Yi-Meng Guan, and Bang-Ce Ye*. An Ultrasensitive Electrochemical DNA Sensor based on the ssDNA-assisted Cascade of Hybridization Reaction. Chemical Communication 48 (35), 4208-4210, 2012.

20.    Yu-Qiang Liu, Min Zhang, Bin-Cheng Yin, and Bang-Ce Ye*. Attomolar Ultrasensitive MicroRNA Detection by DNA-Scaffolded Silver-Nanocluster Probe based on Isothermal Amplification. Analytical Chemistry 84(12), 5165-5169, 2012.

21.    Di Wu, Bin-Cheng Yin, (Co-author) and Bang-Ce Ye*. A Label-Free Electrochemical DNA Sensor based on Exonuclease III-Aided Target Recycling Strategy for Sequence-Specific Detection of Femtomolar DNA. Biosensors & Bioelectronics 28, 232-238, 2011.

22.    Mingxu You, Yan Chen, Lu Peng, Da Han, Bin-Cheng Yin, Bang-Ce Ye, and Weihong Tan*. Engineering DNA Aptamers for Novel Analytical and Biomedical Applications. Chemical Science 2, 1003-1010, 2011.

23.    Hui-Jie Tong, Na Jin, Bin-Cheng Yin, and Bang-Ce Ye*. A Novel Separation and Enrichment Method of 17β-Estradiol using Aptamer-Anchored Beads. Bioprocess and Biosystems Engineering 34, 189-195, 2011.

24.    Min Zhang, Bin-Cheng Yin, Weihong Tan, and Bang-Ce Ye*. A Versatile Graphene-Based Fluorescence “On/Off” Switch for Multiplex Detection of Various Targets. Biosensors & Bioelectronics 26, 3260-3265, 2011.

25.    Min Zhang, Bin-Cheng Yin, Xian-Fei Wang, and Bang-Ce Ye*. Interaction of Peptides with Graphene Oxide and Its Application for Real-Time Monitoring of Protease Activity. Chemical Communication 47, 2399-2401, 2011.

26.    Bin-Cheng Yin, Min Zhang, Weihong Tan*, and Bang-Ce Ye*. Peptide-Functionalized Spherical Polyelectrolyte Nanobrushes for Real-Time Sensing of Protease Activity. ChemBioChem 11, 494-497, 2010.

27.    Bin-Cheng Yin, Peng Zuo, Hao Huo, Xinhua Zhong, and Bang-Ce Ye*. DNAzyme-Self-Assembled Gold Nanoparticles for Determination of Metal Ions using Fluorescence Anisotropy Assay. Analytical Biochemistry 401, 47-52, 2010.

28.    Bin-Cheng Yin, Di Wu, and Bang-Ce Ye*. A Sensitive DNA-based Electrochemical Strategy for Trace Bleomycins Detection. Analytical Chemistry 82(19), 8272-8277, 2010.

29.    Bin-Cheng Yin, Bang-Ce Ye*, Hui Wang, Weihong Tan*, and Cong-Cong Xie. An Allosteric Dual-DNAzyme Unimolecular Probe for Colorimetric Detection of Copper(II). Journal of the American Chemical Society 131, 14624-14625, 2009.

30.    Bin-Cheng Yin, Xian-Fei Wang, and Ye Bang-Ce*. Multiplex Genotyping and Allele Frequency Estimation using Non-Gel Capillary Electrophoresis. Analytical Biochemistry 387, 221-229, 2009.

31.    Peng Zuo, Bin-Cheng Yin, (Co-author) Bang-Ce Ye*. DNAzyme-based Microarray for Highly Sensitive Detection of Metal Ions. Biosensors & Bioelectronics 25, 935-939, 2009.

32.    Sheng-Quan Jin, Bin-Cheng Yin, and Bang-Ce Ye*. A Multiplexed Bead-based Mesofluidic System for Foodborne Pathogen Bacteria Detection. Applied and Environmental Microbiology 75, 6647-6654, 2009.

33.    Bang-Ce Ye*, Yan Zhang, Hui Yu, Wen-Bang Yu, Bao-Hong Liu, Bin-Cheng Yin, Chun-Yun Yin, Yuan-Yuan Li, Ju Chu, Si-Liang Zhang. Time-resolved Transcriptome Analysis of Bacillus subtilis Responding to Valine, Glutamate, and Glutamine. PLoS ONE 4(9), e7073, 2009.

34.    Bang-Ce Ye*, Bincheng Yin. Highly Sensitive Detection of Mercury (II) ion by Fluorescence Polarization via Gold Nanoparticle Enhancement. Angewandte Chemie International Edition 47, 8386-8389, 2008.

35.    Bin-Cheng Yin, Honghua Li, and Bang-Ce Ye*. A Dual-Probe Hybridization Method for Reducing Variability in Single Nucleotide Polymorphism Analysis with Oligonucleotide Microarrays. Analytical Biochemistry 383, 270-278, 2008.

36.    Bin-Cheng Yin, Honghua Li, and Bang-Ce Ye*. Microarray-based Estimation of SNP Allele-frequency in Pooled DNA using the Langmuir Kinetic Model. BMC Genomics 9, 605, 2008.

37.    Bin-Cheng Yin, Fei Yue, and Bang-Ce Ye*. Construction of Microarrays for Genotyping of DQA using Unmodified 45-mer Oligonucleotide. Molecular Biotechnology 36, 142-150, 2007.

38.    Bang-Ce Ye, Min Zhang, Bin-Cheng Yin.《Nano-Bio Probe Design and Its Application for Biochemical Analysis》, Springer, 2012.