作者： 发布时间：2019-07-12 11:07:00
题目： The Quest for an Efficient Cell Factory for the Production of Recombinant Proteins: Pichia pastoris vs Yarrowia lipolytica
The non-conventional yeasts Y. lipolytica and P. pastoris are both well-established cell factories for the synthesis of recombinant proteins (rProt) in industry. Surprisingly, no direct comparison of their performances for rProt synthesis has been reported so far. Here, we report such a comparison using the CalB lipase from Candida antartica as a model protein. The corresponding codon optimized gene sequence was cloned under the control of strong inducible promoters, namely pEYKA3B and pAOX1, and expressed in recipient strains Y. lipolytica EYK1ko and P. pastoris MutS, respectively. With the resulting strains, cell growth, gene expression, carbon uptake rates and extracellular lipase activity were monitored over time during cultures in bioreactor in optimized conditions. Y. lipolytica performances were by far superior in terms of cell growth rate and extracellular lipase activity, although P. pastoris showed a significantly higher level of CalB gene expression. This lower protein productivity in P. pastoris was found related to the intracellular protein degradation by the proteasome complex. From this study, Y. lipolytica appears a more efficient cell factory that is in addition not related to the utilization of flammable methanol as inducer.
Prof Patrick Fickers
Gembloux Agro-Bio Tech, Microbial Processes and Interactions (MiPI)
Université de Liège, Belgium
Patrick Fickers has completed his PhD in Biochemistry from Universite de Liege, Belgium and from Institut National Agronomique, Paris, France. In 2004, he was at Polytech’ Lille, France as a Post doctorate. In 2005, he has joined the Centre of Protein Engineering, Liege, Belgium as FNRS fellow. He was an Associate Professor at Universite Libre de Bruxelles and the Head of the Biotechnology and Bioprocess Unit from 2009 to 2014. Since 2015, he is Professor at Gembloux Agro BioTech, University of Liege in the Microbial Processes and Interactions. He is also a Scientific Collaborator at University of Camaguey, Cuba and Adjunct Professor at TDT University (Ho Chi Minh City, Vietnam). He has published more than 40 research papers in peer-reviewed journals, 6 book chapters and several review articles. His researches focus on the development of yeast and bacterial strains by metabolic engineering. He is also interested in process development in bioreactor for the production of valuable compounds.
Main study areas
Development and optimization of production processes (biomass, metabolites, enzymes, pharmaceuticals) based on bacterial, fungal or animal cells culture in bioreactor
Industrial application fields
Micro-organisms production (bacteria, yeasts)