题目：New enzyme tools from uncharted natural product space
报 告人：苏黎世联邦理工学院 Jörn Piel 教授
Jörn Piel received a PhD in Chemistry at the University of Bonn, Germany, and
conducted postdoctoral work with Bradley Moore and Heinz Floss at the University
of Washington, Seattle. He then became Research Group Leader at the Max Planck
Institute of Chemical Ecology in Jena, Germany, and Associate Professor of Bioorganic
Chemistry at the University of Bonn. Since 2013 he is Full Professor of Microbiology
at ETH Zurich. Research of his lab focuses on metabolic functions of “microbial dark
matter”, the investigation and utilization of new biosynthetic enzymology, and ecologyand
genome-based methods of natural product discovery.
New enzyme tools from uncharted natural product space
Most areas of the bacterial tree of life are functionally uncharacterized. These regions include numerous deep-branching
taxa that lack cultivated representatives and live in diverse habitats. Our lab uses metagenomic and single-cell-based
mining strategies to investigate whether this massive taxonomic and ecological diversity is a resource of metabolic novelty.
We have previously reported uncultured symbionts of marine sponges as a rich source of bioactive and biosynthetically
unusual compounds.1,2 The talk will present recent insights into the metabolic repertoire of ‘Entotheonella’ sponge
symbionts, a “talented” producer taxon with a rich and diverse chemistry comparable to that of streptomycetes. While
most of the biosynthetic pathways had no counterparts in known cultured bacteria, functional studies also revealed
mechanistically surprising enzymes that expand the chemical space of ribosomal peptide biosynthesis and have
widespread homologs in culturable prokaryotes.3-5 Implications for synthetic biology applications will be discussed.
 M. Wilson et al., Nature 2014, 506, 58.
 J.B. Cahn et al., Proc. Natl. Acad. Sci. U. S. A. 2018, 115, 1718.
 M. F. Freeman et al., Science 2012, 338, 387.
 M. F. Freeman et al., Nat. Chem. 2017, 9, 387.
 B .I. Morinaka et al., Science 2018, 359, 779.