Jiang Pan

	Name: Jiang Pan
	Title: research assistant
	Phone: 021-64250840
	Fax: 021-64250840
	Email: panjiang@ecust.edu.cn

Education：

1996.09-2000.07: Bachelor of Biochemistry, East China University of Science and Technology

2000.09-2003.06: Master of Biochemical Engineering, East China University of Science and Technology

2007.09-       : Doctor of Fermentation Engineering, East China University of Science and Technology

Academic Positions: 2003-      ：School of Biotechnology, East China University of Science and Technology

Research：

1. Screening of microorganism and fermentation production of enzymes

2. Biocatalytic production of chiral compounds

3. Scale-up of biocatalytic process

Publication：

Journal papers：

1. Zhang ZJ, Pan J, Li CX, Yu HL, Zheng GW, Ju X, Xu JH*. Efficient production of (R)-(-)-mandelic acid using glutaraldehyde cross-linked Escherichia coli cells expressing Alcaligenes sp. nitrilase. Bioprocess & Biosystems Engineering, 2014. 37(7): 1241-1248

2. Zhang Y, Pan J, Luan ZJ, Xu GC, Park S, Xu JH*. Cloning and characterization of a novel esterase from Rhodococcus sp. for highly enantioselective synthesis of a chiral cilastatin precursor. Applied & Environmental Microbiology, 2014. 80(23): 7348-7355

3. Pan J, Zheng GW, Ye Q, Xu JH*. Optimization and scale-up of a bioreduction process for preparation of ethyl (S)-4-chloro-3-hydroxybutanoate. Organic Process Research & Development, 2014. 18(6): 739-743

4. Pan J, Dang ND, Zheng GW, Cheng B, Ye Q, Xu JH*. Efficient production of l-menthol in a two-phase system with SDS using an immobilized Bacillus subtilis esterase. Bioresources and Bioprocessing, 2014. 1: 12

5. Li J, Pan J, Zhang J, Xu JH*. Stereoselective synthesis of L-tert-leucine by a newly cloned leucine dehydrogenase from Exiguobacterium sibiricum. Journal of Molecular Catalysis B-Enzymatic, 2014. 105: 11-17

6. Hu K, Li CX, Pan J, Ni Y, Zhang XY, Xu JH*. Performance of a new thermostable mannanase in breaking guar-based fracturing fluids at high temperatures with little premature degradation. Applied Biochemistry & Biotechnology, 2014. 172(3): 1215-1226

7. Zhou DJ, Pan J, Yu HL, Zheng GW, Xu JH*. Target-oriented discovery of a new esterase-producing strain Enterobacter sp ECU1107 for whole cell-catalyzed production of (2S,3R)-3-phenylglycidate as a chiral synthon of Taxol. Applied Microbiology & Biotechnology, 2013. 97(14): 6293-6300

8. Zhang WX, Xu GC, Huang L, Pan J, Yu HL*, Xu JH*. Highly efficient synthesis of (R)-3-quinuclidinol in a space-time yield of 916 g l-1 d-1 using a new bacterial reductase ArQR. Organic Letters, 2013. 15(19): 4917-4919

9. Ngo-Thi MT, Yin JG, Pan J, Zheng GW*, Xu JH*. Enantioselective hydrolysis of dl-menthyl benzoate by cell-free extract of newly isolated Acinetobacter sp. ECU2040. Applied Biochemistry & Biotechnology, 2013. 170(8): 1974-1981

10. Ma BD, Yu HL*, Pan J, Liu JY, Ju X, Xu JH*. A thermostable and organic-solvent tolerant esterase from Pseudomonas putida ECU1011: Catalytic properties and performance in kinetic resolution of α-hydroxy acids. Bioresource Technology, 2013. 133: 354-360

11. Liu JY, Zheng GW*, Li CX, Yu HL, Pan J, Xu JH*. Multi-substrate fingerprinting of esterolytic enzymes with a group of acetylated alcohols and statistic analysis of substrate spectrum. Journal of Molecular Catalysis B-Enzymatic, 2013. 89: 41-47

12. Liu CH, Pan J, Ye Q, Xu JH*. Enzymatic production of Cilastatin intermediate via highly enantioselective hydrolysis of methyl (±)-2,2-dimethylcyclopropane carboxylate using newly isolated Rhodococcus sp. ECU1013. Applied Microbiology & Biotechnology, 2013. 97(17): 7659-7667

13. Ju X, Yu HL, Pan J*, Xu JH*. Improved production of Pseudomonas sp. ECU1011 acetyl esterase by medium design and fed-batch fermentation. Bioprocess & Biosystems Engineering, 2012. 35(3): 323-331

14. Zheng GW, Yu HL, Li CX, Pan J, Xu JH*. Immobilization of Bacillus subtilis esterase by simple cross-linking for enzymatic resolution of dl-menthyl acetate. Journal of Molecular Catalysis B-Enzymatic, 2011. 70(3-4): 138-143

15. Zhao J, Chu YY, Li AT, Ju X, Kong XD, Pan J, Tang Y, Xu JH*. An unusual (R)-selective epoxide hydrolase with high activity for facile preparation of enantiopure glycidyl ethers. Advanced Synthesis & Catalysis, 2011. 353(9): 1510-1518

16. Zhang ZJ, Pan J, Liu JF, Xu JH*, He YC, Liu YY. Significant enhancement of (R)-mandelic acid production by relieving substrate inhibition of recombinant nitrilase in toluene-water biphasic system. Journal of Biotechnology, 2011. 152(1-2): 24-29

17. Pan J, Kong XD, Li CX, Ye Q, Xu JH*, Imanaka T. Crosslinking of enzyme coaggregate with polyethyleneimine: A simple and promising method for preparing stable biocatalyst of Serratia marcescens lipase. Journal of Molecular Catalysis B-Enzymatic, 2011. 68(3-4): 256-261

18. Pan J, Yu HL, Xu JH, Lin GQ. Advances in biocatalysis: Enzymatic reactions and their applications. Asymmetric catalysis from a chinese perspective, 2011. 36: 67-103

19. Liu JF, Zhang ZJ, Li AT, Pan J, Xu JH*. Significantly enhanced production of recombinant nitrilase by optimization of culture conditions and glycerol feeding. Applied Microbiology & Biotechnology, 2011. 89(3): 665-672

20. Li AT, Zhang JD, Yu HL, Pan J, Xu JH*. Significantly improved asymmetric oxidation of sulfide with resting cells of Rhodococcus sp. in a biphasic system. Process Biochemistry, 2011. 46(3): 689-694

21. Li AT, Yu HL, Pan J, Zhang JD, Xu JH*, Lin GQ. Resolution of racemic sulfoxides with high productivity and enantioselectivity by a Rhodococcus sp. strain as an alternative to biooxidation of prochiral sulfides for efficient production of enantiopure sulfoxides. Bioresource Technology, 2011. 102(2): 1537-1542

22. Zheng GW, Pan J, Yu HL, Ngo-Thi MT, Li CX, Xu JH*. An efficient bioprocess for enzymatic production of l-menthol with high ratio of substrate to catalyst using whole cells of recombinant E. coli. Journal of Biotechnology, 2010. 150(1): 108-114

23. Zhao LL, Pan J, Xu JH*. Efficient production of diltiazem chiral intermediate using immobilized lipase from Serratia marcescens. Biotechnology and Bioprocess Engineering, 2010. 15(2): 199-207

24. Zhang X, Xu JH*, Liu DH, Pan J, Chen B. Construction and operation of a fibrous bed reactor with immobilized lactonase for efficient production of (R)-α-hydroxy-γ-butyrolactone. Biochemical Engineering Journal, 2010. 50(1-2): 47-53

25. Liu X, Xu JH*, Pan J, Zhao J. Efficient production of (S)-naproxen with (R)-substrate recycling using an overexpressed carboxylesterase BsE-NP01. Applied Biochemistry & Biotechnology, 2010. 162(6): 1574-1584

26. Ju X, Yu HL, Pan J, Wei DZ, Xu JH*. Bioproduction of chiral mandelate by enantioselective deacylation of α-acetoxyphenylacetic acid using whole cells of newly isolated Pseudomonas sp. ECU1011. Applied Microbiology & Biotechnology, 2010. 86(1): 83-91

27. Chang L, Ouyang LM, Xu Y, Pan J, Xu JH*. Highly enantioselective hydrolysis of phenyl-1,2-ethanediol cyclic carbonates by newly isolated Bacillus sp. ECU0015. Journal of Molecular Catalysis B-Enzymatic, 2010. 66(1-2): 95-100

28. Zhu H, Pan J, Hu B, Yu HL, Xu JH*. Immobilization of glycolate oxidase from Medicago falcata on magnetic nanoparticles for application in biosynthesis of glyoxylic acid. Journal of Molecular Catalysis B-Enzymatic, 2009. 61(3-4): 174-179

29. Hu B, Pan J, Yu HL, Liu JW, Xu JH*. Immobilization of Serratia marcescens lipase onto amino-functionalized magnetic nanoparticles for repeated use in enzymatic synthesis of Diltiazem intermediate. Process Biochemistry, 2009. 44(9): 1019-1024

30. Zhao LL, Xu JH*, Zhao J, Pan J, Wang ZL. Biochemical properties and potential applications of an organic solvent-tolerant lipase isolated from Serratia marcescens ECU1010. Process Biochemistry, 2008. 43(6): 626-633

31. Yang W, Xu JH*, Pan J, Xu Y, Wang ZL. Efficient reduction of aromatic ketones with NADPH regeneration by using crude enzyme from Rhodotorula cells and mannitol as cosubstrate. Biochemical Engineering Journal, 2008. 42(1): 1-5

32. Ou L, Xu Y, Ludwig D, Pan J, Xu JH*. Chemoenzymatic deracemization of chiral secondary alcohols: Process optimization for production of (R)-1-indanol and (R)-1-phenylethanol. Organic Process Research & Development, 2008. 12(2): 192-195

33. He YC, Xu JH*, Pan J, Ouyang LM, Xu Y. Preparation of (R)-(-)-mandelic acid and its derivatives from racemates by enantioselective degradation with a newly isolated bacterial strain Alcaligenes sp. ECU0401. Bioprocess & Biosystems Engineering, 2008. 31(5): 445-451

34. Zhang X, Xu JH*, Xu Y, Pan J. Isolation and properties of a levo-lactonase from Fusarium proliferatum ECU2002: a robust biocatalyst for production of chiral lactones. Applied Microbiology & Biotechnology, 2007. 75(5): 1087-1094

35. Long ZD, Xu JH*, Zhao LL, Pan J, Yang S, Hua L. Overexpression of Serratia marcescens lipase in Escherichia coli for efficient bioresolution of racemic ketoprofen. Journal of Molecular Catalysis B-Enzymatic, 2007. 47(3-4): 105-110

36. Long ZD, Xu JH*, Pan J. Immobilization of Serratia marcescens lipase and catalytic resolution of trans-3-(4'-methoxyphenyl)glycidic acid methyl ester. Chinese Journal of Catalysis, 2007. 28(2): 175-179

37. Long ZD, Xu JH*, Pan J. Significant improvement of Serratia marcescens lipase fermentation by optimizing medium, induction and oxygen supply. Applied Biochemistry & Biotechnology, 2007. 142(2): 148-157

38. He YC, Xu JH*, Xu Y, Ouyang LM, Pan J. Biocatalytic synthesis of (R)-(-)-mandelic acid from racemic mandelonitrile by a newly isolated nitrilase-producer Alcaligenes sp. ECU0401. Chinese Chemical Letters, 2007. 18(6): 677-680

39. Xu W, Xu JH*, Pan J, Gu Q, Wu XY. Enantioconvergent hydrolysis of styrene epoxides by newly discovered epoxide hydrolases in mung bean. Organic Letters, 2006, 8(8): 1737-1740

40. Huang HR, Xu JH*, Xu Y, Pan J, Liu X. Preparation of (S)-mandelic acids by enantioselective degradation of racemates with a new isolate Pseudomonas putida ECU1009. Tetrahedron-Asymmetry, 2005, 16(12): 2113-2117

41. Xu Y, Xu JH*, Pan J, Zhao L, Zhang SL. Biocatalytic resolution of nitro-substituted phenoxypropylene oxides with Trichosporon loubierii epoxide hydrolase and prediction of their enantiopurity variation with reaction time. Journal of Molecular Catalysis B-Enzymatic, 2004, 27(4-6): 155-159

42. Xu Y, Xu JH*, Pan J, Tang YF. Biocatalytic resolution of glycidyl aryl ethers by Trichosporon loubierii: cell/substrate ratio influences the optical purity of (R)-epoxides. Biotechnology Letters, 2004, 26(15): 1217-1221

43. Chen Y, Xu JH*, Pan J, Xu Y, Shi JB. Catalytic resolution of (RS)-HMPC acetate by immobilized cells of Acinetobacter sp. CGMCC 0789 in a medium with organic cosolvent. Journal of Molecular Catalysis B-Enzymatic, 2004, 30(5-6): 203-208

44. Pan J, Xu JH*. Marked enhancement of epoxide hydrolase production from Trichosporon loubierii ECU1040 by substrate induction and fed-batch fermentation. Enzyme and Microbial Technology, 2003, 33(4): 527-533

45. Yang S, Zhou LP, Tang HX, Pan J, Wu XJ, Huang H, Yuan ZY*. Rational design of a more stable penicillin G acylase against organic cosolvent. Journal of Molecular Catalysis B-Enzymatic, 2002, 18: 285-290

Patent：

1. Yu HL, Zhang WX, Pan J, Xu JH. Sequence of Agrobacterium radiobacter quininone reductase and its use in asymmetric synthesis of (R)-3-quinuclidinol. ZL201310422722.1, Peop. Rep. China. p. 19pp.

2. Xu JH, Hu K, Li CX, Pan J. Sequence and expression of mannanase from Dictyoglomus thermophilum and its use biological gel breaker of oil well water-based fracturing fluid. ZL 201310283115.1, Peop. Rep. China . p. 26pp.

3. Xu JH, Ma BD, Yu HL, Pan J. Cloning of gene rPPE01 encoding acetylated hydroxy acid hydrolase from Pseudomonas and its application. ZL201310011788.1, Peop. Rep. China. p. 24pp

4. Xu JH, Zhang CS, Pan J, Li CX, Zhang ZJ. DNA and protein sequences of Labrenzia aggregata nitrilase and its use for production of (R)-o-chloromandelic acid. ZL201210044367.4, Peop. Rep. China. p. 26pp.

5. Kong XD, Xu JH, Zhou JH, Pan J. Mutants of epoxide hydrolase BmEH of Bacillus megaterium and their use in chiral separation of racemic epoxides. ZL201310011777.3, Peop. Rep. China. p. 31pp.

6.Xu JH, Zhou DJ, Pan J, Li CX, Yu HL, Zheng GW. An esterase-producing Enterobacter strain, the esterase, its gene and their application in preparing chiral precursor of taxol. ZL201210339852.4, Peop. Rep. China. p. 22pp.

7.Xu JH, Liu CH, Pan J, Yu HL. Application of Rhodococcus sp. ECU 1013 for preparing (S)-(+)-2,2-dimethyl cyclopropanecarboxylic acid. ZL201210276185.X, Peop. Rep. China. p. 20pp.

8. Ma HM, Li CX, Pan J, Zheng GW, Yu HL, Xu JH. Cloning of gene for carbonyl reductase of Candida glabrata, its mutants and use for in asymmetric reduction of carbonyl compounds. ZL201210136823.8, Peop. Rep. China. p. 23pp.

9.Xu JH, Ni Y, Li CX, Zhang J, Pan J, Wang LJ. Cloning of reductase gene from Bacillus, 2011, East China University of Science and Technology. ZL201210050842.9, Peop. Rep. China. p. 30pp.

10. Xu JH, Liu X, Pan J, Zhao J. Method for manufacturing (S)-naproxen by enzymatic resolution of racemic naproxen ester. ZL201010114462.8, Peop. Rep. China. p. 9pp.

11. Xu JH, Wang LJ, Pan J, Ni Y, Shen ND, Qiu YJ, Qiu LH. Application of oxidoreductase of Streptomyces coelicolor in asymmetric reduction of prochiral carbonyl compounds. ZL201010599376.0, Peop. Rep. China. p. 18pp.

12. Xu JH, Shen ND, Qian XL, Zhang J, Zheng GW, Li CX, Pan J. Sequence of Candida glabrata carbonyl reductase and its use in asymmetric reduction of carbonyl compound. ZL201110387573.0, Peop. Rep. China. p. 22pp.

13.Xu JH, Ni Y, Pan J, Li CX, Ma HM. Method for manufacturing methyl (R)-o-chloromandelate by asymmetric reduction with biocatalyst of Escherichia coli transformed with aldo-keto reductase and glucose dehydrogenase genes. ZL201110098532.X, Peop. Rep. China. p. 18pp.

14. Xu JH, Zhao J, Li AT, Pan J. Cloning and application of epoxide hydrolase gene from Bacillus megaterium. ZL201110091546.9, Peop. Rep. China. p. 22pp.

15. Xu JH, Zhang X, Pan J, Xu Y, Method for manufacturing chiral hydroxyacid with bacterial strain capable of producing levorotatory lactone hydrolase. ZL200780038340.1, Peop. Rep. China. p. 18 pp.

16.Xu JH, Zhang ZJ, Liu JF, Pan J. DNA and protein sequences of Alcaligenes nitrilase and its use for preparation of R-(-)-mandelic acid. ZL200910049506.0, Peop. Rep. China. p. 11pp.

17.Xu JH, Chang L, Pan J, Xu Y. Bacillus and its application as biocatalyst for hydrolysis. ZL200910054358.1, Peop. Rep. China. p. 9pp.

18. Xu JH, Ju X, Pan J. Esterase of Pseudomonas ECU1011, and its application in preparing mandelic acid and its derivatives with optical purity. ZL200910049768.7, Peop. Rep. China. p. 11pp.

19.Xu JH, Zheng GW, Pan J, Yu HL. Bacillus subtilis esterase and application of said esterase in producing L-menthol. ZL200810040988.9, Peop. Rep. China. p. 11 pp.

20. Xu JH, Pan J, Yang W, Lu BR, Zhu YZ. Method for preparing optically pure chiral sec-alcohol with Rhodotorula cells as catalyst. ZL200610028357.6, Peop. Rep. China. p. 10 pp.

21. Xu J, He Y, Pan J, Xu Y, Alcaligenes and method for manufacturing optically active, 2008, East China University of Science and Technology, Peop. Rep. China. p. 10 pp.

22. Xu JH, Huang HR, Xu Y, Pan J. Application of Bacillus and Pseudomonas putida in degrading racemic mandelic acid. ZL200610082377.1, Peop. Rep. China. p. 7 pp.

23. Xu JH, Gao L, Pan J, Long ZD, Serratia sp. ECU1010 and its application in preparing chiral precursor of diltiazem. ZL200410067046.1, Peop. Rep. China. p. 10 pp.