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Faculty

Chunxiu Li

Name: Chunxiu Li
Title: Associate Professor
Phone:  021-64250840
Fax:  021-64250840
Email:  chunxiuli@ecust.edu.cn
Research:
Screening of novel enzymes;
Researches on enzyme library construction.
Publication:
Journal papers:
1. Zheng MM, Wang RF, Li CX, Xu JH* (2015). Two-step enzymatic synthesis of ursodeoxycholic acid with a new 7β-hydroxysteroid dehydrogenase from Ruminococcus torques. Process Biochemistry, 50: 598-604.
2. Wang RF, Zheng MM, Cao YD, Li H, Li CX, Xu JH*, Wang ZT* (2015). Biotransformation of vina-ginsenoside R7 to rare notoginsenoside ST-4 using a new recombinant glycoside hydrolase from Herpetosiphon aurantiacus. Appl. Microbiol. Biotechnol., 99: 3433-3442.
3. Gong Y, Xu GC, Li CX*, Zheng GW, Xu JH* (2014). A thermostable variant of Bacillus subtilis esterase: characterization and application for resolving dl-menthyl acetate. J Mol Catal B: Enzym. 109: 1-8.
4. Liu XY, Li CX, Luo XJ, Lai QL, Xu JH* (2014). Burkholderia jiangsuensis sp. nov., a methyl parathion (MP)-degrading bacterium, isolated from MP-contaminated soil. Int J Syst Evol Microbiol, 64: 3247-3253.
5. Liu XY, Luo XJ, Li CX, Lai QL, Xu JH* (2014). Draft genome sequence of Burkholderia sp. strain MP-1, a methyl parathion (MP)-degrading bacterium from MP-contaminated soil. Genome Announcements, 2(3): e00344-14
6. Du FL, Yu HL, Xu JH, Li CX* (2014). Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E. coli Bioresources and Bioprocessing, 2014, 1: 10.
7. Zhang ZJ, Pan J, Li CX, Yu HL, Ju X, Xu JH* (2014). Efficient production of (R)-(-)-mandelic acid using glutaraldehyde cross-linked Escherichia coli cells expressing Alcaligenes sp. nitrilase. Bioprocess and Biosystems Engineering. 37: 1241–1248.
8. Hu K, Li CX*, Pan J, Ni Y, Zhang XY, Xu JH* (2014). Performance of a new thermostable mannanase in breaking guar-based fracturing fluids at high temperatures with little premature degradation. Applied Biochemistry and Biotechnology, 172(3): 1215-1226.
9. Liu JY, Zheng GW*, Li CX, Yu HL, Pan J, Xu JH* (2013). Multi-substrate fingerprinting of esterolytic enzymes with a group of acetylated alcohols and statistic analysis of substrate spectrum. Journal of Molecular Catalysis B: Enzymatic 89: 41–47.
10. Ma HM, Yang LL, Ni Y, Zhang J, Li CX, Zheng GW, Yang HY*, Xu JH* (2012). Stereospecific reduction of methyl o-chlorobenzoylformate at 300 g/L without additional cofactor using a carbonyl reductase mined from Candida glabrata. Advanced Synthesis & Catalysis, 354, 17651772.
11. Shen ND, Ni Y, Ma HM, Wang LJ, Li CX, Zheng GW, Zhang J, Xu JH* (2012). Efficient synthesis of a chiral precursor for ACE inhibitors in high space-time yield by a new reductase without external cofactors. Organic Letters, 14 (8): 19821985.
12. Zou ZZ, Yu HL*, Li CX, Zhou XW, Hayashi C, Sun J, Liu BH, Imanaka T, Xu JH*. (2012). A new thermostable β-glucosidase mined from Dictyoglomus thermophilum: Properties and performance in octyl glucoside synthesis at high temperatures. Bioresource Technology, 118: 425430.
13. Ni Y, Pan J, Ma HM, Li CX, Zhang J, Zheng GW, Xu JH* (2012). Bioreduction of methyl o-chlorobenzoylformate at 500 g L1 without external cofactors for efficient production of enantiopure clopidogrel intermediate. Tetrahedron Letters, 53: 4715–4717.
14. Zhang CS, Zhang ZJ, Li CX, Yu HL, Zheng GW*, Xu JH* (2012). Efficient production of (R)-o-chloromandelic acid by deracemization of o-chloromandelonitrile with a new nitrilase mined from Labrenzia aggregate. Appl Microbiol Biotechnol, 95: 91–99.
15. Wang D, Sun J, Yu, HL, Li CX, Bao J, Xu JH* (2012). Maximum saccharification of cellulose complex by an enzyme cocktail supplemented with cellulase from newly isolated Aspergillus fumigatus ECU0811. Applied Biochemistry and Biotechnology, 166 (1): 176186.
16. Liu JM, Xin XJ, Li CX, Xu JH, Bao J * (2012). Cloning of thermostable cellulase genes of Clostridium thermocellum and their secretive expression in Bacillus subtilis. Applied Biochemistry and Biotechnology, 166 (3): 652662.
17. Wang QQ, Yu HL*, Zhao N, Li CX, Shang YZ, Liu HL, Xu JH* (2012). Significantly improved equilibrium yield of long-chain alkyl glucosides via reverse hydrolysis in a water-poor system using cross-linked almond meal as a cheap and robust biocatalyst. Chinese Journal of Catalysis, 33 (2): 275280.
18. Li AT, Zheng GW, Yu HL, Pan J, Li CX, Xu JH (2012). Preparation of enantiopure sulfoxides by enantioselelctive oxidation with whole-cells of Rhodococcus sp. ECU0066. In: Practical Methods for Biocatalysis and Biotransformations 2, Eds. J. Whittall and P.W. Sutton, Wiley 2012, UK. Chapter 11, Section 11.3, pp. 307310.
19. Ni Y, Li CX*, Zhang J, Shen ND, Bornscheuer U, Xu JH* (2011). Efficient reduction of ethyl 2-oxo-4-phenylbutyrate at 620 g/L by a bacterial reductase with broad substrate spectrum. Advanced Synthesis and Catalysis, 353: 12131217.
20. Wang LJ, Li CX*, Ni Y, Zhang J, Liu X, Xu JH* (2011). Highly efficient synthesis of chiral alcohols with a novel NADH-dependent reductase from Streptomyces coelicolor. Bioresource Technology, 102 (14): 70237028.
21. Ni Y, Li CX*, Ma HM, Zhang J, Xu JH* (2011). Biocatalytic properties of a recombinant aldo-keto reductase with broad substrate spectrum and excellent stereoselectivity. Applied Microbiology and Biotechnology, 89: 1111-1118.
22. Ni Y, Li CX,* Wang LJ, Zhang J, Xu JH* (2011). Highly stereoselective reduction of prochiral ketones by a bacterial reductase coupled with cofactor regeneration. Organic and Biomolecular Chemistry, 9 (15): 5463–5468.
23. Qian L, Liu JY, Liu JY, Yu HL, Li CX, JH Xu* (2011). Fingerprint lipolytic enzymes with chromogenic p-nitrophenyl esters of structurally diverse carboxylic acids. Journal of Molecular Catalysis B Enzymatic, 73: 22–26.
24. Zheng GW, Yu HL, Li CX, Pan J, Xu JH* (2011). Immobilization of Bacillus subtilis esterase by simple cross-linking for enzymatic resolution of dl-menthyl acetate. Journal of Molecular Catalysis B: Enzymatic, 70: 138143.
25. Pan J, Kong XD, Li CX, Ye Q, Xu JH*, Imanaka T (2011). Crosslinking of enzyme coaggregate with polyethyleneimine: A simple and promising method for preparing stable biocatalyst of Serratia marcescens lipase. Journal of Molecular Catalysis B: Enzymatic, 68 (3-4): 256261.
26. Tian X, Zheng GW*, Li CX, Wang ZL, Xu JH* (2011). Enantioselective production of (S)-1-phenyl-1,2-ethanediol from dicarboxyesters by recombinant Bacillus subtilis esterase. Journal of Molecular Catalysis B Enzymatic, 73 (1-4): 80–84.
27. Ju X, Pan J, Yu HL, Li CX, Xu JH* (2011). Improving Pseudomonas sp. esterase performance by engineering approaches for kinetic resolution of 2-acetoxyphenylacetic acids. Biochemical Engineering Journal, 57: 63–68.
28. Shi QQ, Sun J, Yu HL, Li CX, Bao J, Xu JH* (2011). Catalytic performance of corn stover hydrolysis by a new isolate Penicillium sp. ECU0913 producing both cellulase and xylanase. Applied Biochemistry and Biotechnology, 164 (6): 819830.
29. Qiu LH, Li CX*, Sun J, Wang ZL, Ye Q, Xu JH* (2011). Thermostable bacterial endoglucanases mined from Swiss-Prot database. Applied Biochemistry and Biotechnology, 165 (7-8): 14731484.
30. Zheng GW, Pan J, Yu HL, Ngo-Thi MT, Li CX, Xu JH* (2010). An efficient bioprocess for enzymatic production of l-menthol with high ratio of substrate to catalyst using whole cells of recombinant E. coli. Journal of Biotechnology, 150: 108–114.
Achievement:
Patent::
1. 许建和、胡可、李春秀、潘江。甘露聚糖酶及其基因和应用。专利号:ZL 201310283115.1,授权日:2015.02.25
2. 许建和、张陈胜、潘江、李春秀、张志钧。一种腈水解酶及其基因和应用。专利号:ZL201210044367.4,授权日:2014.10.01
3. 马宏敏、李春秀、潘江、郑高伟、郁惠蕾、许建和。一种羰基还原酶、基因和突变体及在不对称还原羰基化合物中的应用。专利号:ZL201210136823.8,授权日:2014.06.11
4. 许建和、倪燕、李春秀、张杰、潘江、王丽娟。一种还原酶及其基因、重组酶及制备方法和应用。专利号:ZL201210050842.9,授权日:2014.02.12
5. 许建和、沈乃东、钱小龙、张杰、郑高伟、李春秀、潘江。一种羰基还原酶及其基因和在不对称还原羰基化合物中的应用。专利号:ZL201110387573.0,授权日:2013.06.26
6. 许建和、倪燕、潘江、李春秀、马宏敏。生物催化不对称还原制备(R)-邻氯扁桃酸甲酯的方法。专利号:ZL201110098532.X,授权日:2013.06.26
7. 许建和、倪燕、李春秀、张杰、潘江、王丽娟。一种还原酶及基因、重组酶及制备方法和应用。专利号:ZL201010527409.0,授权日:2013.04.24
Copyright: 2015 Biocatalysis and Biotechnology Laboratory synthesis Address: Shanghai Mei Long Road 130, 18th Floor, East China University of experiment, 9th Floor, East Email: jianhexu @ ecust. edu.cn      
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