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教师

张志钧


姓名:张志钧
职称:讲师
电话:021-64250840
传真:021-64250840
邮箱:zjzhang@ecust.edu.cn
学习经历:
2002/9-2006/7    内蒙古科技大学   生物工程             工学学士
2006/9-2013/3    华东理工大学     生物化工             工学博士
2010/9-2012/9    明尼苏达大学     生物化学与生物技术   联合培养博士
工作经历:
2013/4-2015/4    华东理工大学     师资博士后
2015/4-          华东理工大学     讲师

研究方向:
新型生物催化剂的发现、设计、分子改造及应用研究
发表论文:
期刊论文:
1. Zhi-Jun Zhang, Hui-Lei Yu, Tadayuki Imanaka, Jian-He Xu*. Efficient production of (R)-(-)-mandelic acid by isopropanol-permeabilized recombinant E. coli cells expressing Alcaligenes sp. nitrilase. Biochem. Eng. J. 2015, 95: 71-77.
2. Zhi-Jun Zhang, Jiang Pan, Bao-Di Ma, Jian-He Xu*. Efficient biocatalytic synthesis of chiral chemicals. Adv. Biochem. Eng./Biotechnol. In press
3. Zhi-Jun Zhang, Jiang Pan, Chun-Xiu Li, Hui-Lei Yu, Gao-Wei Zheng, Xin Ju, Jian-He Xu*. Efficient production of (R)-(-)-mandelic acid using glutaraldehyde cross-linked Escherichia coli cells expressing Alcaligenes sp. nitrilase. Bioprocess Biosyst. Eng. 2014, 37: 1241-1248.
4. 张志钧, 许国超, 许建和. 精细化学品绿色合成用工具酶的定向开发. 生物产业技术. 2013, 6: 12-19.
5. Zhi-Jun Zhang, Jiang Pan, Jun-Feng Liu, Jian-He Xu*, Yu-Cai He, You-Yan Liu. Significant enhancement of (R)-mandelic acid production by relieving substrate inhibition of recombinant nitrilase in toluene-water biphasic system. J. Biotechnol. 2011, 152: 24-29.
6. Zhi-Jun Zhang, Jian-He Xu*, Yu-Cai He, Li-Ming Ouyang, You-Yan Liu. Cloning and biochemical properties of a highly thermostable and enantioselective nitrilase from Alcaligenes sp. and its potential for (R)-(-)-mandelic acid production. Bioprocess Biosyst. Eng. 2011, 34: 315-322.
7. Zhi-Jun Zhang, Jian-He Xu*, Yu-Cai He, Li-Ming Ouyang, You-Yan Liu, Tadayuki Imanaka. Efficient production of (R)-(-)-mandelic acid with highly substrate/product tolerant and enantioselective recombinant Alcaligenes sp. nitrilase. Process Biochem. 2010, 45: 887-891.
8. Bao-Di Ma, Xu-Dong Kong, Hui-Lei Yu*, Zhi-Jun Zhang, Shuai Dou, Yan-Peng Xu, Yan Ni, Jian-He Xu*. Increased catalyst productivity in α-hydroxy acids resolution by esterase mutation and substrate modification. ACS Catal. 2014, 4: 1026-1031.
9. Guo-Chao Xu, Hui-Lei Yu*, Zhi-Jun Zhang, Jian-He Xu*. Stereocomplementary bioreduction of β-ketonitrile without ethylated byproduct. Org. Lett. 2013, 15(21): 5408-5411.
10. Gao-Wei Zheng, Xu-Yun Liu, Zhi-Jun Zhang, Ping Tian, Guo-Qiang Lin, Jian-He Xu*. Separation of enantiopure m-substituted 1-phenylethanols in high space-time yield using Bacillus subtilis esterase. RSC Adv. 2013, 3: 20446-20449.
11. Chen-Sheng Zhang, Zhi-Jun Zhang, Chun-Xiu Li, Hui-Lei Yu, Gao-Wei Zheng, Jian-He Xu*. Efficient production of (R)-o-chloromandelic acid by deracemization of o-chloromandelonitrile with a new nitrilase mined from Labrenzia aggregata. Appl. Microbiol. Biotechnol. 2012, 95: 91-99.
12. Jun-Feng Liu, Zhi-Jun Zhang, Ai-Tao Li, Jiang Pan, Jian-He Xu*. Significantly enhanced production of recombinant nitrilase by optimization of culture conditions and glycerol feeding. Appl. Microbiol. Biotechnol. 2011, 89: 665-672.
13. Yu-Cai He, Zhi-Jun Zhang, Jian-He Xu*, You-Yan Liu. Biocatalytic synthesis of (R)-(-)-mandelic acid from racemic mandelonitrile by cetyl-trimethylammoniumbromide-permeabilized cells of Alcaligenes faecalis ECU0401. J. Ind. Microbiol. Biotechnol. 2010, 37: 741-750.
会议论文:
1. 张志钧,许建和。重组大肠杆菌的通透、交联固定化及在合成手性扁桃酸的应用。第六届全国发酵工程学术研讨会,2014年6月18日-20日,无锡.
2. Zhi-Jun Zhang, Jian-He Xu*, Romas J. Kazlauskas*. Construction of ancestral enzymes for unnatural reactions. Enzyme Engineering XXII, September 22-26, 2013, Toyama, Japan.
3. Zhi-Jun Zhang, Bryan Jones, Antony Dean, Romas J. Kazlauskas*. Sequence-based reconstruction of ancestral enzymes to find catalytically promiscuous enzyme. 245th National Meeting of the American-Chemical-Society, April 7-11, 2013, New Orleans, USA.
4. Romas J. Kazlauskas*, Zhi-Jun Zhang, Bryan Jones, Jan von Langermann. Reconstruction of catalytically promiscuous ancestral enzymes as catalysts for unnatural reactions. 245th National Meeting of the American-Chemical-Society, April 7-11, 2013, New Orleans, USA.
5. 张志钧、Romas J. Kazlauskas*、许建和*. 羟腈裂解酶的理性改造研究。长三角“先进生物制造-基础与应用”博士生论坛,2012,11月8日-10日,南京.
6. Zhi-Jun Zhang, Jian-He Xu, Romas J. Kazlauskas. Engineering an esterase into a nitroaldolase. Gordon Research Conference: Biocatalysis, July 8-13, 2012, Smithfield, USA.
7. 张志钧,许建和。利用重组腈水解酶高效制备光学纯(R)-(-)-扁桃酸。第三届长三角研究生生物技术论坛。上海,2010年7月24-25日.
8. Zhi-Jun Zhang, Jian-He Xu. Markedly enhanced production of (R)-(-)-mandelic acid using recombinant nitrilase in a biphasic system. The 15th Symposium of Young Asian Biochemical Engineers' Community, Xiamen, Dec 18-20, 2009.
9. Zhi-Jun Zhang, Jian-He Xu, Yu-Cai He. Cloning and expression of a nitrilase gene from Alcaligenes sp. strain ECU0401 for production of (R)-(-)-mandelic acid. 12th, APCChE Congress, Dalian, August 4-5, 2008.
专著
研究成果:
近年主持的科研项目:
1. 国家自然科学基金青年基金 (21406067)  新型腈还原酶的资源挖掘及构效关系研究  2015-2017  25万
2. 中国博士后科学基金 (2014M560309) 腈还原酶的基因挖掘及结构-功能关系研究 2014-2015  8万
3. 中央高校基本科研业务费 (222201314016) 卤醇脱卤酶祖先酶的设计和改造及其在大分子洛尔类药物前体合成中的应用研究  2013-2015  12万
4. 横向项目  口含烟原料发酵菌群分离培养及应用  2014-2015  45万
申请专利:
1. 许建和,张志钧,刘俊峰,潘江。一种编码产碱杆菌腈水解酶的基因及其用于制备扁桃酸单一对映体的方法。中国专利,专利号:ZL 200910049506.0
2. 许建和,何玉财,张志钧。一种产碱杆菌的培养及用于腈水解制备羟基乙酸的方法。中国专利,专利号:ZL 200710048010.2
3. 许建和、张陈胜、潘江、李春秀、张志钧. 一种腈水解酶及其基因和应用。中国专利,专利号:ZL 2012100443674
4. 许建和、罗晓晶、潘江、张志钧. 一种有机磷酯水解酶、基因、重组表达转化体及其应用. 中国专利,申请号:201410167955.6
5. 许建和、张龑、栾政娇、潘江、郁惠蕾、张志钧. 一种环丙烷甲酸酯水解酶、基因、突变体及其应用. 中国专利,申请号:201410363834.9
人才奖项:
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