郑高伟

	姓名：郑高伟
	职称：教授
	电话：021-64250840
	传真：021-64250840
	邮箱：gaoweizheng@ecust.edu.cn

学习经历：

2005.09-2010.08   华东理工大学   生物化工硕博连读               博士

2001.09-2005.07   四川大学       生物工程专业                   学士

工作经历：

2013.09-present   华东理工大学  生物反应器工程国家重点实验室  副研究员

2011.03-2013.08  华东理工大学  生物反应器工程国家重点实验室 助理研究员

研究方向：

亚胺与胺脱氢酶结构功能与催化机制研究;

2.酶分子改造及其在手性分子合成中的应用研究。

发表论文：

期刊论文：

1. Hao Li, Zheng-Jiao Luan, Gao-Wei Zheng,* and Jian-He Xu*. Efficient Synthesis of Chiral Indolines using an Imine Reductase from Paenibacillus lactis. Advanced Synthesis & Catalysis, 2015, Accepted

2. Zhang Yu-Jun, Zhang Wen-Xia, Gao-Wei Zheng,* and Jian-He Xu*. Identification of an ε-Ketoester Reductase for Efficient Synthesis of (R)-α-Lipoic Acid Precursor. Advanced Synthesis & Catalysis, 2015, DOI 10.1002/adsc.201500001

3. Jin-Gang Yin, Guo-Chao Xu, Gao-Wei Zheng*, Jian-He Xu*. Cloning and characterization of an enantioselective l-menthyl benzoate hydrolase from Acinetobacter sp. ECU2040. Applied Biochemistry and Biotechnology, 2015, Accepted

4.Yu-Cai He*, Dan-Ping Zhang, Zhi-Cheng Tao, Yun Lu, Yun Ding, Feng Liu, Zheng-Zhong Zhu, Huan Rui, Gao-Wei Zheng, Xian Zhang. Improved biosynthesis of ethyl (S)-4-chloro-3-hydroxybutanoate by adding L-glutamine plus glycine instead of NAD+ in beta-cyclodextrin-water system. Bioresource Technology, 2015, 182: 98–102.

5. Yi Gong, Guo-Chao Xu, Gao-Wei Zheng, Chun-Xiu Li, Jian-He Xu*. A thermostable variant of Bacillus subtilis esterase: Characterization and application for resolving dl-menthyl acetate. Journal of Molecular Catalysis B Enzymatic, 2014, 109: 1–8.

6. Rui-Jie Chen, Gao-Wei Zheng*, Yan Ni, Bu-Bing Zeng, Jian-He Xu*. Efficient synthesis of an epsilon-hydroxy ester in a space-time yield of 1580 g L-1 d-1 by a newly identified reductase RhCR. Tetrahedron: Asymmetry, 2014, 25: 1501–1504.

7. Jiang Pan, Ngoc-Duy Dang, Gao-Wei Zheng *, Bin Cheng, Qin Ye, Jian-He Xu*. Efficient production of l-menthol in a two-phase system with SDS using an immobilized Bacillus subtilis esterase. Bioresources and Bioprocessing, 2014, 1: 12

8. Zhi-Jun Zhang, Jing Pan, Chun-Xiu Li, Hui-Lei Yu, Gao-Wei Zheng, Xin Ju, Jian-He Xu*. Efficient production of (R)-(-)-mandelic acid using glutaraldehyde cross-linked Escherichia coli cells expressing Alcaligenes sp. nitrilase. Bioprocess and Biosystems Engineering 2014, 37: 1241–1248.

9. Jia-Yan Liu, Gao-Wei Zheng,* Tadayuki Imanaka, and Jian-He Xu*. Stepwise and combinatorial optimization of enantioselectivity for asymmetric hydrolysis of 1-(3’,4’-methylene dioxyphenyl)ethyl acetate  using a cold-adapted Bacillus amyloliquefaciens esterase.  Biotechnology and Bioprocess Engineering 2014, 19: 442-448.

10.Jing Pan, Gao-Wei Zheng, Qin Ye, Jian-He Xu*. Optimization and scale-up of bioreduction process for cost-effective production of (S)-4-chloro-3-hydroxybutanoate. Organic Process Research and Development, 2014, 18: 739-743

11.Hieu-Huy Nguyen-Tran, Gao-Wei Zheng,* Xu-Hong Qian and Jian-He Xu*. Highly selective and controllable synthesis of arylhydroxylamines by the reduction of nitroarenes with an electron-withdrawing group using a new nitroreductase BaNTR1. Chem Commun 2014, 50, 2861–2864

12.Wei-Jia Zhou, Yan Ni, Gao-Wei Zheng*, Huan-Hui Chen, Zhi-Rong Zhu and Jian-He Xu*. Enzymatic resolution of a chiral chlorohydrin precursor for (R)-lipoic acid synthesis via lipase catalyzed enantioselective transacylation with vinyl acetate. Journal of Molecular Catalysis B-Enzymatic, 2014, 99, 102-107

13.Minh-Thu Ngo-Thi, Jin-Gang Yin, Jiang Pan, Gao-Wei Zheng*, Jian-He Xu*. Enantioselective Hydrolysis of dl-Menthyl Benzoate by Cell-Free Extract of Newly Isolated Acinetobacter sp ECU2040．Applied Biochemistry and Biotechnology, 2013, 170(8): 19741981

14. Dong-Jie Zhou, Jiang Pan, Hui-Lei Yu, Gao-Wei Zheng, Jian-He Xu*. Target-oriented discovery of a new esterase-producing strain Enterobacter sp ECU1107 for whole cell-catalyzed production of (2S,3R)-3-phenylglycidate as a chiral synthon of Taxol. Applied Microbiology and Biotechnology 2013, 97(14): 6293–6300

15. Jia-Yan Liu, Gao-Wei Zheng*, Chun-Xiu Li, Hui-Lei Yu, Jiang Pan, Jian-He Xu*. Multi-substrate fingerprinting of esterolytic enzymes with a group of acetylated alcohols and statistic analysis of substrate spectrum. Journal of Molecular Catalysis B-Enzymatic, 2013, 89: 4147.

16. Gao-Wei Zheng, Xu-Yun Liu, Zhi-Jun Zhang, Ping Tian*, Guo-Qiang Lin and Jian-He Xu*. Separation of enantiopure m-substituted 1-phenylethanols in high space-time yield using Bacillus subtilis esterase. RSC Advvanced 2013, 3: 20446–20449

17.刘佳言、钱乐、郑高伟、许建和。利用结构多样性的对硝基苯酚羧酸酯和脂肪醇乙酸酯评估7个枯草芽孢杆菌酯水解酶的指纹图谱。生物加工过程, 2013, 11(1), 70-76

18. Yan Ni, Jiang Pan, Hong-Min Ma, Chun-Xiu Li, Jie Zhang, Gao-Wei Zheng, Jian-He Xu*. Bioreduction of methyl o-chlorobenzoylformate at 500 g L1 without external cofactors for efficient production of enantiopure clopidogrel intermediate. Tetrahedron Letters, 2012, 53: 4715–4717

19.Chen-Sheng Zhang, Zhi-Jun Zhang, Chun-Xiu Li, Hui-Lei Yu, Gao-Wei Zheng*, Jian-He Xu*. Efficient production of (R)-o-chloromandelic acid by deracemization of o-chloromandelonitrile with a new nitrilase mined from Labrenzia aggregate. Applied Microbiology and Biotechnology 2012, 95: 91–99

20. Hong-Min Ma, Lin-Lin Yang, Yan Ni, Jie Zhang, Chun-Xiu Li, Gao-Wei Zheng, Huai-Yu Yang*, Jian-He Xu*. Stereospecific reduction of methyl o-chlorobenzoylformate at 300 g/L without additional cofactor using a carbonyl reductase mined from Candida glabrata. Advanced Synthesis & Catalysis 2012, 354: 1765–1772

21. Nai-Dong Shen, Yan Ni, Hong-Min Ma, Li-Juan Wang, Chun-Xiu Li, Gao-Wei Zheng, Jie Zhang, Jian-He Xu. Efficient synthesis of a chiral precursor for angiotensin-converting enzyme (ACE) inhibitors in high space-time yield by a new reductase without external cofactors. Organic Letters 2012, 14: 1982–1985

22.Gao-Wei Zheng and Jian-He Xu*. New opportunities for biocatalysis: Driving the   synthesis of chiral chemicals. Current Opinion in Biotechnology, 2011, 22: 784–792

23.Xin Tian, Gao-Wei Zheng*, Chun-Xiu Li, Zhi-Long Wang, Jian-He Xu. Enantioselective production of (S)-1-phenyl-1,2-ethanediol from dicarboxyesters by recombinant Bacillus subtilis esterase. Journal of Molecular Catalysis B: Enzymatic 2011, 73 (1-4): 80–84

24.Gao-Wei Zheng, Hui-Lei Yu, Chun-Xiu Li, Jiang Pan, Jian-He Xu. Immobilization of Bacillus subtilis esterase by simple cross-linking for enzymatic resolution of dl-menthyl acetate. Journal of Molecular Catalysis B: Enzymatic 2011, 70: 138143

25.Gao-Wei Zheng, Jiang Pan, Hui-Lei Yu, Minh-Thu Ngo-Thi, Chun-Xiu Li, Jian-He Xu. An efficient bioprocess for enzymatic production of l-menthol with high ratio of substrate to catalyst using whole cells of recombinant E. coli. Journal of Biotechnology 2010, 150 (1): 108114

26.Gao-Wei Zheng, Hui-Lei Yu, Jian-Dong Zhang, Jian-He Xu*. Enzymatic production of l-menthol by a high substrate concentration tolerable esterase from newly isolated Bacillus subtilis ECU0554. Advanced Synthesis & Catalysis, 2009, 351: 405−414

会议论文：

专著

27.Hui-Lei Yu, Gao-Wei Zheng, Jian-He Xu*. Production of l-menthol by a newly isolated Bacillus subtilis ECU0554 at a high substrate concentration. Abstracts of Papers of the American Chemical Society, 2009, 238: 117-BIOT

28.Gao-Wei Zheng, Jian-Dong Zhang, Jian-He Xu. Enzymatic production of l-menthol by a high substrate concentration tolerable esterase from newly isolated Bacillus subtilis ECU0554. IBS2008 Abstract, Journal of Biotechnology, 2008, 136: 366

研究成果：

近年主持的科研项目：

新型胺脱氢酶催化酮不对称还原胺化的分子机制探索    国家自然科学基金面上项目/ 21472045    2015.01 2018.12

新型微生物亚胺还原酶的催化性能及催化机制研究     国家自然科学基金青年项目/31200050      2013.01 2015.12

手性环氧丙酸(酯)的化学-酶法合成技术     863计划子课题 2011AA02A210     2011.09 2015.12

阿托伐他汀手性中间体生物制造过程的构建    973计划子课题2011CB710806    2011.01 2015.08

基于结构-功能关系的亚胺还原酶分子改造及应用研究    华东理工大学基本科研业务费专项基金22A201514043    2015.03 2017.02

申请专利：

1. 许建和、郑高伟、潘江、郁慧蕾. 枯草芽孢杆菌酯酶及其用于生产l-薄荷醇的应用, 2010.09，中国，ZL200810040988.9；授权日：2010-09-08

2. 许建和，沈乃东，郑高伟，钱小龙，张杰，李春秀，潘江. 一种羰基还原酶及其基因和在不对称还原羰基化合物中的应用，中国，ZL 201110387573.0; 授权日：2013.06.26

3. 马宏敏，李春秀，潘江，郑高伟，郁惠蕾，许建和. 一种羰基还原酶、基因和突变体及在不对称还原羰基化合物中的应用，中国，ZL 201210136823.8; 授权日：2014.06.11

4.许建和，倪燕，潘江，李春秀，马宏敏，郑高伟，郁惠蕾. 生物催化不对称还原制备(R)-邻氯扁桃酸甲酯的方法，申请日：2012.04.11，国际PCT专利申请，申请号：PCT/CN2012/073812; 申请日：2012.04.11

5. 许建和、陈睿杰、郑高伟、潘江、张玉军. 一株红球菌及其用于制备光学纯(R)-6-羟基-8-氯辛酸乙酯及其他光学活性手性醇的用途，中国，申请号：201310236252.X, 申请日：2013.06.17：2014.06.11

6.许建和、张玉军、郑高伟、潘江. 一种假丝酵母羰基还原酶及用于制备(R)-硫辛酸前体的方法，申请号：PCT/CN2015/073615, 申请日：2015.03.04

人才奖项：

华东理工大学青年教学科研骨干