欢迎来到华东理工大学生物反应器工程国家重点实验室!
实验室概况
王华磊
发布时间：2019-07-22 18:07:00
王华磊，理学博士，华东理工大学生物工程学院副教授，硕士生导师。2004年和2007年于中国海洋大学分别获得学士和硕士学位，2013年华东理工大学生物化学与分子生物学专业博士毕业。主要研究方向为生物催化剂——酶的高效开发、人工定制及其在手性药物中间体合成中的应用。2010年11月参与完成的“工业酶制剂的创制与应用关键技术”获上海市科技进步一等奖；2013年12月参与完成的“定向转化多元醇的细胞催化剂创制及其应用关键技术”获上海市技术发明一等奖。作为项目负责人主持国家自然科学基金面上项目、国家自然科学基金青年基金、中国博士后科学基金、华东理工大学基本科研业务费等4个纵向项目，并与永农生物科学有限公司、山东佰安瑞生物药业有限公司、枣庄市杰诺生物酶有限公司等合作开发横向项目3个，作为项目负责人承担总经费1300多万元，参与多项企业横向课题。在Applied and Environmental Microbiology，Microbial Cell Factories，Applied Microbiology and Biotechnology等学术期刊发表SCI论文二十余篇，申请中国发明专利8项，已授权4项。
主要研究方向：
（1）以酶资源多样性、酶催化机理、理性设计方法学为研究内容的生物催化剂高效创制。
（2）以“高值化”产品为导向、基于逆合成生物催化技术的手性药物中间体绿色高效合成工艺开发。
参与或承担的项目：
1、国家自然科学基金面上项目，21776084， 2018/01-2021/12，71万元，主持。
2、国家自然科学基金青年基金，21406068，2015/01-2017/12，25万元，主持。
3、中国博士后科学基金面上一等资助，2014M560308， 2014/01-2015/12，8万元，主持。
4、华东理工大学基本科研业务费探索研究基金，222201514324，2016/01-2017/12，20万元，主持。
5、永农生物科学有限公司（横向课题），2017/01-2019/12，1200万元，主持。
6、山东佰安瑞生物药业有限公司（横向课题），2014/09-2018/08，30万元，主持。
7、枣庄市杰诺生物酶有限公司（横向课题），2015/09-2020/12，20万元，主持。
8、国家自然科学基金面上项目，21676069， 2017/01-2020/12，70万元，项目骨干。
9、国家自然科学基金青年基金，21606062， 2017/01-2019/12，20万元，项目骨干。
发表论文（*通讯作者）：
1. Haiyang Fan, Lifeng Chen, Huihui Sun, Hualei Wang*, Qinghai Liu, Yuhong Ren, Dongzhi Wei. Characterization of a novel nitrilase, BGC4, from Paraburkholderia graminis showing wide-spectrum substrate specificity, a potential versatile biocatalyst for the degradation of nitriles. Biotechnology Letters, 2017, 39(11): 1725–1731.
2. Haiyang Fan, Lifeng Chen, Huihui Sun, Hualei Wang*, Qinghai Liu, Yuhong Ren, Dongzhi Wei. Development of nitrilase-mediated process for phenylacetic acid production from phenylacetonitrile. Chemical Papers, 2017, 71(10): 1985–1992.
3. Haiyang Fan, Lifeng Chen, Huihui Sun, Hualei Wang*, Yuhong Ren, Dongzhi Wei. A novel nitrilase from Ralstonia eutropha H16 and its application to nicotinic acid production. Bioprocess and Biosystems Engineering, 2017, 40(8):1271-1281.
4. Lifeng Chen, Haiyang Fan, Yiping Zhang, Wei Wei, Jinping Lin, Dongzhi Wei, Hualei Wang*. Enhancement of ethyl (S)-4-chloro-3-hydroxybutanoate production at high substrate concentration by in situ resin adsorption. Journal of biotechnology, 2017, 251:68-75.
5. Lifeng Chen, Yiping Zhang, Haiyang Fan, Kai Wu, Jinping Lin, Hualei Wang*, Dongzhi Wei. Efficient bioreductive production of (R)-N-Boc-3-hydroxypiperidine by a carbonyl reductase. Catalysis Communications, 2017, 97:5-9.
6. Lifeng Chen, Haiyang Fan, Yiping Zhang, Kai Wu, Hualei Wang*, Jinping Lin, Dongzhi Wei. Development of a practical biocatalytic process for (S)-N-Boc-3-hydroxypiperidine synthesis. Tetrahedron Letters, 2017, 58(16):1644–1650.
7. Yiping Zhang, Hualei Wang*, Lifeng Chen, Kai Wu, Jingli Xie, Dongzhi Wei. Efficient production of ethyl (R)-4-chloro-3-hydroxybutanoate by a novel alcohol dehydrogenase from Lactobacillus curieae S1L19. Journal of Molecular Catalysis B: Enzymatic. 2016, 134:51-60
8. Wenyuan Gao, Haiyang Fan, Lifeng Chen, Hualei Wang*, Dongzhi Wei. Efficient kinetic resolution of secondary alcohols using an organic solvent-tolerant esterase in non-aqueous medium. Biotechnology Letters. 2016, 38(7):1165-1171
9. Kai Wu, Hualei Wang#*, Lifeng Chen, Haiyang Fan, Zhiqiang Zhao, Dongzhi Wei*. Practical two-step synthesis of enantiopure styrene oxide through an optimized chemoenzymatic approach. Applied Microbiology and Biotechnology. 2016, 100(20): 8757-8767
10. Zhiqiang Zhao, Hualei Wang*, Yiping Zhang, Lifeng Chen, Kai Wu, Dongzhi Wei. Cloning and characterization of three ketoreductases from soil metagenome for preparing optically active alcohols. Biotechnology Letters. 2016, 38(10):1799-1808
11. Wenyuan Gao, Kai Wu, Lifeng Chen, Haiyang Fan, Zhiqiang Zhao, Bei Gao, Hualei Wang*, Dongzhi Wei*. A novel esterase from a marine mud metagenomic library for biocatalytic synthesis of short-chain flavor esters. Microbial Cell Factories, 2016, 15:41.
12. Kai Wu, Lifeng Chen, Haiyang Fan, Zhiqiang Zhao, Hualei Wang*, Dongzhi Wei*. Synthesis of enantiopure epoxide by ‘one pot’ chemoenzymatic approach using a highly enantioselective dehydrogenase. Tetrahedron Letters, 2016, 57(8):899-904.
13. Huihui Sun, Wenyuan Gao, Hualei Wang*, Dongzhi Wei. Expression, characterization of a novel nitrilase PpL19 from Pseudomonas psychrotolerans with S-selectivity toward mandelonitrile present in active inclusion bodies. Biotechnology letters, 2016，38(3):455-461
14. Hualei Wang, Haiyang Fan, Huihui Sun, Li Zhao, Dongzhi Wei*. Process development for production of (R)-(-)-mandelic acid by recombinant Escherichia coli cells harboring nitrilase from Burkholderia cenocepacia J2315. Organic Process Research & Development, 2015,19(12),2010-2016
15. Hualei Wang, Wenyuan Gao, Huihui Sun, Lifeng Chen, Lujia Zhang, Xuedong Wang*, Dongzhi Wei*. Protein engineering of nitrilase from Burkholderia cenocepacia J2315 for efficient and enantioselective production of (R)-o-chloromandelic acid. Applied and Environmental Microbiology, 2015, 81(24):8469-8477.
16. Huihui Sun, Hualei Wang*, Wenyuan Gao, Lifeng Chen, Kai Wu, Dongzhi Wei*. Directed evolution of nitrilase PpL19 from Pseudomonas psychrotolerans L19 and identification of enantiocomplementary mutants toward mandelonitrile. Biochemical and Biophysical Research Communications, 2015, 468(4):820-825.
17. Huihui Sun, Wenyuan Gao, Haiyang Fan, Hualei Wang*, Dongzhi Wei. Cloning, purification and evaluation of the enzymatic properties of a novel arylacetonitrilase from Luminiphilus syltensis NOR5-1B: a potential biocatalyst for the synthesis of mandelic acid and its derivatives. Biotechnology letters, 2015，37(8):1655–1661.
18. Kai Wu, Hualei Wang*, Huihui Sun, Dongzhi Wei*. Efficient kinetic resolution of phenyl glycidyl ether by a novel epoxide hydrolase from Tsukamurella paurometabola. Applied Microbiology and Biotechnology, 2015, 99(22):9511-9521
19. Hualei Wang, Huihui Sun, Wenyuan Gao, Dongzhi Wei*. Efficient production of (R)-o-chloromandelic acid by recombinant Escherichia coli cells harboring nitrilase from Burkholderia cenocepacia J2315. Organic Process Research & Development, 2014, 18(6):767–773.
20. Hualei Wang, Guinan Li, Mingyang Li, Dongzhi Wei*, Xuedong Wang*. A novel nitrilase from Rhodobacter sphaeroides LHS-305-cloning, heterologous expression and biochemical characterization. World Journal of Microbiology and Biotechnology, 2014, 30(1):245-252.
21. Hualei Wang, Huihui Sun, Dongzhi Wei*. Discovery and characterization of a highly efficient enantioselective mandelonitrile hydrolase from Burkholderia cenocepacia J2315 by phylogeny-based enzymatic substrate specificity prediction．BMC Biotechnology, 2013, 13:14.
22. Kefeng Ni, Hualei Wang（并列第一作者）, Li Zhao, Minjie Zhang, Siyuan Zhang, Yuhong Ren*, Dongzhi Wei*. Efficient production of (R)-(-)-mandelic acid in biphasic system by immobilized recombinant E.coli. Journal of Biotechnology, 2013, 167(4):433-440.
23. Shuiqin Jiang, Lujia Zhang*, Zhiqiang Yao, Bei Gao, Hualei Wang, Xiangzhao Mao, Dongzhi Wei*. Switching a nitrilase from Syechocystis sp. PCC6803 to a nitrile hydratase by rationally regulating reaction pathways. Catalysis Science & Technology, 2017, 7, 1122-1128.
24. Jian Qiu, Erzheng Su*, Hualei Wang, Wenwen Cai, Wei Wang, Dongzhi Wei*. Cloning, overexpression, and characterization of a high enantioselective nitrilase from Sphingomonas wittichii RW1 for asymmetric synthesis of (R)-phenylglycine. Applied Biochemistry and Biotechnology, 2014, 173(2):365-377.
25. Lujia Zhang, Bo Yin, Chao Wang, Shuiqin Jiang, Hualei Wang, Dongzhi Wei*, Y Adam Yuan*. Structural insights into enzymatic activity and substrate specificity determination by a single amino acid in nitrilase from Syechocystis sp. PCC6803. Journal of Structural Biology, 2014, 188(2):93-101.
26. Kefeng Ni, Xu Zhou, Li Zhao, Hualei Wang, Yuhong Ren, Dongzhi Wei*. Magnetic Catechol-Chitosan with Bioinspired Adhesive Surface: Preparation and Immobilization of omega-Transaminase. Plos One, 2012, 7(7): e41101
专利：
1、 魏东芝、许向阳、宋在伟、王华磊。一种生物法制备苯乙酸的方法。中国发明专利，申请号：201611110169.8。
2、 魏东芝、许向阳、宋在伟、王华磊。一种生物法合成(R)‑邻氯扁桃酸的方法。中国发明专利，申请号：201611110170.0。
3、 王华磊、谢有余、王继国、范海洋、魏东芝。一种S型的ω-转氨酶ATA-W12及其基因和应用，中国发明专利，申请号：201611055835.2。
4、 王华磊、张一平、魏东芝。一种醇脱氢酶LC3及其基因和应用，中国发明专利，申请号：201610066375.7。
5、 王华磊、范海洋、任宇红、魏东芝。一种生物法合成(R)-(-)-扁桃酸的方法，中国发明专利，申请号：201510334809.2。
6、 王华磊、高文渊、魏东芝。酯酶est4、酯酶EST4、重组质粒和基因工程菌株及其应用，中国发明专利，授权号：ZL 201510342723.4。
7、 王华磊、陈利锋、魏东芝。一种不对称转化制备(S)-4-氯-3-羟基丁酸乙酯的重组大肠杆菌及其应用，中国发明专利，申请号：201510098687.1。
8、 王华磊、吴锴、魏东芝。一种环氧化物水解酶及其编码基因和应用，中国发明专利，授权号：ZL 201410175055.8。
联系方式：
上海市梅陇路130号311信箱 邮编:200237
办公室：实验十八楼707室
电话：021-64251803
E-mail address: hlwang@ecust.edu.cn