张元兴

1.    周祥山，博士，教授，博士生导师，中组部“万人计划”第一批科技创新领军人才，上海市科技启明星，山东省泰山学者。参加国家自然科学基金项目，主要从事微生物发酵和生物制药的研究。

2.   刘琴，博士，教授，博士生导师，国家优秀青年基金获得者，上海市科技启明星，教育部新世纪人才，任生物反应器工程国家重点实验室副主任，阿华生物工程研究所所长，上海海洋动物疫苗工程技术研究中心主任。承担国家重点研发计划项目、国家自然科学基金项目，主要从事海洋生物技术研究。

3.   王启要，博士，教授，博士生导师，上海市科技启明星和浦江学者，任生物工程学院副院长，上海海洋动物疫苗工程技术研究中心副主任。国家鲆鲽类农业技术体系疾病防控岗位科学家。承担国家自然科学基金项目和上海市自然科学基金项目，参加国家重点研发计划课题，主要从事海洋生物技术研究。

4.   蔡孟浩，博士，副教授，博士生导师，上海市科技启明星，任生物工程学院院长助理，上海海洋动物疫苗工程技术研究中心副主任。承担国家重点研发计划课题、国家自然科学基金和上海市自然科学基金项目，主要从事海洋微生物和合成生物学的研究。

5.   阳大海，博士，副教授，硕士生导师，上海市浦江学者和晨光学者，入选中国科协青年人才托举计划。承担国家自然科学基金项目，参加国家重点研发计划课题，主要从事海洋生物技术研究。

6.   马悦，博士，副教授，硕士生导师。承担国家自然科学基金项目，参加国家重点研发计划课题和国家鲆鲽类农业技术体系项目，主要从事海洋生物技术研究。

7.   刘晓红，博士，副教授，硕士生导师。承担国家自然科学基金项目，参加国家重点研发计划课题，主要从事海洋动物疫苗的研究。

8.   殷开宇，博士后。承担扬帆计划计划项目和中国博士后科学基金项目，参加国家自然科学基金项目，主要从事海洋生物技术的研究。

9.   陈守文，博士后。承担国家自然科学基金项目和中国博士后科学基金项目，主要从事海洋生物技术的研究。

10.   邵帅，博士后。承担国家自然科学基金项目、扬帆计划计划项目和中国博士后科学基金项目，主要从事海洋生物技术的研究。

11.   张艺蓓，博士后，

12.   陆健，学士，高级工程师。主要从事生物反应器的研究和设计。

13.   林雅惠，学士。实验研究和实验室管理工作。

主要的研究方面：

1. 海洋生物技术。研究海洋病原微生物的特性，掌握其致病规律，在分子水平上阐明细胞通讯相关的条件致病现象，研究病原细菌和宿主之间的互作机制，从基因水平上进行改造，开发减毒活疫苗和多价疫苗。目前承担着国家自然科学基金重点和面上项目、现代农业产业技术体系岗位科学家项目。

2. 微生物工程。研究甲醇酵母基因诱导水平的精密控制网络，构建新的蛋白表达体系。解析生物合成代谢途径和相关基因（簇），在毕赤酵母中重构代谢网络，高效异源生物合成小分子活性化合物。在海洋微生物培养中，阐明丝状菌次级代谢调控的规律，调控菌丝形态，高效合成活性化合物。目前承担着国家自然科学基金项目和企业委托项目。

3. 动物细胞培养。研究动物细胞的生理和代谢规律，开发无血清培养基，建立动物细胞培养生产蛋白药物的优化工艺。研究动物细胞表达外源基因的高效体系，构建适合于大规模无血清培养的重组细胞株。目前承担着企业委托项目。

著作：

1.   王启要，刘琴，张元兴 (2008) 群体感应：细菌之间的语言. 叶勤主编：生物科学与工程. 北京：科学出版社. p43-67.

2.    张元兴 (2008) 动物细胞培养工程. 叶勤主编：生物科学与工程. 北京：科学出版社. p160-171.

3.    张元兴，易小萍，张立，孙祥明 (2007) 动物细胞培养工程. 北京：化工出版社.

4.    张元兴，许学书 (2001) 生物反应器工程. 上海：华东理工大学出版社.

2020年发表和出版中论文:

1.         Kaiyu Yin, Jin Zhang, Jiabao Ma, Peng Jin, Yue Ma, Yuanxing Zhang, Xiaohong Liu*, Qiyao Wang*. MviN mediates the regulation of environmental osmotic pressure on esrB to control the virulence in the marine pathogen Edwardsiella piscicida. Microbiological Research 2020, 239: 126528.

2.         Jiao Wen, Lin Tian, Qi Liu, Yuanxing Zhang, Menghao Cai*. Engineered dynamic distribution of malonyl-CoA flux for improving polyketide biosynthesis in Komagataella phaffii. Journal of Biotechnology 2020, 320: 80~85.

3.         Chenxiao Bai, Yiqi Liu, Xinjie Chen, Zhilan Qian, Haifeng Liu, Xiangshan Zhou, Yuanixng Zhang, Menghao Cai*. Fungal statin pump protein improves monacolin J efflux and regulates its production in Komagataella phaffii. Bioresources and Bioprocessing 2020, 7: 32.

4.         Yu Han, Lifan Wei, Jingfan Xiao, Yuanxing Zhang, Qiyao Wang*, Mian Zhou*. Identification and study of InV as an inverse autotransporter family representative in Edwardsiella piscicida. Archives of Microbiology 2020, 202(5): 1107~1116.

5.         匡露寒, 季国荣, 杨灿灿, 张元兴, 刘琴*. 基于抗原蛋白Fiber-2及细胞因子佐剂的Ⅰ群4型禽腺病毒亚单位疫苗的开发. 中国兽医科学2020, 50(4): 452~458.

6.         Hao Chen, Shuangfei Ding, Jinchao Tan, Dahai Yang, Yuanxing Zhang, Qin Liu*. Characterization of the Japanese flounder NLRP3 inflammasome in restricting Edwardsiella piscicida colonization in vivo. Fish & Shellfish Immunology 2020, 103: 169~180.

7.         Jiao Wen, Lin Tian, Mingqiang Xu, Xiangshan Zhou, Yuanxing Zhang, Menghao Cai*. A synthetic malonyl-CoA metabolic oscillator in Komagataella phaffii. ACS Synthetic Biology 2020, 9(5): 1059~1068.

8.         Lei Shi, Jinjia Wang, Xiaolong Wang, Yuanxing Zhang, Zhiwei Song, Menghao Cai, Xiangshan Zhou*. Transcriptional regulatory networks of methanol-independent protein expression in Pichia pastoris under the AOX1 promoter with trans-acting elements engineering. Bioresources and Bioprocessing 2020, 7: 18.

9.         Yanna Ren, Qi Liu, Haifeng Liu, Xiangshan Zhou, Yuanxing Zhang, Menghao Cai*. Engineering substrate and energy metabolism for living cell production of cytidine-5’-diphosphocholine. Biotechnology and Bioengineering 2020, 117(5): 1426~1435.

10.     Zhuang Wang, Zhaoyan Gu, Qing Hou, Weijie Chen, Di Mu, Yuanxing Zhang, Qin Liu, Zhihong Liu, Dahai Yang*. Zebrafish GSDMEb cleavage-gated pyroptosis drives septic-acute kidney injury in vivo. The Journal of Immunology 2020, 204(7): 1929~1942.

11.     Wenhui Wang#, Jinchao Tan#, Zhuang Wang, Yuanxing Zhang, Qin Liu, Dahai Yang*. Characterization of the inflammasome component SmASC in turbot (Scophthalmus maximus). Fish & Shellfish Immunology 2020, 100: 324~333.

12.     Xiang Sun, Peng Jin, Qin Liu, Qiyao Wang, Yuanxing Zhang, Xiaohong Liu*. A CpG-riched plasmid as vaccine adjuvant reduce antigen dose of an inactivated Vibro anguillarum vaccine in turbot (Scophthalmus maximus L.). Fish & Shellfish Immunology 2020, 98: 312-317.

13.     Kaiyu Yin, Yue Peng, Moamer A.H. Ahmed, Jiabao Ma, Rongjing Xu, Yuanxing Zhang, Yue Ma*, Qiyao Wang*. PepA binds to and negatively regulates esrB to control virulence in the fish pathogen Edwardsiella piscicida. Microbiological Research 2020, 232: 126349.

2019年以前发表的主要文章:

1.         Lifan Wei, Haoxian Qiao, Brandon Sit, Kaiyu Yin, Guanhua Yang, Ruiqing Ma, Jiabao Ma, Chun Yang, Jun Yao, Yue Ma, Jingfan Xiao, Xiaohong Liu, Yuanxing Zhang, Matthew K. Waldor, Qiyao Wang*. A bacterial pathogen senses host mannose to coordinate virulence. iScience 2019, 20: 310~323.

2.         Dahai Yang, Xiaohong Liu, Wenting Xu, Zhaoyan Gu, Cuiting Yang, Lingzhi Zhang, Jinchao Tan, Xin Zheng, Zhuang Wang, Shu Quan, Yuanxing Zhang, Qin Liu*. The Edwardsiella piscicida thioredoxin-like protein inhibits ASK1-MAPKs signaling cascades to promote pathogenesis during infection. PLoS Pathogens 2019, 15(7): e1007917.

3.         Dan Gu, Jun Zhang, Yuan Hao, Rongjing Xu, Yuanxing Zhang, Yue Ma*, Qiyao Wang*. Alternative sigma factor RpoX is a part of RpoE regulon and plays distinct roles in stress response, motility, biofilm formation and hemolytic activities in the marine pathogen Vibrio alginolyticus. Applied and Environmental Microbiology 2019, 85(14): e00234-19.

4.         Ying Wen, Shouwen Chen, Zhiwei Jiang, Zhuang Wang, Jinchao Tan, Tianjian Hu, Qiyao Wang, Xiangshan Zhou, Yuanxing Zhang, Qin Liu, Dahai Yang*. Dysregulated hemolysin promotes bacterial outer membrane vesicles-induced pyroptotic-like cell death in zebrafish. Cellular Microbiology 2019, 21(6): e13010.

5.         Yiqi Liu, Chenxiao Bai, Qi Liu, Qin Xu, Zhilan Qian, Qiangqiang Peng, Jiahui Yu, Mingqiang Xu, Xiangshan Zhou, Yuanxing Zhang, Menghao Cai*. Engineered ethanol-driven biosynthetic system for improveing production of acetyl-CoA derived drugs in Crabtree-negative yeast. Metabolic Engineering 2019, 54: 275-284.

6.         Lifan Wei, Yanyan Wu, Guanhua Yang, Rongjing Xu, Xiaohong Liu, Qin Liu, Yuanxing Zhang, Yue Ma*, Qiyao Wang*. Genome-wide identification of fitness factors in seawater for Edwardsiella piscicida. Applied and Environmental Microbiology 2019, 85(10): e00233-19.

7.         Qin Xu, Chenxiao Bai, Yiqi Liu, Lili Song, Lin Tian, Yunfeng Yan, Jinfeng Zhou, Xiangshan Zhou, Yuanxing Zhang, Menghao Cai*. Modulation of acetate utilization in Komagataella phaffii by metabolic engineering of tolerance and metabolism. Biotechnology for Biofuels 2019, 12: 61.

8.         Kaiyu Yin, Yunpeng Guan, Ruiqing Ma, Lifan Wei, Bing Liu, Xiaohong Liu, Xiangshan Zhou, Yue Ma, Yuanxing Zhang, Matthew K. Waldor, Qiyao Wang*. Critical role for a promoter discriminator in RpoS control of virulence in Edwardsiella piscicida. PLoS Pathogens 2018, 14(8): e1007272.

9.         Shouwen Chen, Dahai Yang, Ying Wen, Zhiwei Jiang, Lingzhi Zhang, Jiatiao Jiang, Yaozhen Chen, Tianjian Hu, Qiyao Wang, Yuanxing Zhang, Qin Liu*. Dysregulated hemolysin liberates bacterial outer membrane vesicles for cytosolic lipopolysaccharide sensing. PLoS Pathogens 2018, 14(8): e1007240.

10.     Dahai Yang, Xin Zheng, Shouwen Chen, Zhuang Wang, Wenting Xu, Jinchao Tan, Tianjian Hu, Mingyu Hou, Wenhui Wang, Zhaoyan Gu, Qiyao Wang, Ruilin Zhang, Yuanxing Zhang, Qin Liu*. Sensing of cytosolic LPS through caspy2 pyrin domain mediates noncanonical inflammasome activation in zebrafish. Nature Communications 2018, 9: 3052.

11.     Xiating Gao, Xuetong Wang, Qiaoqiao Mao, Rongjing Xu, Xiaohui Zhou, Yue Ma, Qin Liu, Yuanxing Zhang, Qiyao Wang*. VqsA, a novel LysR-type transcriptional regulator coordinates quorum sensing (QS) and is controlled by QS to regulate virulence in the pathogen Vibrio alginolyticus. Applied and Environmental Microbiology 2018, 84(12): e00444-18.

12.     Zhen Yang, Xiaohui Zhou, Yue Ma, Mian Zhou, Matthew K. Waldor, Yuanxing Zhang, Qiyao Wang*. Serine/threonine kinase PpkA coordinates the interplay between T6SS2 activation and quorum sensing in the marine pathogen Vibrio alginolyticus. Environmental Microbiology 2018, 20(2): 903-909.

13.     Yiqi Liu, Xiaohu Tu, Qin Xu, Chenxiao Bai, Chuixing Kong, Qi Liu, Jiahui Yu, Qiangqiang Peng, Xiangshan Zhou, Yuanxing Zhang, Menghao Cai*. Engineering monoculture and co-culture of methylotrophic yeast for de novo production of monacolin J and lovastatin from methanol. Metabolic Engineering 2018, 45: 189-199.

14.     Huifang Cao, Cuiting Yang, Shu Quan, Tianjian Hu, Lingzhi Zhang, Yuanxing Zhang, Dahai Yang*, Qin Liu*. Novel T3SS effector EseK in Edwardsiella piscicida is chaperoned by EscH and EscS to express virulence. Cellular Microbiology 2018, 20(1): e12790.

15.     Yuan Wu, Yanna Ren, Xiangshan Zhou, Menghao Cai*, Yuanxing Zhang*. Transcription factor Agseb1 affects development, osmotic stress response, and secondary metabolism in marine-derived Aspergillus glaucus. Journal of Basic Microbiology 2017, 57(10): 873-882.

16.     Guanhua Yang, Gabriel Billings, Troy Hubbard, Joseph Park, Ka Yin Leung, Qin Liu, Brigid M. Davis, Yuanxing Zhang, Qiyao Wang*, Matthew K. Waldor. Time resolved transposon insertion sequencing reveals genome-wide fitness dynamics during infection. mBio 2017, 8(5): e01581-17.

17.     Yue Ma, Qiyao Wang*, Wensheng Xu, Xiaohong Liu, Xiating Gao, Yuanxing Zhang*. Stationary phase-dependent accumulation of ectoine is an efficient adaptation strategy in Vibrio anguillarum against cold stress. Microbiological Research 2017, 205: 8-18.

18.     Ying Yin, Qiang Fu, Wenhui Wu, Menghao Cai, Xiangshan Zhou, Yuanxing Zhang*. Producing novel fibrinolytic isoindolinone derivatives in marine fungus Stachybotrys longispora FG216 by rational amino precursors supply according to its biosynthesis pathway. Marine Drugs 2017, 15(7): 214.

19.     Hao Chen, Dahai Yang, Fajun Han, Jinchao Tan, Lingzhi Zhang, Jingfan Xiao, Yuanxing Zhang, Qin Liu*. The bacterial T6SS effector EvpP prevents NLRP3 inflammasome activation by inhibiting Ca²⁺-dependent MAPK-Jnk pathway. Cell Host & Microbe 2017, 21(1): 47-58.

20.     Yue Ma, Qiyao Wang, Xiating Gao, Yuanxing Zhang*. Biosynthesis and uptake of glycine betaine as cold-stress response to low temperature in fish pathogen Vibrio anguillarum. Journal of Microbiology 2017, 55(1): 44-55.

21.     Ying Yin*, Menghao Cai, Xiangshan Zhou, Zhiyong Li, Yuanxing Zhang*. Polyketides in Aspergillus terreus: biosynthesis pathway discovery and application. Applied Microbiology and Biotechnology 2016, 100(18): 7787-7798.

22.     Dan Gu, Min Guo, Minjun Yang, Yuanxing Zhang, Xiaohui Zhou*, Qiyao Wang*. A s^E-mediated temperature gauge controls a switch from LuxR-mediated virulence gene expression to thermal stress adaptation in Vibrio alginolyticus. PLoS Pathogens 2016, 12(6): e1005645.

23.     Xiaolong Wang, Qi Wang, Jinjia Wang, Peng Bai, Lei Shi, Wei Shen, Mian Zhou, Xiangshan Zhou, Yuanxing Zhang, Menghao Cai*. Mit1 transcription factor mediates methanol signaling and regulates alcohol oxidase 1 promoter in Pichia pastoris. Journal of Biological Chemistry 2016, 291(12): 6245-6261.

24.     Tongcheng Dai, Na Li, Fajun Han, Hua Zhang, Yuanxing Zhang, Qin Liu*. AMP-guided tumour-specific nanoparticle delivery via adenosine A1 receptor. Biomaterials 2016, 83: 37-50.

25.     John S. Y. Goh, Yingwei Liu, Haifeng Liu, Kah Fai Chan, Corrine Wan, Gavin Teo, Xiangshan Zhou, Fusheng Xie, Peiqing Zhang, Yuanxing Zhang*, Zhiwei Song* (2014) Highly sialylated recombinant human erythropoietin production in large-scale perfusion bioreactor utilizing CHO-gmt4 (JW152) with restored GnT I function. Biotechnology Journal 9(1): 100-109.

26.     Pham Ngoc Tu, Yamin Wang, Menghao Cai, Xiangshan Zhou*, Yuanxing Zhang* (2014) Co-expressions of protein tyrosine kinases EGFR-2 and PDGFRβ with protein tyrosine phosphatase 1B in Pichia pastoris. Journal of Microbiology and Biotechnology 24(2): 152-159.

27.     Yingwei Liu, Xiangshan Zhou, Zhiwei Song*, Yuanxing Zhang* (2014) Sodium butyrate enhances the acidic isoform content of recombinant human erythropoietin produced by Chinese hamster ovary cells. Biotechnology Letters 36(5): 907-911.

28.     Haifeng Liu, Xiangshan Zhou*, Shousheng Tian, Xianghui Hao, Jinhua You, Yuanxing Zhang* (2014) Two-step transpeptidation of the insulin precursor expressed in Pichia pastoris to insulin ester via trypsin-catalyzed cleavage and coupling. Biotechnology and Applied Biochemistry 61(4): 408-417.

29.     Lili Sheng, Yuanzhi Lv, Qin Liu,Qiyao Wang*,Yuanxing Zhang* (2013) Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Veterinary Microbiology 162(2-4): 652-662.

30.     Haifeng Liu, Xiangshan Zhou*, Fusheng Xie, Jinhua You,Yuanxing Zhang* (2013) An efficient trypsin digestion strategy for improving desB30 productivity from recombinant human insulin precursor fusion protein. Process Biochemistry 48(5-6): 965-971.

31.     Huan Liu, Dan Gu, Xiaodan Cao, Qin Liu,Qiyao Wang*,Yuanxing Zhang* (2012) Characterization of a new quorum sensing regulator luxT and its roles in the extracellular protease production, motility, and virulence in fish pathogen Vibrio alginolyticus. Archives of Microbiology 194(6): 439-452.

32.     Ming Liu, Shuang Wang, Tingguang Sun, Jiguo Su,Yuanxing Zhang*, Junjie Yue*,Zhiwei Sun* (2012) Insight into the structure, dynamics and the unfolding property of amylosucrases: Implications of rational engineering on thermostability. PLoS One 7(7): e40441.

33.     Dahai Yang, Qin Liu*, Mingjun Yang, Haizhen Wu, Qiyao Wang, Jingfan Xiao,Yuanxing Zhang* (2012) RNA-seq liver transcriptome analysis reveals an activated MHC-I pathway and an inhibited MHC-II pathway at the early stage of vaccine immunization in zebrafish. BMC Genomics 13: 319.

34.     Menghao Cai, Xiangshan Zhou*, Jian Lu, Weimin Fan, Jiushun Zhou, Chuanpeng Niu, Li Kang, Xunqian Sun,Yuanxing Zhang* (2012) An integrated control strategy for the fermentation of the marine-derived fungus Aspergillus glaucus for the production of anti-cancer polyketide. Marine Biotechnology 14(6): 665-671.

35.     Menghao Cai, Xueqian Sun, Xiangshan Zhou*,Yuanxing Zhang* (2012) Roles of cobalt in biosynthesis stimulation of a cytotoxic compound from marine-derived Aspergillus glaucus. Process Biochemistry 47(12): 2267-2274.

36.     Menghao Cai, Xiangshan Zhou*, Jian Lu, Weimin Fan, Chuanpeng Niu, Jiushun Zhou, Xueqian Sun, Li Kang,Yuanxing Zhang* (2011) Enhancing aspergiolide A production from a shear-sensitive and easy-foaming marine-derived filamentous fungus Aspergillus glaucus by oxygen carrier addition and impeller combination in a bioreactor. Bioresource Technology 102(3): 3584-3586.

37.     Xiaodan Cao,Qiyao Wang, Qin Liu*, Haopeng Rui, Huan Liu,Yuanxing Zhang* (2011) Identification of a luxO-regulated extracellular protein Pep and its roles in motility in Vibrio alginolyticus. Microbial Pathogenesis 50(2):123-131.

38.     Lu Cheng, Xiangming Sun, Xiaoping Yi,Yuanxing Zhang* (2011) Transient gene expression of a mouse homolog of Fce-Fcg fusion protein for anti-allergic function assay. Process Biochemistry 46(4): 858-863.

39.     XinWang, QiyaoWang*, Minjun Yang, Jingfan Xiao, Qin Liu, Haizhen Wu,Yuanxing Zhang* (2011) QseBC controls flagellar motility, fimbrial hemagglutination and intracellular virulence in fish pathogen Edwardsiella tarda. Fish & Shellfish Immunology 30(3): 944-953.

40.     Honghong He,Qiyao Wang, Lili Sheng, Qin Liu*,Yuanxing Zhang* (2011) Functional characterization of Vibrio alginolyticus twin-arginine translocation system: its roles in stress adaptation and virulence towards fish. Current Microbiology 62(4): 1193-1199.

41.     Yanhua Pan, WeiWei, HongyanXu,Qiyao Wang, Qin Liu, Haizhen Wu*,Yuanxing Zhang* (2011) Differential gene expression in Japanese flounder (Paralichthys olivaceus) induced by live attenuated Vibrio anguillarum. Aquaculture Research 42(7):1042-1049.

42.     Huan Liu,Qiyao Wang*, Qin Liu, Xiaodan Cao, Cunbin Shi,Yuanxing Zhang* (2011) Roles of Hfq in the stress adaptation and virulence in fish pathogen Vibrio alginolyticus and its potential application as a target for live attenuated vaccine. Applied Microbiology and Biotechnology 91(2):353-364.

43.     Lu Cheng, Xiangming Sun, Xiaoping Yi,Yuanxing Zhang* (2011) Large-scale plasmid preparation for transient gene expression. Biotechnology Letters 33(8): 1559-1564.

44.     Yan Zhao, Qin Liu*, Xiuhua Wang, Lingyun Zhou,Qiyao Wang,Yuanxing Zhang* (2011) Surface display of Aeromonas hydrophila GAPDH in attenuated Vibrio anguillarum to develop a novel multivalent vector vaccine. Marine Biotechnology 13(5): 963-970.

45.     Jingfan Xiao, Tao Chen,Qiyao Wang*, Qin Liu, Xin Wang, Yuanzhi Lv, Haizhen Wu,Yuanxing Zhang* (2011) Search for live attenuated vaccine candidate against edwardsiellosis by mutating virulence-related genes of fish pathogen Edwardsiella tarda. Letters in Applied Microbiology 53(4): 430-437.

46.     Xiaonuo Teng, Xiaoping Yi, Xiangming Sun,Yuanxing Zhang* (2011) Modeling and application of controlled-fed perfusion culture of CHO cells in a bioreactor. Chemical and Biochemical Engineering Quarterly 25(3): 385-394.

47.     Wei Wei, Haizhen Wu*,Hongyan Xu, Teng Chu, Changwen Wu, Kangmei Chang,Yuanxing Zhang* (2010) Cloning and molecular characterization of lycC1INH genes in large yellow croaker (Pseudosciaena crocea). Fish & Shellfish Immunology 28(1): 221-227.

48.     Xueqian Sun, Xiangshan Zhou*, Menghao Cai, Jiushun Zhou,Yuanxing Zhang* (2010) Significant stimulation of o-phthalic acid in biosynthesis of aspergiolide A by a marine fungus Aspergillus glaucus. Bioresource Technology 101(10):3609-3616.

49.     Lingyun Zhou, Xiuhua Wang, Qin Liu*,Qiyao Wang, Yan Zhao,Yuanxing Zhang* (2010) A novel multivalent vaccine based on secretary antigen-delivery induces protective immunity against Vibrio anguillarum and Aeromonas hydrophila. Journal of Biotechnology 146(1-2): 25-30.

50.     Haopeng Rui, Jennifer M. Ritchie, Roderick T. Bronson, John J. Mekalanos,Yuanxing Zhang, Matthew K. Waldor* (2010) Reactogenicity of live-attenuated Vibrio cholerae vaccines is dependent on flagellins. Proceedings of theNationalAcademyof Sciences of theUnited States of America107(9):4359-4364.

51.     Xiaodan Cao,Qiyao Wang, Qin Liu*, Huan Liu, Honghong He,Yuanxing Zhang* (2010) Vibrio alginolyticus MviN is a LuxO-regulated protein and affects cytotoxicity toward EPC cell. Journal of Microbiology and Biotechnology 20(2): 271-280.

52.     Xiao Zhang, Wei Wei, Haizhen Wu*,Hongyan Xu, Kangmei Chang,Yuanxing Zhang* (2010) Gene cloning and characterization of ferritin H and M subunits from large yellow croaker (Pseudosciaena crocea). Fish & Shellfish Immunology 28(5-6):735-742.

53.     Menghao Cai, Xiangshan Zhou*, Jiushun Zhou, Chuanpeng Niu, Xueqian Sun,Yuanxing Zhang* (2010) Efficient strategy for enhancing aspergiolide A production by citrate feedings and its effects on sexual development and growth of marine-derived fungus Aspergillus glaucus. Bioresource Technology 101(15):6059-6068.

54.     Cuihong Du, Xiaoping Yi,Yuanxing Zhang* (2010) Expression and purification of soluble recombinant human endostatin in Escherichia coli. Biotechnology and Bioprocess Engineering 15(2):229-235.

55.     Ping Zhang, Wenwen Zhang, Xiangshan Zhou*, Peng Bai, James M. Cregg,Yuanxing Zhang* (2010) Catabolite repression of Aox in Pichia pastoris is dependent on hexose transporter PpHxt1 and pexophagy. Applied and Environmental Microbiology 76(18): 6108-6118.

56.     XinWang, QiyaoWang, Jingfan Xiao, Qin Liu*, Haizhen Wu,Yuanxing Zhang* (2010) Hemolysin EthA in Edwardsiella tarda is essential for fish invasion in vivo and in vitro and regulated by two-component system EsrA-EsrB and nucleoid protein Hha_Et. Fish and Shellfish Immunology 29(6):1082-1091.

57.     Suxia Li, Haizhen Wu, Jian Zhao, Ling Ou,Yuanxing Zhang* (2010) A biochemistry and molecular biology experiment and evaluation system for biotechnology specialty students. Biochemistry and Molecular Biology Education 38(4): 271-275.

58.     Lili Xu,Qiyao Wang, Jingfan Xiao, Qin Liu*, Xin Wang, Tao Chen,Yuanxing Zhang* (2010) Characterization of Edwardsiella tarda waaL: roles in lipopolysaccharide biosynthesis, stress adaptation, and virulence toward fish. Archives of Microbiology 192(12):1039-1047.

59.    Qiyao Wang, Minjun Yang, Jingfan Xiao, Haizhen Wu, Xin Wang, Yuanzhi Lv, Lili Xu, Huajun Zheng, Shengyue Wang, Guoping Zhao, Qin Liu*,Yuanxing Zhang* (2009) Genome sequence of the versatile fish pathogen Edwardsiella tarda provides insights into its adaptation to broad host ranges and intracellular niches. PLoS One 4(10): e7646.

60.     Haopeng Rui, Qin Liu,Qiyao Wang, Yue Ma, Huan Liu, Cunbin Shi,Yuanxing Zhang* (2009) Role of alkaline serine protease Asp in Vibrio alginolyticus virulence and regulation of its expression by LuxO-LuxR regulatory system. Journal of Microbiology and Biotechnology 19(5): 431-438.

61.     Wei Wei, Haizhen Wu,Hongyan Xu*, Tao Xu, Xiao Zhang, Kangmei Chang,Yuanxing Zhang* (2009) Cloning and molecular characterization of two complement Bf/C2 genes in large yellow croaker (Pseudosciaena crocea). Fish & Shellfish Immunology 27(2):285-295.

62.     XinWang, QiyaoWang*, Jingfan Xiao, Qin Liu, Haizhen Wu, Lili Xu,Yuanxing Zhang* (2009) Edwardsiella tarda T6SS component evpP is regulated by esrB and iron, and plays essential roles in the invasion of fish. Fish and Shellfish Immunology 27(3): 469-477.

63.    Yuanxing Zhang(2009) Approaches to optimizing animal cell culture process: substrate metabolism regulation and protein expression improvement. Advances in Biochemical Engineering/Biotechnology 113: 177-215.

64.    Qiyao Wang, Zhao Yang, Qin Liu*,Yuanxing Zhang* (2009) Surface-exposed expression of Edwardsiella tarda EseB in live attenuated Vibrio anguillarum based on novel surface display systems. Aquaculture Research 40(13):1459-1467.

65.     JingfanXiao, QiyaoWang, Qin Liu, Xin Wang, Huan Liu,Yuanxing Zhang* (2009) Isolation and identification of fish pathogen Edwardsiella tarda from mariculture in China. Aquaculture Research 40(1):13-17.

66.     Menghao Cai, Xiangshan Zhou*, Xueqian Sun, Kejing Tao,Yuanxing Zhang* (2009) Statistical optimization of medium composition for aspergiolide A production by marine-derived fungus Aspergillus glaucus. Journal of Industrial Microbiology & Biotechnology 36(3):381-389.

67.     JingfanXiao, QiyaoWang*, Qin Liu, Lili Xu, Xin Wang, Haizhen Wu,Yuanxing Zhang* (2009) Characterization of Edwardsiella tarda rpoS: its roles in controlling serum resistance, chondroitinase activity, biofilm formation, and autoinducer synthetases expression. Applied Microbiology and Biotechnology 83(1):151-160.

68.     WeiWei, HongyanXu*,Qiyao Wang, Xiao Zhang, Kangmei Chang, Changwen Wu,Yuanxing Zhang* (2009) Identification of differentially expressed genes in large yellow croaker (Pseudosciaena crocea) induced by attenuated live Vibrio anguillarum. Aquaculture 291(1-2):124-129.

69.     Xueqian Sun, Xiangshan Zhou*, Menghao Cai, Kejing Tao,Yuanxing Zhang* (2009) Identified biosynthetic pathway of aspergiolide A and a novel strategy to increase its production in a marine-derived fungus Aspergillus glaucus by feeding of biosynthetic precursors and inhibitors simultaneously. Bioresource Technology 100(18):4244-4251.

70.     Jing Ye, Yue Ma, Qin Liu, Dongling Zhao,Qiyao Wang,Yuanxing Zhang* (2008) Regulation of Vibrio alginolyticus virulence by the LuxS quorum-sensing system. Journal of Fish Diseases 31(3):161-169.

71.     Chun Wei, Xiangshan Zhou,Yuanxing Zhang* (2008) Improving intracellular production of recombinant protein in Pichia pastoris using an optimized preinduction glycerol-feeding scheme. Applied Microbiology and Biotechnology 78(2):257-264.

72.    Zheyong Ma, Xiaoping Yi,Yuanxing Zhang* (2008) Enhanced intracellular accumulation of recombinant HBsAg in CHO cells by dimethyl sulfoxide. Process Biochemistry 43(6):690-695.

73.     Weiyuan Zhang, Xiaoping Yi, Xiangming Sun, Yuanxing Zhang* (2008) Surface modification of non-woven poly (ethylene terephthalate) fabric scaffold for improving cell attachment in animal cell culture. Journal of Chemical Technology and Biotechnology 83(6):904-911.

74.     Yang Tian, Qiyao Wang, Qin Liu, Yue Ma, Xiaodan Cao, Lingyu Guan, Yuanxing Zhang* (2008) Involvement of LuxS in the regulation of motility and flagellar biogenesis in Vibrio alginolyticus. Bioscience, Biotechnology, and Biochemistry 72(4): 1063-1071.

75.     Lingyun Zhou, Qin Liu*, Qiyao Wang, Yue Ma, Yuzhou Xu, Zhao Yang, Yan Zhao, Yuanxing Zhang* (2008) Secretary delivery of heterologous proteins in attenuated Vibrio anguillarum for potential use in vaccine design. Applied Microbiology and Biotechnology 79(6):1027-1034.

76.     Zhao Yang, Qin Liu,Qiyao Wang,Yuanxing Zhang* (2008) Gene cloning and expression of major capsid protein of large yellow croaker iridovirus and its secretion based on a-haemolysin transport system. Journal of the World Aquaculture Society 39(6):774-780.

77.     Zhao Yang, Qin Liu*,Qiyao Wang,Yuanxing Zhang* (2008) Novel bacterial surface display systems based on outer membrane anchoring elements from the marine bacterium Vibrio anguillarum. Applied and Environmental Microbiology 74(14):4359-4365.

78.     Ting Xie, Qin Liu*, Fusheng Xie, Haifeng Liu, Yuanxing Zhang* (2008) Secretory expression of insulin precursor in Pichia pastoris and simple procedure for producing recombinant human insulin. Preparative Biochemistry and Biotechnology 38(3):308-317.

79.     Haopeng Rui, Qin Liu, Yue Ma,Qiyao Wang,Yuanxing Zhang* (2008) Roles of LuxR in extracellular alkaline serine protease A expression, extracellular polysaccharide production and mobility of Vibrio alginolyticus. FEMS Microbiology Letters 285(2):155-162.

80.     Yuzhou Xu, Qin Liu*, Lingyun Zhou, Zhao Yang,Yuanxing Zhang* (2008) Surface display of GFP by Pseudomonas syringae truncated ice nucleation protein in attenuated Vibrio anguillarum strain. Marine Biotechnology 10(6):701-708.

81.     Yang Tian,Qiyao Wang, Qin Liu, Yue Ma, Xiaodan Cao,Yuanxing Zhang* (2008) Role of RpoS in stress survival, synthesis of extracellular autoinducer 2, and virulence in Vibrio alginolyticus. Archives of Microbiology 190(5): 585-594.

82.    Qiyao Wang, Qin Liu, Xiaodan Cao, Minjun Yang,Yuanxing Zhang* (2008) Characterization of two TonB systems in marine fish pathogen Vibrio alginolyticus: their roles in iron utilization and virulence. Archives of Microbiology 190(5): 595-603.

83.     Wenying Wang, Xiaoping Yi,Yuanxing Zhang* (2007) Gene transcription acceleration: main cause of hepatitis B surface antigen production improvement by dimethyl sulfoxide in the culture of Chinese hamster ovary cells. Biotechnology and Bioengineering 97(3):526-535.

84.     Hongzhong Wu, Fan Yang, Shuya Cui, Yufeng Qin, Jianwen Liu*, Yuanxing Zhang* (2007) Hematopoietic effect of fractions from the enzyme-digested colla corii asini on mice with 5-fluorouracil induced anemia. The American Journal of Chinese Medicine 35(5): 853-866.

85.     Lu Yang, Yue Ma, Yuanxing Zhang* (2007) Freeze-drying of live attenuated Vibrio anguillarum mutant for vaccine preparation. Biologicals 35(4):265-269.

86.     Xiangming Sun, Yuanxing Zhang* (2007) A cell-detaching reactor for inoculation of anchorage-dependent CHO and Vero cells between stepwise-expanded bioreactors. Biotechnology Letters 29(5): 697-701.

87.     Qin Liu, Pengbo Wang, Yue Ma, Yuanxing Zhang* (2007) Characterization of Vibrio alginolyticus fur gene and localization of essential amino acid sites in Fur by in vitro site-directed mutagenesis. Journal of Molecular Microbiology and Technology 13(1-3):15-21.

88.     Liming Ouyang, Ling Ou, Yuanxing Zhang* (2007) An integrated teaching strategy for teaching biochemistry to biotechnology specialty students. Biochemistry and Molecular Biology Education 35 (4): 267-271.

89.     Qiyao Wang, Qin Liu, Yue Ma, Haopeng Rui, Yuanxing Zhang* (2007) LuxO controls extracellular protease, hemolytic activities and siderophore production in fish pathogen Vibrio alginolyticus. Journal of Applied Microbiology 103(5):1525-1534.

90.     Qiyao Wang, Qin Liu, Yue Ma, Yuanxing Zhang* (2007) Isolation, sequencing and characterization of cluster genes involved in the biosynthesis and transportation of the siderophore of marine fish pathogen Vibrio alginolyticus. Archives of Microbiology 188(4):433-439.

91.     Haiyan Huang, Yihua Yu, Xiaoping Yi, Yuanxing Zhang* (2007) Nitrogen metabolism of asparagine and glutamate in Vero cells studied by ¹H/¹⁵N NMR spectroscopy. Applied Microbiology and Biotechnology 77(2):427-436.

92.     Fang Zhang, Xiangming Sun, Xiaoping Yi, Yuanxing Zhang* (2006) Metabolic characteristics of recombinant Chinese hamster ovary cells expressing glutamine synthetase in presence and absence of glutamine. Cytotechnology 51(1):21-28.

93.     Anfeng Xiao, Xiangshan Zhou, Li Zhou, Yuanxing Zhang* (2006) Improvement of cell viability and hirudin production by ascorbic acid in Pichia pastoris fermentation. Applied Microbiology and Biotechnology 72(4): 837-844.

94.     Haiyan Huang, Xiaoping Yi, Yuanxing Zhang* (2006) Improvement of Vero cell growth in glutamate-based culture by supplementing ammoniagenic compounds. Process Biochemistry 41(12):2386-2392.

95.     Hechun Zhang, Jixun Zhan, Keman Su, Yuanxing Zhang* (2006) A kind of potential food additive produced by Streptomyces coelicolor: characteristics of blue pigment and identification of a novel compound, l-actinorhodin. Food Chemistry 95(2):186-192.

96.     Junhui Li, Zhengyu Huang, Xiangming Sun, Pengyuan Yang, Yuanxing Zhang* (2006) Understanding the enhanced effect of dimethyl sulfoxide on hepatitis B surface antigen expression in the culture of Chinese hamster ovary cells on the basis of proteome analysis. Enzyme and Microbiol Technology 38(3-4):372-380.

97.     Junhui Li, Xiangming Sun, Yuanxing Zhang* (2006) Improvement of hepatitis B surface antigen expression by dimethyl sulfoxide in the culture of recombinant Chinese hamster ovary cells. Process Biochemistry 41(2):317-322.

98.     Qin Liu, Yue Ma, Lingyun Zhou, Yuanxing Zhang* (2006) Gene cloning, expression and functional characterization of an acyl carrier protein AcpV from Vibrio anguillarum. Archives of Microbiology 185(2):159-163.

99.     Haifeng Liu, Xiangshan Zhou, Yuanxing Zhang* (2006) A comparative investigation on different refolding strategies of recombinant human tissue-type plasminogen activator derivative. Biotechnology Letters 28(7):457-463.

100.  Qin Liu, Yue Ma, Lingyun Zhou, Yuanxing Zhang* (2005) Gene cloning and functional characterization of a phosphopantetheinyl transferase from Vibrio anguillarum serotype O1. Achieves of Microbiology 183(1):37-44.

101.  Subiao Lu, Xiangming Sun, Yuanxing Zhang* (2005) Insight into metabolism of CHO cells at low glucose concentration on the basis of the determination of intracellular metabolites. Process Biochemistry 40(5):1917-1921.

102.  Juxing Chen, Xiangming Sun, Yuanxing Zhang* (2005) Growth and metabolism of marine fish Chinook salmon embryo cells: Response to lack of glucose and glutamine. Biotechnology Letters 27(6): 395-401.

103.  Juxing Chen, Xiangming Sun, Li Zhang, Xiaoping Yi, Yuanxing Zhang* (2005) Mass cultivation of marine fish Chinook salmon embryo cells in bioreactor with low-serum medium. Aquaculture 249(1-4):35-45.

104.  Mingfei Shao, Yue Ma, Qin Liu,Yuanxing Zhang* (2005) Secretory expression of recombinant proteins in an attenuated Vibrio anguillarum strain for potential use in vaccines. Journal of Fish Diseases 28(6):723-728.

105.  Xiangming Sun, Yuanxing Zhang* (2004) Glutamine cannot support recombinant CHO cell growth and maintenance in the absence of glucose. Process Biochemistry 39(6):719-722.

106.  Li Zhang, Hong Shen, Yuanxing Zhang* (2004) Fed-batch culture of hybridoma cells in serum-free medium using an optimized feeding strategy. Journal of Chemical Technology and Biotechnology 79(2):171-181.

107.  Xiaoping Yi, Xiangming Sun, Yuanxing Zhang* (2004) Effects of osmotic pressure on recombinant BHK cell growth and von willebrand factor (vWF) expression. Process Biochemistry 39(11):1817-1823.

108.  Qin Liu, Yue Ma, Haizhen Wu, Mingfei Shao, Haifeng Liu, Yuanxing Zhang* (2004) Cloning, identification and expression of an entE homologue angE from Vibrio anguillarum serotype O1. Archives of Microbiology 181(4):287-293.

109.  Weibin Zhou, Yuanxing Zhang* (2004) Purification and identification of recombinant hirudin and its degradation derivatives expressed in Pichia pastoris. Preparative Biochemistry & Biotechnology 34(3):239-252.

110.  Jizhong Yang, Xiangshan Zhou, Yuanxing Zhang* (2004) Improvement of recombinant hirudin production by controlling NH₄⁺ concentration in Pichia pastoris fermentation. Biotechnology Letter 26(12):1013-1017.

111.  Yuanxing Zhang*, Lin Dai, Xiangming Sun (2003) Kinetic aspect of hepatitis B surface antigen production in recombinant Saccharomyces cerevisiae fermentation. Process Biochemistry 38(11):1593-1598.

112.  Xiangming Sun, Yuanxing Zhang* (2003) Estimation of cell density in packed-bed bioreactor by lactate production rate. Biotechnology Letters 25(11):853-857.

113.  Subiao Lu, Xiangming Sun, Chaoou Shi, Yuanxing Zhang* (2003) Determination of tricarboxylic acid cycle acids and other related substances in cultured mammalian cells by gradient ion-exchange chromatography with suppressed conductivity detection. Journal of Chromatography A 1012(2):161-168.

114.  Xiangshan Zhou, Jian Lu, Weimin Fan, Yuanxing Zhang* (2002) Development of a responsive methanol sensor and its application in Pichia pastoris fermentation. Biotechnology Letters 24(8): 643-646.

115.  Xiangshan Zhou, Yuanxing Zhang* (2002) Decrease of proteolytic degradation of recombinant hirudin produced by Pichia pastoris by controlling the specific growth rate. Biotechnology Letters 24(17):1449-1453.

116.  Jingli Xie, Yuanxing Zhang*, Yonghong Wang, Yuanguang Li (2001) Mixotrophic cultivation of Platymonas subcordiformis. Journal of Applied Phycology 13(4):343-347.

117.  Xiangming Sun, Yuanxing Zhang*, Wensong Tan, Yajing Zhou, Ping Hua (2000) Attachment kinetics of Vero cells onto CT3 microcarriers. Journal of Bioscience and Bioengineering 90(1):32-36.

联系方法：上海市梅陇路130号华东理工大学生物工程学院，200237

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