JU CHU

JU CHU, Professor Ju Chu, from East China University of Science and Technology(ECUST)

      One of the leading bio-processing professors in China who has devoted herself to research on microbial physiology, fermentation process optimization and scale-up for more than 20 years. She has been responsible for some Chinese major scientific projects, the National High Technology Research and Development Program of China (863 Program), the National Scientific and Technological Major Special Project (Significant Creation of New drugs as well as some international collaborative projects, China-Scoland Higher Education Collaboration Project (2010 - 2011) with Prof. Iain Hunter in Strathclyde University, UK. Sino-Dutch Partnership in Research and Innovation(2013-2016), “Rational design and scale-up of aerobic industrial fermentation process” with Prof. Robert Muddle in Delft University of Technology and Prof. Henk Noorman, Royal DSM N.V. in the Netherlands. She has published 4 books and more than 100 papers in international journals.

EDUCATION

BS (ECUST) 1985;  MS (ECUST) 1988;  Ph.D. (ECUST) 1996

ACHIEVEMENTS OF THE PAST FIVE YEARS 

Take charge in the National High Technology Research and Development Program of China (863 Program) “Improvement of the performance of cephalosporin C producing strain Acremonium chrysogenum by metabolic engineering and its fermentation process optimization”, No. 2006AA020302, RMB 6,800,000. (2006-2011).

She has published 136 SCI papers and achieved more than 20 Chinese patents.

WORKING EXPERIENCE

1988-1990,Assistant at East China University of Chemical Technology

1990-1991,Post-Graduate University Course In Microbiology at Faculty of Engineering, Osaka University

1991-1992,Research Fellow at Bath University, UK

1990-1995, Lecturer at East China University of Science & Technology

1995-1999,Associate Professor at East China University of Science & Technology

1999-up to now , Full professor at East China University of Science & Technology

June2007-Dec.2007, Visiting professor of University of Geogia, USA

SELECTED HONORS AND AWARDS

“Industrial fermentation technology based on the integration of microbial physiology and flow field property in bioreactor”, won the Second Prize of  National Award for Science and Technology Progress (2011) by Chinese central government.

“Advanced fermentation technology for improved erythromycin production”, won the first prize of Shanghai Municipal Award for Science and Technology Progress(2008) by the Shanghai municipal government.

“The Key technology for the improvement of “I+G” production”, won the Second Prize of  National Award for Science and Technology Progress(2004)

“Fermentation process optimization and scale up technique based on multi-parameters association”, won the Second Prize of National Award for Science and Technology Progress.(2002)

“Process optimization and scale up of erythromycin fermentation”, awarded second prize of Shanxi Provincial Award for Science and Technology Progress (2002)

“A Multi-scale study on Fermentation process and optimization of a novel additive”, guanosine production, won the first prize of Shanghai Municipal Award for Scientific and Technical Progress(2003)

“Make high specific active gene of phytase and production of phytase resisting high temperature”, awarded the first prize of  Shanghai Municipal Award for Science and Technology Progress (2003)

“Fermentation process optimization and scale up technique based on multi-parameters association and the study on the bioreactor equipments”, awarded the first prize of Shanghai Municipal Award for Science and Technology Progress (2001)

MAJOR RESEARCH INTERESTS

Biochemical/bioprocess engineering; Microbial physiology and its regulation; Fermentation technology for the primary and secondary metabolites; Metabolic engineering

COLLABRATION      

China-Scoland Higher Education Collaboration Project (2010 - 2011) with Prof. Iain Hunter in Strathclyde University, UK.

Sino-Dutch Partnership in Research and Innovation(2013-2016), “Rational design and scale-up of aerobic industrial fermentation process” with Prof. Robert Muddle in Delft University of Technology and Prof. Henk Noorman, Royal DSM N.V. in the Netherlands.

RECENT PUBLICATIONS

1. Guo Qian, Chu Ju*, Zhuang Yingping, Gao Yang. Controlling the feed rate of propanol to optimize erythromycin fermentation by on-line capacitance and oxygen uptake rate measurement [J]. Bioprocess and Biosystems Engineering, 2016, 39(2): 255-265.

2. Tian Xiwei, Wang Yonghong*, Chu Ju*, Zhuang Yingping, Zhang Siliang. Enhanced l-lactic acid production in Lactobacillus paracasei by exogenous proline addition based on comparative metabolite profiling analysis [J]. Applied Microbiology and Biotechnology, 2016, 100(5): 2301-2310.

3. Lu Fei, Wang Zejian, Zhao Wei, Chu Ju*, Zhuang Yingping. A simple novel approach for real-time monitoring of sodium gluconate production by on-line physiological parameters in batch fermentation by Aspergillus niger [J]. Bioresource Technology, 2016, 202: 133-141.

4. Tian Xiwei, Zhang Ning, Yang Yi, Wang Yonghong*, Chu Ju*, Zhuang Yingping, Zhang Siliang. The effect of redox environment on l-lactic acid production by Lactobacillus paracasei—A proof by genetically encoded in vivo NADH biosensor [J]. Process Biochemistry, 2015, 50(12): 2029-2034.

5. Lu Hongzhong, Liu Xiaoyun, Huang Mingzhi*, Xia Jianye, Chu Ju*, Zhuang Yingping, Zhang Siliang, Noorman Henk. Integrated isotope-assisted metabolomics and 13C metabolic flux analysis reveals metabolic flux redistribution for high glucoamylase production by Aspergillus niger [J]. Microbial Cell Factories, 2015, 14(1): 147-160.

6. Lu Fei, Ping Kangkang, Wen Lin, Zhao Wei, Wang Zejian*, Chu Ju*, Zhuang Yingping. Enhancing gluconic acid production by controlling the morphology of Aspergillus niger in submerged fermentation [J]. Process Biochemistry, 2015, 50(9): 1342-1348.

7. Tang Wenjun, Pan Ao, Lu Hongzhong, Xia Jianye*, Zhuang Yingping, Zhang Siliang, Chu Ju*, Noorman Henk. Improvement of glucoamylase production using axial impellers with low power consumption and homogeneous mass transfer [J]. Biochemical Engineering Journal, 2015, 99: 167-176.

8. Lu Hongzhong, Li Chao, Tang Wenjun, Wang Zejian, Xia Jianye*, Zhang Siliang, Zhuang Yingping, Chu Ju*, Noorman Henk. Dependence of fungal characteristics on seed morphology and shear stress in bioreactors [J]. Bioprocess and Biosystems Engineering, 2015, 38(5): 917-928.

9. Shi Fei, Tan Jun, Chu Ju*, Wang Yonghong, Zhuang Yingping, Zhang Siliang. A qualitative and quantitative high-throughput assay for screening of gluconate high-yield strains by Aspergillus niger [J]. Journal of Microbiological Methods, 2015, 109: 134-139.

10. Chen Yong, Wang Zejian, Chu Ju*, Xi Beili, Zhuang Yingping. The glucose RQ-feedback control leading to improved erythromycin production by a recombinant strain Saccharopolyspora erythraea ZL1004 and its scale-up to 372-m3 fermenter [J]. Bioprocess and Biosystems Engineering, 2015, 38(1): 105-112.

11. Wang Guan, Tang Wenjun, Xia Jianye, Chu Ju*, Noorman Henk, van Gulik Walter M.. Integration of microbial kinetics and fluid dynamics toward model-driven scale-up of industrial bioprocesses [J]. Engineering in Life Sciences, 2015, 15(1): 20-29.

12. Tan Jun, Chu Ju*, Wang Yonghong, Zhuang Yingping, Zhang Siliang. High-throughput system for screening of Monascus purpureus high-yield strain in pigment production [J]. Bioresources and Bioprocessing, 2014, 1(1): 1-7.

13. Zhang Qiang, Chen Yong, Hong Ming, Gao Yang, Chu Ju*, Zhuang Yingping, Zhang Siliang. The dynamic regulation of nitrogen and phosphorus in the early phase of fermentation improves the erythromycin production by recombinant Saccharopolyspora erythraea strain [J]. Bioresources and Bioprocessing, 2014, 1(1): 1-6.

14. Tian Xiwei, Wang Yonghong*, Chu Ju*, Zhuang Yingping, Zhang Siliang. Oxygen transfer efficiency and environmental osmolarity response to neutralizing agents on l-lactic acid production efficiency by Lactobacillus paracasei [J]. Process Biochemistry, 2014, 49(12): 2049-2054.

15. Tian Xiwei, Wang Yonghong*, Chu Ju*, Zhuang Yingping, Zhang Siliang. l-Lactic acid production benefits from reduction of environmental osmotic stress through neutralizing agent combination [J]. Bioprocess and Biosystems Engineering, 2014, 37(9): 1917-1923.

16. Wang Guan, Chu Ju*, Noorman Henk, Xia Jianye, Tang Wenjun, Zhuang Yingping, Zhang Siliang. Prelude to rational scale-up of penicillin production: a scale-down study [J]. Applied Microbiology and Biotechnology, 2014, 98(6): 2359-2369.

17. Zhu Pei, Chen Daijie, Liu Wenbin, Zhang Jianbin, Shao Lei, Li Ji-an, Chu Ju*. Hydroxylation and hydrolysis: two main metabolic ways of spiramycin I in anaerobic digestion [J]. Bioresource Technology, 2014, 153: 95-100.

18. Chen Yong, Huang Mingzhi, Wang Zejian, Chu Ju*, Zhuang Yingping, Zhang Siliang. Controlling the feed rate of glucose and propanol for the enhancement of erythromycin production and exploration of propanol metabolism fate by quantitative metabolic flux analysis [J]. Bioprocess and Biosystems Engineering, 2013, 36(10): 1445-1453.

19. Tan Jun, Chu Ju*, Hao Yuyou, Wang Yonghong, Yao Sichao, Zhuang Yingping, Zhang Siliang. A high-throughput screening strategy for accurate quantification of erythromycin [J]. Journal of the Taiwan Institute of Chemical Engineers, 2013, 44(4): 538-544.

20. Chen Yong, Wang Zejian, Chu Ju*, Zhuang Yingping, Zhang Siliang, Yu Xiaoguang. Significant decrease of broth viscosity and glucose consumption in erythromycin fermentation by dynamic regulation of ammonium sulfate and phosphate [J]. Bioresource Technology, 2013, 134: 173-179.

21. Tan Jun, Chu Ju*, Hao Yuyou, Guo Yuanxin, Zhuang Yingping, Zhang Siliang. High-throughput system for screening of Cephalosporin C high-yield strain by 48-deep-well microtiter plates [J]. Applied Biochemistry and Biotechnology, 2013, 169(5): 1683-1695.

22. Chu Ju*, Qian Jiangchao, Zhuang Yingping, Zhang Siliang, Li Yourong. Progress in the research of S-adenosyl-L-methionine production [J]. Applied Microbiology and Biotechnology, 2013, 97(1): 41-49.

23. Pethick Florence E., MacFadyen Alison C., Tang Zhenyu, Sangal Vartul, Liu Tze-Tze, Chu Ju, Kosec Grefor, Petkovic Hrvoje, Guo Meijn, Kirby Ralph, Hoskisson Paul A., Herron Paul R., Hunter Iain S*.. Draft genome sequence of the oxytetracycline-producing bacterium Streptomyces rimosus ATCC 10970 [J]. Genome Announc, 2013, 1(2): e00063-13.

24. Tan Jun, Chu Ju*, Shi Wenjuan, Lin Cheng, Guo Yuanxin, Zhuang Yingping, Zhang Siliang, Imanaka Tadayuki. High-throughput screening strategy used for enhanced production of pigment by Monascus purpureus D39-4 [J]. Food Science and Biotechnology, 2012, 21(6): 1603-1610.

25. Yang Yiming, Xia Jianye*, Li Jianhua, Chu Ju*, Li Liang, Wang Yonghong, Zhuang Yingping, Zhang Siliang. A novel impeller configuration to improve fungal physiology performance and energy conservation for cephalosporin C production [J]. Journal of Biotechnology, 2012, 161(3): 250-256.

26. Zou Xiang, Xia Jianye, Chu Ju*, Zhuang Yingping, Zhang Siliang. Real-time fluid dynamics investigation and physiological response for erythromycin fermentation scale-up from 50 L to 132 m3 fermenter [J]. Bioprocess and Biosystems Engineering, 2012, 35(5): 789-800.

27. Wu Dan, Chu Ju*, Hao Yuyou, Wang Yonghong, Zhuang Yingping, Zhang Siliang. Incomplete protein disulphide bond conformation and decreased protein expression result from high cell growth during heterologous protein expression in Pichia pastoris [J]. Journal of Biotechnology, 2012, 157(1): 107-112.

28. Liang Jianguang, Chu Xiaohe, Xiong Zhiqiang, Chu Ju*, Wang Yonghong*, Zhuang Yingping, Zhang Siliang. Oxygen uptake rate regulation during cell growth phase for improving avermectin B1a batch fermentation on a pilot scale (2 m3) [J]. World Journal of Microbiology and Biotechnology, 2011, 27(11): 2639-2644.

29. Wu Dan, Chu Ju*, Hao Yuyou, Wang Yonghong, Zhuang Yingping, Zhang Siliang. High efficient production of recombinant human consensus interferon mutant in high cell density culture of Pichia pastoris using two phases methanol control [J]. Process Biochemistry, 2011, 46(8): 1663-1669.

30. Qin Xiulin， Qian Jiangchao*， Yao Gaofeng， Zhuang Yingping， Zhang Siliang，Chu Ju*， GAP Promoter Library for Fine-Tuning of Gene Expression in Pichia pastoris，A PPLIED AND ENVIRONMENTAL MICROBIOLOGY ，2011，77 （11）: 3600-3608

31.Qin X.，Qian J*.，Xiao C.， Zhuang Y.，Zhang S.， Chu J.*， Reliable high-throughput approach for screening of engineered constitutive promoters in the yeast Pichia pastoris LETTERS IN APPLIED MICROBIOLOGY，2011， 52（6 ）: 634-641

32. Zou Xiang，Li Wan-jun，Zeng Wei，Chu Ju*，Zhuang Ying-ping，Zhang Si-liang， An assessment of seed quality on erythromycin production by recombinant Saccharopolyspora erythraea strain ，BIORESOURCE TECHNOLOGY，2011，102 （3）: 3360-3365

33.Li Jianhua，Yang Yiming，Chu Ju*，Huang, Mingzhi*，Li Liang，Zhang Xiaochun，Wang Yonghong，Zhuang Yingping，Zhang Siliang， Quantitative metabolic flux analysis revealed uneconomical utilization of ATP and NADPH in Acremonium chrysogenum fed with soybean oil， BIOPROCESS AND BIOSYSTEMS ENGINEERING ，2010 ，33  （ 9）  : 1119-1129  

34.Zou Xiang，Zeng Wei，Chen Chang-fa，Qi Xia-chang，Qian Jiang-chao，Chu Ju*，Zhuang, Ying-ping，Zhang Si-liang，Li Wan-jun，Fermentation Optimization and Industrialization of Recombinant Saccharopolyspora erythraea Strains for Improved Erythromycin A Production ，BIOTECHNOLOGY AND BIOPROCESS ENGINEERING  ，2010 ， 15 （6 ）: 959-968

35.Liang Jian-Guang, Chu Xiao-He, Chu Ju* , Wang Yong-Hong, Zhuang Ying-Ping, Zhang, Si-Liang  Oxygen uptake rate (OUR) control strategy for improving avermectin B-1a production during fed-batch fermentation on industrial scale (150 m(3)) ,AFRICAN JOURNAL OF BIOTECHNOLOGY ,2010 ,9(42) :7186-7191  

36.Zou X, Chen C. –F, Huang H.-F, Chu J*, Zhuang Y-P, Zhang S-L, Response Surface Methodology for Optimization of the Erythromycin Production by Fed-Batch Fermentation Using an Inexpensive Biological Nitrogen Source, CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY, 2010,24(1): 95-2010

37.Xiang Zou , Hai-feng Hang , Ju Chu*, Ying-ping Zhuang, Si-liang Zhang, Oxygen uptake rate optimization with nitrogen regulation for erythromycin production and scale-up from 50 L to 372 m3 scale. Bioresource Technology. 2009, 100 :1406–1412

38.Xiang Zou, Hai-feng Hang , Ju Chu*, Ying-ping Zhuang, Si-liang Zhang , Enhancement of erythromycin A production with feeding available nitrogen sources in erythromycin biosynthesis phase，Bioresource Technology,2009,100 (13) :3358-3365

39.Wang Y, Chu J, Zhuang YP, Wang YH, Xia JY, Zhang SL*, Industrial bioprocess control and optimization in the context of systems biotechnology, Biotechnology Advances,2009, 27(6): 989-995  

40. Jin Zhou , Yong-Hong Wang*, Ju Chu*, Ling-Zhi Luo, Ying-Ping Zhuang, Si-Liang Zhang,Optimization of cellulase mixture for efficient hydrolysis of steam-exploded corn stover by statistically designed experiments. BioresourceTechnology ,2009,100 :819–825

41. 18. Zhen-Lin Li, Yong-Hong Wang* , Ju Chu* , Ying-Ping Zhuang and Si-Liang Zhang，Leucine improves the component of isovalerylspiramycins for the production of bitespiramycin，Bioprocess & Biosystem Engineering, 2009, 32: 641-647

42. Hui Hu, Jiangchao Qian*, Ju Chu*, Yonghong, Yingping Zhuang , Siliang Zhang,

Optimization of l-methionine feeding strategy for improving S-adenosyl-l-methionine production by methionine adenosyltransferase overexpressed Pichia pastoris，Applied Microbiology & Biotechnology, 2009, 83 (6): 1105-1114

43. Hui Hu, Jiangchao Qian*, Ju Chu*, Yonghong, Yingping Zhuang , Siliang Zhang,DNA shuffling of methionine adenosyltransferase gene leads to improved S-adenosyl-l-methionine production in Pichia pastoris, Journal of Biotechnology, 2009, 141: 97–103

44.Xiang Zou, Hai-feng Hang, Chang-fa Chen, Ju Chu*, Ying-ping Zhuang, Si-liang Zhang，Application of oxygen uptake rate and response surface methodology for erythromycin production by Saccharopolyspora erythraea. J Ind Microbiol Biotechnol 2008, 35:1637–1642

45.Yun Chen, Wei Deng, Jiequn Wu, Jiangchao Qian, Ju Chu, Yingping Zhuang, Siliang Zhang and Wen Liu*. Genetic Modulation of the Overexpression of Tailoring Genes eryK and eryG Leading to the Improvement of Erythromycin A Purity and Production in Fermentation of Saccharopolyspora erythraea. Applied and Environmental Microbiology. 2008, 74(6): 1820-1828.

46. Zhou J, Wang YH*, Chu J*, Zhuang YP, Zhang SL and Yin P. Identification and purification of the main components of cellulases from a mutant strain of Trichoderma viride T 100-14. Bioresource Technology, 2008, 99: 6826–6833.

CONTACT DETAILS

130 Meilong Road, Shanghai 200237, China

Tel: +86-21-64253021; Fax: +86-21-64253702; E-mail: juchu@eucst.edu.cn