Feng-Qing Wang

Feng-Qing Wang, Ph.D.

State Key Laboratory of Bioreactor Engineering

East China University of Science and Technology (ECUST)

Rm. 401, 14th Lab Building, 130 Mei Long Rd.

Shanghai, 200237, P.R.China

Phone: +86-21-64253287, Fax: +86-21-64250068

E-mail: fqwang@ecust.edu.cn

EDUCATION

2003-2007   Ph.D. in Biochemical Engineering, East China University of Science and Technology (ECUST)

2000-2003   M.S. in Biochemical Engineering, Tianjin University of Science and Technology (TUST)

1996-2000   B.S. in Biochemical Engineering, TUST

RESEARCH EXPERIENCE

2012-present  Associate Professor of National Key Laboratory of Bioreactor Engineering, ECUST

2007-2012   Assistant Professor of National Key Laboratory of Bioreactor Engineering, ECUST

RESEARCH INTERESTS

     Our research has focused on the development and improvement of “microbial cell factories” to produce chemicals, natural products, and so on. The main works are carried out on the basis of actinomycetes and yeasts using the principles and methods of metabolic engineering and synthetic biology. There are two topics. Topic 1 is to modify microbial strains applied in the industry to overcome their deficiencies in applications by the researches on their physiological and biochemical properties, omics data and metabolic engineering. Topic 2 is to develop new microbial strains to achieve the innovative synthesis of chemicals, natural products from animals and plants, and drugs by the combination of the methods of metabolic engineering and synthetic biology. Currently, our main projects include the development of microbial cell factories for the production of steroids, active ingredients of Chinese herbal medicine, natural spices and microbial secondary metabolites. Our ultimate goal is to achieve the production of these high value compounds inexpensively, efficiently and environmental friendly.

PROJECTS      

2014-2017     National Natural Science Foundation of China No. 31370080

2013-2015     National Natural Science Foundation of China No. 21206039

2012-2014     National Special Funds for State Key Laboratory of Bioreactor

2011-2013     Fundamental Research Funds for the Central Universities of China

2008-2010     National High Technology Research and Development Program ("863" Program) of China NO. 2008AA02Z209

2008-2010     National Natural Science Foundation of China No. 20777016

SELECTED PUBISHED PAPERS (* corresponding author)

1. Li-Qin Xu, Yong-Jun Liu, Kang Yao, Hao-Hao Liu, Xin-Yi Tao, Feng-Qing Wang*, Dong-Zhi Wei. Unraveling and engineering the production of 23,24-bisnorcholenic steroids in sterol metabolism. Scientific Reports, 2016, 6:21928.

2. Xin-Bin Liu, Min Liu, Xin-Yi Tao, Zhong-Xi Zhang, Feng-Qing Wang*, Dong-Zhi Wei. Metabolic engineering of Pichia pastoris for the production of dammarenediol-II. Journal of biotechnology, 2015, 216: 47-55.

3. Kang Yao, Li-Qin Xu, Feng-Qing Wang*, Dong-Zhi Wei. Characterization and engineering of 3-ketosteroid-Δ1-dehydrogenase and 3-ketosteroid-9α-hydroxylase in Mycobacterium neoaurum ATCC 25795 to produce 9α-hydroxy-4-androstene-3,17-dione through the catabolism of sterols. Metabolic Engineering, 2014, 24: 181-191.

4. Hengwei Wang, Fengqing Wang, Wei Wang, Xueling Yao, Dongzhi Wei, Hairong Cheng, Zixin Deng. Improving the expression of recombinant proteins in E. coli BL21 (DE3) under acetate stress: an alkaline pH shift approach. Plos One. 2014, 9(11): e112777.

5. Kang Yao, Feng-Qing Wang*, Huai-Cheng Zhang, Dong-Zhi Wei. Identification and engineering of cholesterol oxidases involved in the initial step of sterols catabolism in Mycobacterium neoaurum. Metabolic Engineering, 2013, 15:75-87.

6. Wei Wei, Shu-Yue Fan, Feng-Qing Wang*, Dong-Zhi Wei. Accumulation of androstadienedione by overexpression of heterologous 3-ketosteroid-C1-dehydrogenase in Mycobacterium neoaurum NwIB-01. World Journal of Microbiology and Biotechnology. 2014, 30: 1947-1954.

7. Miao-Miao Chen, Feng-Qing Wang*, Liang-Cai Lin, Kang Yao, Dong-Zhi Wei. Characterization and application of fusidane antibiotic biosynethsis enzyme 3-ketosteroid-?1-dehydrogenase in steroid transformation. Applied Microbiology and Biotechnology, 2012, 96:133-142.

8. Hengwei Wang, Fengqing Wang, Xinyi Tao Hairong Cheng. Ammonia-containing DMSO: An improved solvent for the dissolution of formazan crystals in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Analytical Biochemistry, 2012, 421:324-326.

9. Liangcai Lin, Weiguo Fang, Xinggang Liao, Fengqing Wang, Dongzhi Wei, Raymond J. St. Leger. The MrCYP52 cytochrome P450 monoxygenase gene of Metarhizium robertsii is important for utilizing insect epicuticular hydrocarbons. PloS One. 2011, 6(12) e28984.

10. Liangcai Lin, Fengqing Wang*, Dongzhi Wei. Chlorimuron ethyl as a new selectable marker for disrupting genes in the insect-pathogenic fungus Metarhizium robertsii. Journal of Microbiological Methods, 2011, 87:241-243.

11. Feng-Qing Wang, Kang Yao, Dongzhi Wei. From soybean phytosterols to steroid hormones. In Hany A. EI-Shemy (ed.) Soybean and Health. Intech, 2011, P231-252.

12. Hengwei Wang, Hairong Cheng, Dongzhi Wei, Fengqing Wang*. Comparison of methods for measuring viable E. coli cells during cultivation: Great differences in the early and late exponential growth phases. Journal of Microbiological Methods, 2011, 84:140-143.

13. Wei Wei, Fengqing Wang*, Shu-yue Fan, Dong-zhi Wei. Inactivation and augmentation of the main 3-ketosteroid-Mycobacterium neoaurum NwIB-01: biotransformation of soybean phytosterols to 4-androstene-3,17-dione or 1,4-androstadiene-3,17-dione. Applied and Environmental Microbiology, 2010, 76:4578-4582.

14. Wei Wei, Shuyue Fan, Fengqing Wang*, Dongzhi Wei. A new steroid-transforming strain of Mycobacterium neoaurum and cloning of 3-ketosteroid 9α-hydroxylase in NwIB-01. Applied Biochemistry and Biotechnology. 2010, 162:1446-1456.

15. Bo Li, Wei Wang, Feng-Qing Wang*，Dong-Zhi Wei. Cholesterol oxidase ChoL is a critical enzyme that catalyzes the conversion of diosgnin to 4-ene-3-keto steroids in Streptomyces virginiae IBL-14. Applied Microbiology and Biotechnology, 2010, 85:1831-1838.

16. HengWei Wang, Fengqing Wang, Dongzhi Wei. Impact of oxygen supply on rtPA expression in Escherichia coli BL21 (DE3): ammonia effects. Applied Microbiology and Biotechnology, 2009, 82:249-259.

17. Feng-Qing Wang, Cheng-Gang Zhang, Bo Li, Dong-Zhi Wei, Wang-Yu Tong. New microbiological transformations of steroids by Streptomyces virginiae IBL-14. Environmental Science & Technology. 2009, 43:5967-5974.

18. Wei Wang, Feng-Qing Wang, Dong-Zhi Wei. Characterization of P450 FcpC, the enzyme responsible for bioconversion of diosgnone to isonuatigenone in Streptomyces virginiae IBL-14. Applied and Environmental Microbiology. 2009, 75:4202-4205.

19. Feng-Qing Wang, Bo Li, Wei Wang, Cheng-Gang Zhang, Dong-Zhi Wei.  Biotransformation of diosgenin to nuatigenin-type steroid by a newly isolated strain, Streptomyces virginiae IBL-14. Applied Microbiology and Biotechnology. 2007, 77:771-777.