Yi Yang

Author： CreatedTime：2018-09-05 21:09:00

Yi Yang, Ph. D.

Laboratory URL: http: //webmanage.ecust.edu.cn/s/48/t/239/a/9479/info.jspy

E-mail: yiyang@ecust.edu.cn

Tel: 021-64251311

Dr. Yang holds a B. S. degree in Biological Science and Biotechnology and Ph. D. degree in Biochemistry from Tsinghua University. From 1999 to 2005, he worked as research fellow in B.L. Vallee’s lab in Harvard Medical School, and then in J.L Loscalzo’s lab in Boston University School of Medicine. In 2005, he returned to Harvard Medical School as Instructor of Medicine. Dr. Yang joined the faculty of newly founded School of Pharmacy, and State Key Laboratory of Bioreactor in East China University of Science and Technology in 2006, as a Specially Appointed Professor of Biochemistry. In 2014, he became the deputy director of the State Key Laboratory of Bioreactor Engineering.

Dr. Yang has authored or co-authored more than 40 research articles, many of them published in high profile journals such as Nature Methods, Cell Metabolism, J Am Chem Soc, Angew. Chem., PNAS and Embo J. He also filed more than 10 patents, with three of them already issued. The optogenetic technologies developed in Dr. Yang’s lab are currently utilized in more than 230 labs worldwide. Over the past ten years, Dr. Yang received many awards including National Outstanding Young Scientist Award in 2012 , MOST young scientific and technological innovation leading talent in 2014, Chang Jiang Scholars in 2015, Chinese Overseas Contribution Award (innovation) in 2014 , Shanghai Subject Chief Scientist Award in 2014, Dawn Scholar Award in 2011, Eastern Scholar Award in 2008.

Description of Research

1. Genetically encoded sensors for cellular metabolites and life activities. We are currently developing protein based sensors by fusion of fluorescent proteins and specific sensing domains. These sensors may be used conveniently for monitoring various intracellular events. Particularly, we have recently obtained highly responsive NADH, glucose and cAMP sensors for live cell imaging.

2. Small molecular fluorescent probes for specific labeling and imaging of protein thiol post translational modifications, including protein S-nitrosation, disulfides, sulfenic acid and vicinal dithiols. We developed new fluorescent imaging methods for cellular protein disulphide, S-nitrosation, sulfenic acid and vicinal dithiols. Ongoing studies include regulation of protein thiol proteome and their functional implications.

3. Optogenetics modules and circuits. We are currently developing synthetic proteins which are activated by light. These proteins may be used for control of gene expression, enzyme catalysis and labeling of live cells and animals in a spatiotemporal manner.

4. High content, high throughput genetic and drug screening. Our new methodologies make it possible to identify genes and chemicals that regulate global cellular metabolism in a large scale. These genes and chemicals have the potential to be used in disease diagnosis and reatments.

5. Protein expression systems and bio-manufacturing technologies. We are currently working on next generation bioreactor technologies with improved efficiency and reduced emission.

Selected Publications

1. Zhao, Y., Hu, Q., Cheng, F., Su, N., Wang, A., Zou, Y., Hu, H., Chen, X., Zhou, H.M., Huang, X., Yang, K., Zhu, Q., Wang, X., Yi, J., Zhu, L., Qian, X., Chen, L., Tang, Y., Loscalzo, J., and Yang, Y*. (2015). SoNar, a Highly Responsive NAD(+)/NADH Sensor, Allows High-Throughput Metabolic Screening of Anti-tumor Agents. Cell Metab 21: 777-789.

2. Chen, X., Li, T., Wang, X., Du, Z., Liu, R., and Yang, Y. * (2015). Synthetic dual-input mammalian genetic circuits enable tunable and stringent transcription control by chemical and light. Nucleic Acids Research 10.1093/nar/gkv1343.

3. Oldham, W.M., Clish, C.B., Yang, Y., and Loscalzo, J. (2015). Hypoxia-Mediated Increases in l-2-hydroxyglutarate Coordinate the Metabolic Response to Reductive Stress. Cell Metab 22: 291-303.

4. Yang, H., Zhou, L., Shi, Q., Zhao, Y., Lin, H., Zhang, M., Zhao, S., Yang, Y., Ling, Z.Q., Guan, K.L., Xiong, Y., and Ye, D. (2015). SIRT3-dependent GOT2 acetylation status affects the malate-aspartate NADH shuttle activity and pancreatic tumor growth. EMBO J 34: 1110-1125.

5. Zhao, Y., and Yang, Y. * (2015). Profiling metabolic states with genetically encoded fluorescent biosensors for NADH. Curr Opin Biotechnol 31: 86-92.

6. Huang, C., Jia, T., Tang, M., Yin, Q., Zhu, W., Zhang, C., Yang, Y., Jia, N., Xu, Y., and Qian, X. (2014). Selective and Ratiometric Fluorescent Trapping and Quantification of Protein Vicinal Dithiols and in Situ Dynamic Tracing in Living Cells. J Am Chem Soc 136: 14237-14244

7. Wang, Y.P., Zhou, L.S., Zhao, Y.Z., Wang, S.W., Chen, L.L., Liu, L.X., Ling, Z.Q., Hu, F.J., Sun, Y.P., Zhang, J.Y., Yang, C., Yang, Y., Xiong, Y., Guan, K.L., and Ye, D. (2014). Regulation of G6PD acetylation by SIRT2 and KAT9 modulates NADPH homeostasis and cell survival during oxidative stress. EMBO J 33: 1304-1320.

8. Wang, X., Chen, X., and Yang, Y. * (2012). Spatiotemporal control of gene expression by a light-switchable transgene system. Nat Methods 9: 266-269.

9. Huang, C., Yin, Q., Zhu, W. *, Yang, Y. *, Wang, X., Qian, X., and Xu, Y. * (2011). Highly Selective Fluorescent Probe for Vicinal-Dithiol-Containing Proteins and In Situ Imaging in Living Cells. Angew Chem Int Ed Engl 123: 7693-7698.

10. Zhao, Y., Jin, J., Hu, Q., Zhou, H.M., Yi, J., Yu, Z., Xu, L., Wang, X., Yang, Y. *, and Loscalzo, J. (2011). Genetically Encoded Fluorescent Sensors for Intracellular NADH Detection. Cell Metab 14: 555-566.

11. Yang, Y., Song, Y., and Loscalzo, J. (2007). Regulation of the protein disulfide proteome by mitochondria in mammalian cells. Proc Natl Acad Sci U S A 104: 10813-10817.

12. Yang, Y., and Loscalzo, J. (2005). S-nitrosoprotein formation and localization in endothelial cells. Proc Natl Acad Sci U S A 102: 117-122.

13. Yang, Y., Maret, W., and Vallee, B.L. (2001). Differential fluorescence labeling of cysteinyl clusters uncovers high tissue levels of thionein. Proc Natl Acad Sci U S A 98: 5556-5559.

Media coverage:

Nature Methods Author Profile： http://www.nature.com/nmeth/journal/v9/n3/full/nmeth.1901.html

Sciencenet：

http://news.sciencenet.cn/htmlnews/200773182721241185764.html

http://news.sciencenet.cn/htmlpaper/2011103110464332220238.shtm

http://news.sciencenet.cn/htmlpaper/201221411152022022318.shtm

http://news.sciencenet.cn/htmlnews/2015/5/318392.shtm

Ebiotrade：

http://www.ebiotrade.com/newsf/2012-3/201231593621534.htm

http://www.ebiotrade.com/newsf/2012-3/2012315110514627.htm

http://www.ebiotrade.com/custom/ebiotrade/zt/130923/index.htm

http://www.ebiotrade.com/newsf/2013-3/201331165546667.htm

http://www.ebiotrade.com/newsf/2015-5/201557111541729.htm

ECUST：

http://news.ecust.edu.cn/news/34545

http://news.ecust.edu.cn/news/34366

http://news.ecust.edu.cn/news/30129

http://news.ecust.edu.cn/news/23084