Huilei Yu

Dr. HUILEI YU

2003.09 – 2008.04 Ph.D. in Biochemical Engineering (Prof. Jian-He Xu Group)

                 East China University of Science and Technology (ECUST)

2005.09 – 2007.04 Visiting Researcher in Prof. Guo-Qiang Lin Group, Shanghai

                 Institute of Organic Chemistry, Chinese Academic of Sciences

2008.04 – 2010.09 Assistant Professor, Lab of Biocatalysis and Bioprocessing,

                 State Key Laboratory of Bioreactor Engineering, ECUST

2010.09 – Present Associate Professor, Lab of Biocatalysis and Bioprocessing,

                 State Key Laboratory of Bioreactor Engineering, ECUST

2012.08                                    Visiting Researcher in Prof. T Imanaka Group

                 Ritsumeikan University, Japan

2013.06 – 2014.06 Visiting Professor in Bioinformatics & Metabolic Engineering Laboratory (Prof. Gregory Stephanopoulos’ Group)

Massachusetts Institute of Technology, USA

Research Interests

l  Genomic mining and evolution of oxidoreductase

l  Multi-enzymatic reaction for combinatorial biocatalysis

l  Biocatalytic Synthesis and/or Resolution of Chiral Compounds

Recent paper

1.       Yin YC, Yu HL*, Luan ZJ, Li RJ, Ouyang PF, Liu J, Xu JH. Unusually broad substrate profile of a self-sufficient cytochrome P450 monooxygenase CYP116B4 mined from Labrenzia aggregate. ChemBioChem 2014, 25(16): 2443-2449.

2.       Huang L, Ma HM, Yu HL* and Xu JH*. Altering the substrate specificity of a reductase CgKR1 from Candida glabrata by protein engineering for bioreduction of aromatic α-keto esters. Adv.Synth.Catal. 2014, 356(9): 1943-1948.

3.       Xu YP, Guan Y H, Yu HL*, Ni Y, Ma BD, Xu JH*. Improved o-chlorobenzoylformate bioreduction by stabilizing aldo-keto reductase YtbE with additives. J Mol Catal B: Enzymatic 2014, 104: 108-114.

4.       Ma BD, Kong XD, Yu HL*, Zhang ZJ, Dou S, Xu YP, Ni Y, and Xu JH *. Increased Catalyst Productivity in α-Hydroxy Acids Resolution by Esterase Mutation and Substrate Modification. ACS Catal. 2014, 4 (3): 1026–1031

5.       Zhang WX, Xu GC, Huang L, Pan J, Yu HL*, Xu JH*. Highly efficient synthesis of (R)-3-quinuclidinol in a space–time yield of 916 g L^–1 d^–1 using a new bacterial reductase ArQR. Org. Lett. 2013, 15(19): 4917–4919

6.       Xu GC, Yu HL*, Zhang ZJ, Xu JH*. Stereocomplementary bioreduction of β-ketonitrile without ethylated byproduct. Org. Lett. 2013, 15(21): 5408–5411

7.       Ma BD, Yu HL*, Pan J, Liu JY, Ju X, Xu JH*.A thermostable and organic-solvent tolerant esterase fromPseudomonas putida ECU1011: Catalytic properties and performance in kinetic resolution of alpha-hydroxy acids.Bioresour. Technol. 2013, 133: 354-360

8.       Xu GC, Yu HL*, Zhang XY and Xu JH*. Access to optically active aryl halohydrins using a substrate-tolerant carbonyl reductase discovered from Kluyveromyces thermotolerans. ACS Catal. 2012, 2(12): 2566–2571

9.       Zou ZZ, Yu HL*, Li CX, Zhou XW, Hayashi C, Sun J, Liu BH, Imanaka T, Xu JH*. A new thermostable beta-glucosidase mined from Dictyoglomus thermophilum: Properties and performance in octyl glucoside synthesis at high temperatures. Bioresour. Technol. 2012, 118: 425-430

10.   Yu HL, Ou L, Xu JH*. New trends in non-aqueous biocatalysis. Curr. Org. Chem. 2010, 14(14): 1424-1432

11.   Yu HL, Xu JH*, Lu WY, Lin GQ. Discovery and utilization of biocatalysts for chiral synthesis: An overview of Chinese scientists research and development. Adv. Biochem. Eng. Biotechnol. 2009, 113: 1-31

12.   Yu HL, Xu JH*, Lu WY, Lin GQ. Environmentally benign synthesis of natural glycosides using apple seed meal as green and robust biocatalyst. J. Biotechnol. 2008, 133: 469-477

13.   Yu HL, Xu JH*, Wang YX, Lu WY, Lin GQ. Assembly of a three-dimensional array of glycoconjugates by combinatorial biocatalysis in nonaqueous media. J. Comb. Chem. 2008, 10: 79-87

Presentations

[1] Efficient synthesis of alkyl glucosides by an extremely thermostable recombinant beta-glucosidase from Dictyoglomus thermophilum. 14th Asia Pacific Confederation of Chemical Engineering Congress. 2012, Singapore. Oral presentation.

[2] Production of l-menthol by a newly isolated Bacillus subtilis ECU0554 at a high substrate concentration. 238th ACS National Meeting & Exposition. 2009, Washington D.C. USA. Poster.

[3] Construction of glycoconjugates array by combinatorial biocatalysis. 12th Asia Pacific Confederation of Chemical Engineering Congress. 2008, Dalian, China. Oral presentation.

[4] Assembly a three-dimensional array of glycoconjugates by combinatorial biocatalysis in nonaqueous media. ISCET-ISBBE. 2007, Shanghai, China. Oral presentation.

[5] Construction of a natural glycoside esters library by combinatorial biocatalysis of glycosidase and lipase. The 13th symposium of young asian biochemical engineers’ community. 2007, Seoul, Korea. Poster.

Room 911, Research Building No.18, 130 Meilong Road, Shanghai 200237 P.R.C.

T: +86-021-64250840, Fax: +86-21-6425-0840; E-mail: huileiyu@ecust.edu.cn