Yuanxing Zhang

Introduction to Professor Yuanxing Zhang

    Professor Yuanxing Zhang is a professor in East China University of Science and Technology since 1995 and presently Director of the Shanghai Engineering Research Center of Maricultured Animal Vaccines. He was appointed the member and vice director of the Expert Group for Marine Biotechnology Division of National 863 High-Tech Program of China (1999-2010). He has published more than 390 papers and 2 monographs, won 6 Science and Technology Advancement Prices of provincial and ministerial levels, applied 53 national invention patents and 3 PCT/US patents.

Research Interests in His Team with 10 Members
1. Marine biotechnology. The characteristics and pathogenicity of marine pathogenetic bacterium are investigated and attenuated vaccine is developed by the genetic modification of the bacterium. The quorum sensing and its regulation genes are studied in pathogen bacteria. A surface display system on bacterium cell is established to express some antigen protein for potential multivalent vaccine use. Currently, based on the genome informatics and important functional genes of a pathogen bacterium, Edwardsiella tarda, attenuated vaccines for maricultured fish are being evaluated. 
2. Microbiological engineering. In methanol yeast, the precision regulatory networks of gene induction are studied, and a new Pichia pastoris expression system with different induction mechanism is being constructed. With gene expression regulated, recombinant microorganisms are cultivated in high densities for the efficient production of recombinant proteins. Currently, metabolic regulation and morphological shape of marine-derived filamentous fungi are studied to prepare active compounds, and some extremophiles from deep sea or the South Pole are cultured in large scale.

Books (Chinese):
1. Yuanxing Zhang, Xiaoping Yi, Li Zhang, Xiangming Sun (2007) Animal Cell Culture Engineering. Beijing: Chemical Industry Press.
2. Yuanxing Zhang, Xueshu Xu (2001) Bioreactor Engineering. Shanghai: East China University of Science and Technology Press.

Some papers published in recent five years:
1 Tao Jiang, Mengmeng Huang, Hao He, Jian Lu, Xiangshan Zhou, Menghao Cai*, Yuanxing Zhang. Bioprocess exploration for thermostable α-amylase production of a deep-sea thermophile Geobacillus sp. in high temperature bioreactor. Preparative Biochemistry & Biotechnology 2015, accepted.
2 Ni Ye, Haizhen Wu*, Qiyao Wang, Qin Liu, Jingfan Xiao, Yuanxing Zhang. Maternal transfer and protection role in zebrafish offspring following vaccination of the brood stock with a live attenuated Vibrio anguillarum vaccine. Aquaculture Research 2015, accepted.
3 Tongcheng Dai, Na Li, Lu Liu, Qin Liu*, Yuanxing Zhang. AMP-conjugated quantum dots: low immunotoxicity both in vitro and in vivo. Nanoscale Research Letters 2015, accepted.
4 Xiaolong Wang,·Menghao Cai*,·Lei Shi, Qi Wang, Jinxiang Zhu, Jinjia Wang, Mian Zhou,·Xiangshan Zhou, Yuanxing Zhang. PpNrg1 is a transcriptional repressor for glucose and glycerol repression of AOX1 promoter in methylotrophic yeast Pichia pastoris. Biotechnology Letters 2015, accepted.
5 Meixia Wang, Hao He, Ke Na, Menghao Cai*, Xiangshan Zhou, Wenjie Zhao, Yuanxing Zhang. Designing novel glucose/ornithine replenishment strategies by biosynthetic and bioprocess analysis to improve fibrinolytic FGFC1 production by the marine fungus Stachybotrys longispora. Process Biochemistry 2015, accepted.
6 Xiaohong Liu, Hua Zhang, Yuan Gao, Yang Zhang, Haizhen Wu*, Yuanxing Zhang. Efficacy of chitosan oligosaccharide as aquatic adjuvant administrated with a formalin-inactivated Vibrio anguillarum vaccine. Fish & Shellfish Immunology 2015, accepted.
7 Jiao Zhou, Hao He, Xiaolong Wang, Jian Lu, Xiangshan Zhou, Menghao Cai*, Yuanxing Zhang. Optimization of nutrients for dinactin production by a marine Streptomyces sp. from High Latitude Arctic. Biotechnology and Bioprocess Engineering 2015, 20(4): 725-732.
8 Zhongyang Sun, Binbing Shen, Haizhen Wu*, Xiangyu Zhou, Qiyao Wang, Jingfan Xiao, Yuanxing Zhang. The secreted fructose 1,6-bisphosphate aldolase as a broad spectrum vaccine candidate against pathogenic bacteria in aquaculture. Fish & Shellfish Immunology 2015, 46(2): 638-647.
9 Tao Jiang, Menghao Cai*, Mengmeng Huang, Hao He, Jian Lu, Xiangshan Zhou, Yuanxing Zhang. Characterization of a thermostable raw-starch hydrolyzing ?-amylase from deep-sea thermophile Geobacillus sp. Protein Expression and Purification 2015, 114: 15-22.
10 Teng Chu, Yajun Huang, Mingyu Hou, Qiyao Wang, Jingfan Xiao, Qin Liu*, Yuanxing Zhang. In vivo programmed gene expression based on artificial quorum networks. Applied and Environmental Microbiology 2015, 81(15): 4984-4992.
11 Teng Chu, Lingyu Guan, Pengfei Shang, Qiyao Wang, Jingfan Xiao, Qin Liu*, Yuanxing Zhang. A controllable bacteria lysis system to enhance biological safety of live attenuated Vibrio anguillarum vaccine. Fish & Shellfish Immunology 2015, 45(2): 742-749. 
12 Xiaohong Liu, Haizhen Wu*, Qin Liu, Qiyao Wang, Jingfan Xiao, Yuanxing Zhang. Skin-injury zebrafish, Danio rerio, are more susceptible to Vibrio anguillarum infection. Journal of the World Aquaculture Society 2015, 46(3): 301-310. 
13 Xiaohong Liu, Haizhen Wu*, Qin Liu, Qiyao Wang, Jingfan Xiao, Xinyue Chang, Yuanxing Zhang. Profiling immune response in zebrafish intestine, skin, spleen and kidney bath-vaccinated with a live attenuated Vibrio anguillarum vaccine. Fish & Shellfish Immunology 2015, 45(2): 342-345. 
14 Teng Chu, Chunshan Ni, Lingzhi Zhang, Qiyao Wang, Jingfan Xiao, Yuanxing Zhang, Qin Liu*. A quorum sensing-based in vivo expression system and its application in multivalent bacterial vaccine. Microbial Cell Factories 2015, 14: 37.
15 Shuai Shao, Qiliang Lai, Qin Liu, Haizhen Wu, Jingfan Xiao, Zongze Shao, Qiyao Wang*, Yuanxing Zhang. Phylogenomics characterization of a highly virulent Edwardsiella strain ET080813T encoding two distinct T3SS and three T6SS gene clusters: propose a novel species as Edwardsiella anguillarum sp. nov. Systematic and Applied Microbiology 2015, 38(1): 36-47.
16 Weizheng Yang, Lixia Wang, Lingzhi Zhang, Jiangbo Qu, Qiyao Wang*, Yuanxing Zhang. An invasive and low virulent Edwardsiella tarda esrB mutant promising as live attenuated vaccine in aquaculture. Applied Microbiology and Biotechnology 2015, 99(4): 1765-1777.
17 Hua Zhang, Haizhen Wu*, Liang Gao, Ying Qiu, Jingfan Xiao, Yuanxing Zhang. Identification, expression and immunological response to bacterial challenge following vaccination of BLT1 gene from turbot, Scophthalmus maximus. Gene 2015, 557(2): 229-235.
18 Haifeng Liu, Xiangshan Zhou*, Shousheng Tian, Xianghui Hao, Jinhua You, Yuanxing Zhang* (2014) Two-step transpeptidation of the insulin precursor expressed in Pichia pastoris to insulin ester via trypsin-catalyzed cleavage and coupling. Biotechnology and Applied Biochemistry 61(4): 408-417.
19 John S. Y. Goh, Yingwei Liu, Haifeng Liu, Kah Fai Chan, Corrine Wan, Gavin Teo, Xiangshan Zhou, Fusheng Xie, Peiqing Zhang, Yuanxing Zhang*, Zhiwei Song* (2014) Highly sialylated recombinant human erythropoietin production in large-scale perfusion bioreactor utilizing CHO-gmt4 (JW152) with restored GnT I function. Biotechnology Journal 9(1): 100-109.
20 Pham Ngoc Tu, Yamin Wang, Menghao Cai, Xiangshan Zhou*, Yuanxing Zhang* (2014) Co-expressions of protein tyrosine kinases EGFR-2 and PDGFRβ with protein tyrosine phosphatase 1B in Pichia pastoris. Journal of Microbiology and Biotechnology 24(2): 152-159.
21 Yingwei Liu, Xiangshan Zhou, Zhiwei Song*, Yuanxing Zhang* (2014) Sodium butyrate enhances the acidic isoform content of recombinant human erythropoietin produced by Chinese hamster ovary cells. Biotechnology Letters 36(5): 907-911.
22 Lili Sheng, Yuanzhi Lv, Qin Liu, Qiyao Wang*, Yuanxing Zhang* (2013) Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Veterinary Microbiology 162(2-4): 652-662.
23 Haifeng Liu, Xiangshan Zhou*, Fusheng Xie, Jinhua You, Yuanxing Zhang* (2013) An efficient trypsin digestion strategy for improving desB30 productivity from recombinant human insulin precursor fusion protein. Process Biochemistry 48(5-6): 965-971.
24 Dahai Yang, Qin Liu*, Mingjun Yang, Haizhen Wu, Qiyao Wang, Jingfan Xiao, Yuanxing Zhang* (2012) RNA-seq liver transcriptome analysis reveals an activated MHC-I pathway and an inhibited MHC-II pathway at the early stage of vaccine immunization in zebrafish. BMC Genomics 13: 319.
25 Menghao Cai, Xiangshan Zhou*, Jian Lu, Weimin Fan, Jiushun Zhou, Chuanpeng Niu, Li Kang, Xunqian Sun, Yuanxing Zhang* (2012) An integrated control strategy for the fermentation of the marine-derived fungus Aspergillus glaucus for the production of anti-cancer polyketide. Marine Biotechnology 14(6): 665-671.
26 Ming Liu, Shuang Wang, Tingguang Sun, Jiguo Su, Yuanxing Zhang*, Junjie Yue*, Zhiwei Sun* (2012) Insight into the structure, dynamics and the unfolding property of amylosucrases: Implications of rational engineering on thermostability. PLoS One 7(7): e40441.
27 Huan Liu, Dan Gu, Xiaodan Cao, Qin Liu, Qiyao Wang*, Yuanxing Zhang* (2012) Characterization of a new quorum sensing regulator luxT and its roles in the extracellular protease production, motility, and virulence in fish pathogen Vibrio alginolyticus. Archives of Microbiology 194(6): 439-452.
28 Menghao Cai, Xiangshan Zhou*, Jian Lu, Weimin Fan, Chuanpeng Niu, Jiushun Zhou, Xueqian Sun, Li Kang, Yuanxing Zhang* (2011) Enhancing aspergiolide A production from a shear-sensitive and easy-foaming marine-derived filamentous fungus Aspergillus glaucus by oxygen carrier addition and impeller combination in a bioreactor. Bioresource Technology 102(3): 3584-3586.
29 Menghao Cai, Xueqian Sun, Xiangshan Zhou*, Yuanxing Zhang* (2012) Roles of cobalt in biosynthesis stimulation of a cytotoxic compound from marine-derived Aspergillus glaucus. Process Biochemistry 47(12): 2267-2274.
30 Xiaodan Cao, Qiyao Wang, Qin Liu*, Haopeng Rui, Huan Liu, Yuanxing Zhang* (2011) Identification of a luxO-regulated extracellular protein Pep and its roles in motility in Vibrio alginolyticus. Microbial Pathogenesis 50(2):123-131.
31 Lu Cheng, Xiangming Sun, Xiaoping Yi, Yuanxing Zhang* (2011) Transient gene expression of a mouse homolog of Fc?-Fc? fusion protein for anti-allergic function assay. Process Biochemistry 46(4): 858-863.
32 Xin Wang, Qiyao Wang*, Minjun Yang, Jingfan Xiao, Qin Liu, Haizhen Wu, Yuanxing Zhang* (2011) QseBC controls flagellar motility, fimbrial hemagglutination and intracellular virulence in fish pathogen Edwardsiella tarda. Fish & Shellfish Immunology 30(3): 944-953.
33 Honghong He, Qiyao Wang, Lili Sheng, Qin Liu*, Yuanxing Zhang* (2011) Functional characterization of Vibrio alginolyticus twin-arginine translocation system: its roles in stress adaptation and virulence towards fish. Current Microbiology 62(4): 1193-1199.
34 Yanhua Pan, Wei Wei, Hongyan Xu, Qiyao Wang, Qin Liu, Haizhen Wu*, Yuanxing Zhang* (2011) Differential gene expression in Japanese flounder (Paralichthys olivaceus) induced by live attenuated Vibrio anguillarum. Aquaculture Research 42(7):1042-1049.
35 Huan Liu, Qiyao Wang*, Qin Liu, Xiaodan Cao, Cunbin Shi, Yuanxing Zhang* (2011) Roles of Hfq in the stress adaptation and virulence in fish pathogen Vibrio alginolyticus and its potential application as a target for live attenuated vaccine. Applied Microbiology and Biotechnology 91(2):353-364.
36 Lu Cheng, Xiangming Sun, Xiaoping Yi, Yuanxing Zhang* (2011) Large-scale plasmid preparation for transient gene expression. Biotechnology Letters 33(8): 1559-1564.
37 Yan Zhao, Qin Liu*, Xiuhua Wang, Lingyun Zhou, Qiyao Wang, Yuanxing Zhang* (2011) Surface display of Aeromonas hydrophila GAPDH in attenuated Vibrio anguillarum to develop a novel multivalent vector vaccine. Marine Biotechnology 13(5): 963-970.
38 Jingfan Xiao, Tao Chen, Qiyao Wang*, Qin Liu, Xin Wang, Yuanzhi Lv, Haizhen Wu, Yuanxing Zhang* (2011) Search for live attenuated vaccine candidate against edwardsiellosis by mutating virulence-related genes of fish pathogen Edwardsiella tarda. Letters in Applied Microbiology 53(4): 430-437.
39 Xiaonuo Teng, Xiaoping Yi, Xiangming Sun, Yuanxing Zhang* (2011) Modeling and application of controlled-fed perfusion culture of CHO cells in a bioreactor. Chemical and Biochemical Engineering Quarterly 25(3): 385-394.
40 Lili Xu, Qiyao Wang, Jingfan Xiao, Qin Liu*, Xin Wang, Tao Chen, Yuanxing Zhang* (2010) Characterization of Edwardsiella tarda waaL: roles in lipopolysaccharide biosynthesis, stress adaptation, and virulence toward fish. Archives of Microbiology 192(12):1039-1047.
41 Wei Wei, Haizhen Wu*, Hongyan Xu, Teng Chu, Changwen Wu, Kangmei Chang, Yuanxing Zhang* (2010) Cloning and molecular characterization of lycC1INH genes in large yellow croaker (Pseudosciaena crocea). Fish & Shellfish Immunology 28(1):221-227.
42 Xueqian Sun, Xiangshan Zhou*, Menghao Cai, Jiushun Zhou, Yuanxing Zhang* (2010) Significant stimulation of o-phthalic acid in biosynthesis of aspergiolide A by a marine fungus Aspergillus glaucus. Bioresource Technology 101(10):3609-3616.
43 Lingyun Zhou, Xiuhua Wang, Qin Liu*, Qiyao Wang, Yan Zhao, Yuanxing Zhang* (2010) A novel multivalent vaccine based on secretary antigen-delivery induces protective immunity against Vibrio anguillarum and Aeromonas hydrophila. Journal of Biotechnology 146(1-2): 25-30.
44 Haopeng Rui, Jennifer M. Ritchie, Roderick T. Bronson, John J. Mekalanos, Yuanxing Zhang, Matthew K. Waldor* (2010) Reactogenicity of live-attenuated Vibrio cholerae vaccines is dependent on flagellins. Proceedings of the National Academy of Sciences of the United States of America 107(9):4359-4364.
45 Xiaodan Cao, Qiyao Wang, Qin Liu*, Huan Liu, Honghong He, Yuanxing Zhang* (2010) Vibrio alginolyticus MviN is a LuxO-regulated protein and affects cytotoxicity toward EPC cell. Journal of Microbiology and Biotechnology 20(2): 271-280.
46 Xiao Zhang, Wei Wei, Haizhen Wu*, Hongyan Xu, Kangmei Chang, Yuanxing Zhang* (2010) Gene cloning and characterization of ferritin H and M subunits from large yellow croaker (Pseudosciaena crocea). Fish & Shellfish Immunology 28(5-6):735-742.
47 Menghao Cai, Xiangshan Zhou*, Jiushun Zhou, Chuanpeng Niu, Xueqian Sun, Yuanxing Zhang* (2010) Efficient strategy for enhancing aspergiolide A production by citrate feedings and its effects on sexual development and growth of marine-derived fungus Aspergillus glaucus. Bioresource Technology 101(15):6059-6068.
48 Cuihong Du, Xiaoping Yi, Yuanxing Zhang* (2010) Expression and purification of soluble recombinant human endostatin in Escherichia coli. Biotechnology and Bioprocess Engineering 15(2):229-235.
49 Ping Zhang, Wenwen Zhang, Xiangshan Zhou*, Peng Bai, James M. Cregg, Yuanxing Zhang* (2010) Catabolite repression of Aox in Pichia pastoris is dependent on hexose transporter PpHxt1 and pexophagy. Applied and Environmental Microbiology 76(18): 6108-6118.
50 Suxia Li, Haizhen Wu, Jian Zhao, Ling Ou, Yuanxing Zhang* (2010) A biochemistry and molecular biology experiment and evaluation system for biotechnology specialty students. Biochemistry and Molecular Biology Education 38(4): 271-275.
51 Xin Wang, Qiyao Wang, Jingfan Xiao, Qin Liu*, Haizhen Wu, Yuanxing Zhang* (2010) Hemolysin EthA in Edwardsiella tarda is essential for fish invasion in vivo and in vitro and regulated by two-component system EsrA-EsrB and nucleoid protein HhaEt. Fish and Shellfish Immunology 29(6):1082-1091.
52 Qiyao Wang, Minjun Yang, Jingfan Xiao, Haizhen Wu, Xin Wang, Yuanzhi Lv, Lili Xu, Huajun Zheng, Shengyue Wang, Guoping Zhao, Qin Liu*, Yuanxing Zhang* (2009) Genome sequence of the versatile fish pathogen Edwardsiella tarda provides insights into its adaptation to broad host ranges and intracellular niches. PLoS One 4(10): e7646.
53 Jingfan Xiao, Qiyao Wang, Qin Liu, Xin Wang, Huan Liu, Yuanxing Zhang* (2009) Isolation and identification of fish pathogen Edwardsiella tarda from mariculture in China. Aquaculture Research 40(1):13-17.
54 Menghao Cai, Xiangshan Zhou*, Xueqian Sun, Kejing Tao, Yuanxing Zhang* (2009) Statistical optimization of medium composition for aspergiolide A production by marine-derived fungus Aspergillus glaucus. Journal of Industrial Microbiology & Biotechnology 36(3):381-389.
55 Jingfan Xiao, Qiyao Wang*, Qin Liu, Lili Xu, Xin Wang, Haizhen Wu, Yuanxing Zhang* (2009) Characterization of Edwardsiella tarda rpoS: its roles in controlling serum resistance, chondroitinase activity, biofilm formation, and autoinducer synthetases expression. Applied Microbiology and Biotechnology 83(1):151-160.
56 Wei Wei, Hongyan Xu*, Qiyao Wang, Xiao Zhang, Kangmei Chang, Changwen Wu, Yuanxing Zhang* (2009) Identification of differentially expressed genes in large yellow croaker (Pseudosciaena crocea) induced by attenuated live Vibrio anguillarum. Aquaculture 291(1-2):124-129.
57 Xueqian Sun, Xiangshan Zhou*, Menghao Cai, Kejing Tao, Yuanxing Zhang* (2009) Identified biosynthetic pathway of aspergiolide A and a novel strategy to increase its production in a marine-derived fungus Aspergillus glaucus by feeding of biosynthetic precursors and inhibitors simultaneously. Bioresource Technology 100(18):4244-4251.
58 Haopeng Rui, Qin Liu, Qiyao Wang, Yue Ma, Huan Liu, Cunbin Shi, Yuanxing Zhang* (2009) Role of alkaline serine protease Asp in Vibrio alginolyticus virulence and regulation of its expression by LuxO-LuxR regulatory system. Journal of Microbiology and Biotechnology 19(5): 431-438.
59 Wei Wei, Haizhen Wu, Hongyan Xu*, Tao Xu, Xiao Zhang, Kangmei Chang, Yuanxing Zhang* (2009) Cloning and molecular characterization of two complement Bf/C2 genes in large yellow croaker (Pseudosciaena crocea). Fish & Shellfish Immunology 27(2):285~295.
60 Xin Wang, Qiyao Wang*, Jingfan Xiao, Qin Liu, Haizhen Wu, Lili Xu, Yuanxing Zhang* (2009) Edwardsiella tarda T6SS component evpP is regulated by esrB and iron, and plays essential roles in the invasion of fish. Fish & Shellfish Immunology 27(3): 469~477.
61 Yuanxing Zhang (2009) Approaches to optimizing animal cell culture process: Metabolic regulation and protein expression improvement. Advances in Biochemical Engineering/ Biotechnology 113: 177-215.
62 Qiyao Wang, Zhao Yang, Qin Liu*, Yuanxing Zhang* (2009) Surface-exposed expression of Edwardsiella tarda EseB in live attenuated Vibrio anguillarum based on novel surface display systems. Aquaculture Research 40(13):1459-1467.

Corresponding Address:

School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. 
Phone and fax: 021-64253025
Email: yxzhang@ecust.edu.cn