Yingping ZHUANG

Author： CreatedTime：2018-09-05 21:09:00

Yingping ZHUANG , Female，Ph.D., Professor . She was born in 1962 and graduated in 1984 of ECUST. Vice Director of College of Biotechnology, Vice Director of National Research Engineering Research Center for Biotechnology (Shanghai), Recent years, she worked in group of bioprocess engineer of SLBRE and studied in optimization and scale-up of fermentation. They formed a new method of multi-scale optimization on fermentation process , invented a new equipment used to optimization of fermentation. With this method and equipment, they had breakthrough in productivity of penicillin, erythromycin, aureomycin, guanosine, inosine，recomdinant human albumin, phytase ,etc. She was awarded 2^nd Advancement of Science and Technology (AST) by Chinese central government in 2002, 2004 and 2011（Order: 3）and 1^st AST from Ministry of Shanghai Municipality in 2001,2003 and 2008(Order: 3) .She was good at association analysis of data in fermentation process, regulation of second metabolism and scale-up of industrial bio-process. In recent years she has published more than 100 papers .

Tel：021-64251257，Email: ypzhuang@ecust.edu.cn

The studying item :

National 973 project: The method of scale-up industrial bio-process.
National 11th five 863 project: Research of the process of erythromycin, cephalosporin
National 10th five year plan: The optimization of meilingmycin’s fermentation
National 863 project: Research of plat of bio-pharmaceutical
Shanghai ministry development foundation: The optimization of industrial fermentation.

Education:

BS (ECUST) 1984;
MS (ECUST) 1997;
Ph.D.(ECUST) 2006

Honour:

? Fermentation process optimization and scale-up technique based on multi-parameters correlation and the study on the bioreactor equipments, awarded 1st AST from Ministry of Shanghai Municipality, 2001 (Order: 3)
? Fermentation process optimization and scale-up technique based on multi-parameters correlation, awarded 2nd Advancement of Science and Technology (AST) by Chinese central government, 2002, (Order: 3)
? A multi-scale study on fermentation process and optimization of a novel food additive, guanosine production, awarded 1st AST from Ministry of Shanghai Municipality, 2003 (Order: 3)
? Make high specific active gene of phytase and production of phytase resisting high temperature, awarded 1st AST from Ministry of Shanghai Municipality, 2003 (Order: 7)
? The Key technology for the production of “I+G”, awarded 2nd Advancement of Science and Technology (AST) by Chinese central government, 2004, (Order: 4)
? An intergrated novel technology for industral erythromycin production, guanosine production, awarded 1st AST from Ministry of Shanghai Municipality, 2008(Order: 3)
? A novel Industrial fermentation technology based on the integration of microbial physiology and process information treatment, awarded 2nd Advancement of Science and Technology (AST) by Chinese central government, 2011, (Order: 3)

Publication:

1. Yong Chen, Zejiang Wang, Ju Chu*, Beili Xi, Yingping Zhuang. The glucose RQ-feedback control leading to improved erythromycin production by a recombinant strain Saccharopolyspora erythraea ZL1004 and its scale-up to 372-m(3) fermenter. Bioprocess & Biosystems Engineering. 2015, 38(1):105-112, DOI: 10.1007/s00449-014-1248-8
2. Hongzhong Lu，Chao Li，Wenjun Tang，Zejian Wang，Jianye Xia，Siliang Zhang，Yingping Zhuang，Ju Chu，Henk Noorman，Dependence of fungal characteristics on seed morphology and shear stress in bioreactor. Bioprocess & Biosystems Engineering. 2015, 38(5):917-928, DOI: 10.1007/s00449-014-1337-8
3. Fei Shi, Jun Tan, Ju Chu, Yonghong Wang, Yingping Zhuang, Siliang Zhang. A qualitative and quantitative high-throughput assay for screening of gluconate high-yield strains by Aspergillus niger. Journal of Microbiologcal Methods. 109:134-139, DOI: 10.1016/ j.mimet.2014.12.004
4. Guan Wang, Ju Chu, Henk Noorman, Jianye Xia, Wenjun Tang, Yingping Zhuang, Siliang Zhang. Prelude to rational scale-up of penicillin production: a scale-down study. Applied Microbiology and Biotechnolog. 2014,   DOI:10.1007/s00253-013-5497-2
5. Minghui Xie, Jianye Xia*, Zhen Zhou, Ju Chu, Yingping Zhuang, Siliang Zhang, Flow Pattern, Mixing, Gas Hold-Up and Mass Transfer Coefficient of Triple-Impeller Configurations in Stirred Tank Bioreactors. Industrial & Engineering Chemistry Research. 2014, 53(14): 5941-5953, DOI: 10.1021/ie400831s
6. Minghui Xie, Jianye Xia*, Zhen Zhou,Zhou Guozhong, Ju Chu, Yingping Zhuang, Siliang Zhang, Henk Noorman, Power consumption, local and average volumetric mass transfer coefficient in multiple-impeller stirred bioreactors for xanthan gum solutions. Chemical Engineering Science. 2014, 106:144-`56. DOI: 10.1016/j.ces.2013.10.032
7. Lan Li, Zejian Wang*, Xuejun Chen, Ju Chu, Yingping Zhuang, Siliang Zhang, Optimization of polyhydroxyalkanoates fermentations with on-line capacitance measurement. Bioresource Technology. 2014, 156:216-221. DOI: 10.1016/j.biortech.2014.01.042
8. Lei Deng, Bingyang Shi, Yingping Zhuang, Ju Chu, Xiaolin Shi, Siliang Zhang, Meijin Guo*, Performance and Mechanism of Neuroleukin in the Growth and Survival of Sertoli Cell-Induced Neurons in a Coculture System. Cell Transplantation. 2014, 23(3): 381-394, DOI: 10.3727/096368913X663578
9. Xiwei Tian, Yonghong Wang*, Ju Chu*, Yingping Zhuang, Siliang Zhang,. Oxygen transfer efficiency and environmental osmolarity response to neutralizing agents on L-lactic acid production efficiency by Lactobacillus paracasei. Process Biochemistry. 2014,49(12): 2049-2054, DOI: 10.1016/j.procbio.2014.06.014
10. Yongsheng Nie, Mingzhi Huang*, Junjie Lu, Jiangchao Qian, Weilu Lin, Ju Chu, Yingping Zhuang, Siliang Zhang. Impacts of high beta-galactosidase expression on central metabolism of recombinant Pichia pastoris GS115 using glucose as sole carbon source via C-13 metabolic flux analysis. Journal of Biotechnology. 2014, 187:124-134, DOI: 10.1016/j.jbiotec.2014.07.011
11. Xiaoqing Hu, Ju Chu, Siliang Zhang, Yingping Zhuang, Xin Wu, Huaxin Chen, Zhongyuan Lv, Zhongyi Yuan. An alkaline pH control strategy for methionine adenosyltransferase production in Pichia pastoris fermentation. Biotechnology & Bioprocess Engineering. 2014, 19(5): 900-907, DOI: 10.1007/s12257-014-0046-0
12. Xiwei Tian, Yonghong Wang*, Ju Chu*, Yingping Zhuang, Siliang Zhang,. L-Lactic acid production benefits from reduction of environmental osmotic stress through neutralizing agent combination. Bioprocess & Biosystems Engineering. 2014, 37(9): 1917-1923, DOI: 10.1007/s00449-014-1166-9
13. Taicheng Zhu, Haifeng Hang, Ju Chu, Yingping Zhuang, Siliang Zhang, Meijin Guo. Transcriptional investigation of the effect of mixed feeding to identify the main cellular stresses on recombinant Pichia pastoris. Journal of Industrial Microbiology & Biotechnology. 2013, 40(2) : 183-189   DOI: 10.1007/s10295-012-1225-7
14. Ju Chu, Jiangchao Qian, Yingping Zhuang, Siliang Zhang. Progress in the research of S-adenosyl-L-methionine production. Applied Microbiology Biotechnology. 2013, 97(1):41-49   DOI: 10.1007/s00253-012-4536-8
15. Yong Chen,Mingzhi Huang,Zejian Wang,Ju Chu,Yingping Zhuang,Siliang Zhang, Controlling the feed rate of glucose and propanol for the enhancement of erythromycin production and exploration of propanol metabolism fate by quantitative metabolic flux analysis. Bioprocess and Biosystems Engineering. 2013, 36(10): 1445-1453
16. Jun Tan,Ju Chu,Yuyou Hao,Yuanxin Guo,Yingping Zhuang,Siliang Zhang, High-Throughput System for Screening of Cephalosporin C High-Yield Strain by 48-Deep-Well Microtiter Plates. Applied Biochemistry and Biotechnology. 2013, 169(5): 1683-1695
17. Chao Li, Jianye Xia*, Ju Chu, Yonghong Wang*, Yingping Zhuang,; Siliang Zhang, CFD analysis of the turbulent flow in baffled shake flasks. Biochemical Eneineering Journal. 2013, 70:140-150
18. Yong Chen, Zejian Wang, Ju Chu, Yingping Zhuang, Siliang Zhang, Yu Xiaoguan, Significant decrease of broth viscosity and glucose consumption in erythromycin fermentation by dynamic regulation of ammonium sulfate and phosphate. Bioresource Technology. 2013,134:173-179.
19. Xiang Zou,Wanjun Li,Wei Zeng,Haifeng Hang,Ju Chu,Yingping Zhuang,Siliang Zhang, Biochemical Parameters of Saccharopolyspora Erythraea during Feeding Ammonium Sulphate in Erythromycin Biosynthesis Phase. Applied Biochemistry & Microbiology. 2013,49(2): 169-175   DOI: 10.1134/S0003683813020166
20. Xiang Zou, Wanjun Li, Wei Zeng, Hangfeng Hang, Ju Chu, Yingping Zhuang, Siliang Zhang. Biochemical Parameters of Saccharopolyspora Erythraea during Feeding Ammonium Sulphate in Erythromycin Biosynthesis Phase. Applied Biochemistry & Microbiology. 2013, 49(2):169-175
21. Xiaoqing Hu, Ju Chu. Siliang Zhang. Yingping Zhuang, Comparative performance of S-adenosyl-L-methionine biosynthesis and degradation in Pichia pastoris using different promoters and novel consumption inhibitors. 2013, Enzyme & Microbial Technology. 55:94-99. DOI: 10.1016/j.enzmictec.2013.09.003
22.Xiang Zou, Jian-ye Xia, Ju Chu, Ying-ping Zhuang, Si-liang Zhang, Real-time fluid dynamics investigation and physiological response for erythromycin fermentation scale-up from 50 L to 132 m(3) fermenter. Bioprocess & Biosystem Engineering, 2012, 35:789–800
23. Yue Hugh Guan, Remco N. A. M van den Heuvel, Ying-Ping Zhuang, Visualisation of J-type counter-current chromatography: A route to understand hydrodynamic phase distribution and retention. Journal of Chromatography A . 2012, 1239:10-21
24. Lan Yu, Nan Cao, Long Wang, Ciying Xiao, Meijin Guo, Ju Chu, Yingping Zhuang, Siliang Zhang, Oxytetracycline biosynthesis improvement in Streptomyces rimosus following duplication of minimal PKS genes. Enzyme & Microbial Technology, 2012, 50(6-7): 318-324
25. Ze-Jian Wang, Ping Wang, Yu-Wei Liu, Yi-Ming Zhang, Ju Chu, Ming-zhi Huang, Yingping Zhuang, Siliang Zhang, Metabolic flux analysis of the central carbon metabolism of the industrial vitamin B-12 producing strain Pseudomonas denitrificans using C-13-labeled glucose. Journal of the Taiwan Institute of Chemical Engineers. 2012, 43(2): 181-187
26. Dan Wu, Ju Chu, Yu-You Hao, Yong-Hong Wang, Ying-Ping Zhuang, Si-Liang Zhang, Incomplete protein disulphide bond conformation and decreased protein expression result from high cell growth during heterologous protein expression in Pichia pastoris. Journal of Biotechnology. 2012,157(1):107-112
27. Yiming Yang, Jianye Xia, Jianhua Li ,Ju Chu, Liang Li, YonghongWang, Yingping Zhuang ,Siliang Zhang, A novel impeller configuration to improve fungal physiology performance and energy conservation for cephalosporin C production, Journal of Biotechnology. 2012, 161(3) : 250-256   DOI: 10.1016/j.jbiotec.2012.07.007
28. Jun Tan, Ju Chu, Wenjuan Shi, Cheng Lin, Yuanxin Guo, Yingping Zhuang, Siliang Zhang, Tadayuki I manaka. High-throughput screening strategy used for enhanced production of pigment by Monascus purpureus D39-4. Food Science and Biotechnology. 2012, 21(6): 1603-1610   DOI: 10.1007/s10068-012-0213-z
29. Xiang Zou, Wan-jun Li, Wei Zeng, Ju Chu, Ying-ping Zhuang, Si-liang Zhang. An assessment of seed quality on erythromycin production by recombinant Saccharopolyspora erythraea strain . Bioresource Technology , 2011,102(3): 3360-3365
30. Xu Li , Yonghong, Siliang, Ju Chu , Ming Zhang , Mingzhi Huang , Yingping Zhuang. Effects of light/dark cycle, mixing pattern and partial pressure of H-2 on biohydrogen production by Rhodobacter sphaeroides ZX-5. Bioresource Technology , 2011,102(2): 1142-1148
31. Taicheng Zhu , Meijin Guo , Yingping Zhuang , Ju Chu , Siliang Zhang. Understanding the effect of foreign gene dosage on the physiology of Pichia pastoris by transcriptional analysis of key genes. Applied Microbiology & Biotechnology. 2011, 89(4): 1127-1135
32. Xiulin Qin, Jiangchao Qian*, Gaofeng Yao, Yingping Zhuang, Siliang Zhang, and Ju Chu, GAP Promoter Library for Fine-Tuning of Gene Expression in Pichia pastoris. Applied and Environmental Microbiology, 2011, 77(11):3600-3608
33 .Suwen Hu, Lei Deng, Huamao Wang, Yingping Zhuang, Ju Chu, Siliang Zhang, Zhonghai Li, Meijin Guo. Bioprocess development for the production of mouse-human chimeric anti- epidermal growth factor receptor vIII antibody C12 by suspension culture of recombinant Chinese hamster ovary cells. Cytotechnology. 2011,63(3): 247-258
34. Xu Li, Huan Shi,, Yonghong Wang, , Siliang Zhang, Ju Chu, Ming Zhang, Mingzhi Huang, Yingping Zhuang, , Effects of vitamins (nicotinic acid, vitamin B(1) and biotin) on phototrophic hydrogen production by Rhodobacter sphaeroides ZX-5. International Journal of Hydrogen energy, .2011,36(16) : 9620-9625
35. Dan Wu, Ju Chu, Yu-You Hao, Yong-Hong Wang, Ying-Ping Zhuang, Si-Liang Zhang, High efficient production of recombinant human consensus interferon mutant in high cell density culture of Pichia pastoris using two phases methanol control. Process Biochemistry. 2011,46(8): 1663-1669
36. Zhenyu Tang, Ciying Xiao, Yingping Zhuang, Ju Chu, Siliang Zhang, Herron, Paul R., Hunter, Iain S., Meijin Guo, Improved oxytetracycline production in Streptomyces rimosus M4018 by metabolic engineering of the G6PDH gene in the pentose phosphate pathway, Enzyme & Microbial Technology, 2011, 49(1): 17-24
37. Jian-Guang Liang, Xiao-He Chu, , Zhi-Qiang Xiong, Ju Chu, Yong-Hong Wang, Ying-Ping Zhuang, Si-Liang Zhang, Oxygen uptake rate regulation during cell growth phase for improving avermectin B(1a) batch fermentation on a pilot scale (2 m(3)). World Journal of Microbiology & Biotechnology, 2011, 27(11) : 2639-2644
38. Dan Wu, Ju Chu, Yu-You Hao, Yong-Hong Wang, Ying-Ping Zhuang, Si-Liang Zhang, Incomplete protein disulphide bond conformation and decreased protein expression result from high cell growth during heterologous protein expression in Pichia pastoris. J. Biotechnology, 2011. 157(1):107-112
39. Yifeng Lu ,Yonghong Wang, Guoqian Xu , Ju Chu, Yingping Zhuang , Siliang Zhang,Influence of High Solid Concentration on Enzymatic Hydrolysis and Fermentation of Steam-Exploded Corn Stover Biomass. Appl Biochem Biotechnol, 2010, 160:360–369
40. Jie Chen, Siliang Zhang, Ju Chu, Yingping Zhuang, Jiali Luo and Hua Bai, Ethanol evolution rate: a new parameter to determine the feeding rate for the production of avermectins by Streptomyces avermitilis, Biotechnology Letters, 26:109-113, 2004
41. Ju Chu ,Wenze Niu, Siliang Zhang, Yingping Zhuang, Yourong Li ,Effect of metal ions on the binding of gentamicin to the peptidoglycan of Micromonospora echinospora, Process Biochemistry, 39(2004)1145-1150
42. XinHao Ye, Ju Chu, YingPing Zhuang, SiLiang Zhang, Multi-scale methodology:A key to deciphering systems biology，Frontiers in Bioscience, 2005，10:961-965
43. Ju Chu, Siliang Zhang, Yingping Zhuang, Yongbo Song, Xianpeng Cai, Correlation between key enzyme activities in the inosine synthetic pathway and inosine production. Process Biochemistry ,40:891-894, 2005
44. Dan Wu, Dong Ma, Yu-You Hao, Ju Chu, Yong-Hong, Wang,Ying-Ping Zhuang , Si-Liang Zhang, Incomplete formation of intramolecular disulfide bond triggers degradation and aggregation of human consensus interferon-α mutant by Pichia pastoris. Appl Microbiol Biotechnol , 2010, 85:1759–1767
45. Ze-Jian Wang, Hui-Yuan Wang , Yong-Liang Li, Ju Chu , Ming-Zhi Huang, Ying-Ping Zhuang, Si-Liang Zhang, Improved vitamin B12 production by step-wise reduction of oxygen uptake rate under dissolved oxygen limiting level during fermentation process. Bioresource Technology , 2010,101: 2845-2852
46. Yifeng Lu ,Yonghong Wang, Guoqian Xu , Ju Chu, Yingping Zhuang , Siliang Zhang,Influence of High Solid Concentration on Enzymatic Hydrolysis and Fermentation of Steam-Exploded Corn Stover Biomass. Appl Biochem Biotechnol, 2010, 160:360–369
47. Yong-Hong Wang, Chun-Hai Wu, Ju Chu, Yu-You Hao, Ying-Ping Zhuang , Si-Liang Zhang, Regulation of branched-chain amino acid catabolism: glucose limitation enhances the component of isovalerylspiramycin for the bitespiramycin production. Bioprocess & Biosystem Engineering, 2010, 33:257–265
48. Dan Wu, Dong Ma, Yu-You Hao, Ju Chu, Yong-Hong Wang, Ying-Ping Zhuang , Si-Liang Zhang, Incomplete formation of intramolecular disulfide bond triggers degradation and aggregation of human consensus interferon-α mutant by Pichia pastoris. Appl Microbiol Biotechnol , 2010, 85:1759–1767
49. Siliang Zhang, Ju Chu, Yingping Zhuang, A Multi-Scale Study of Industrial Fermentation Processes and Their Optimization, Adv Biochem Engin/Biotechnol , 87: 97–150, 2004
50. Zhang SL, Cao XJ, Chu J, Qian JC, Zhuang YP ,Bioreactors and Bioseparation, Biotechnology in China: Chemicals, Energy & Envronment. Advances in Biochemical Engineering-Biotechnology.2010, 122: 105-150
51. Xiang Zou , Hai-feng Hang , Ju Chu, Ying-ping Zhuang, Si-liang Zhang, Oxygen uptake rate optimization with nitrogen regulation for erythromycin production and scale-up from 50 L to 372 m3 scale. Bioresource Technology. 2009, 100 :1406–1412
52. Jin Zhou 1, Yong-Hong Wang, Ju Chu, Ling-Zhi Luo, Ying-Ping Zhuang, Si-Liang Zhang,Optimization of cellulase mixture for efficient hydrolysis of steam-exploded corn stover by statistically designed experiments.Bioresource Technology ,2009,100 :819–825
53. Zhi-Qiang Xiong, Mei-Jin Guo , Yuan-Xin Guo, Ju Chu, Ying-Ping Zhuang, Si-Liang Zhang，Efficient extraction of intracellular reduced glutathione from fermentation broth of Saccharomyces cerevisiae by ethanol，Bioresource Technology，2009，100 :1011–1014
54. Guo-Qian Xu, Ju Chu, Ying-Ping Zhuang, Yong-Hong Wang, Si-Liang Zhang, Effects of vitamins on the lactic acid biosynthesis of Lactobacillus paracasei NERCB 0401.Biochemical Engineering Journal. 2008, 38(2):189-197
55. Jin Zhou, Ju Chu, Yong-Hong Wang, Hui Wang, Ying-Ping Zhuang, Si-Liang Zhang, Purification and bioactivity of exendin-4, a peptide analogue of GLP-1, expressed in Pichia pastoris. Biotechnology Letter. 2008, 30:651–656
56. Yun Chen, Wei Deng, Jiequn Wu, Jiangchao Qian, Ju Chu, Yingping Zhuang, Siliang Zhang and Wen Liu. Genetic Modulation of the Overexpression of Tailoring Genes eryK and eryG Leading to the Improvement of Erythromycin A Purity and Production in Fermentation of Saccharopolyspora erythraea. Applied and Environmental Microbiology. 2008, 74(6): 1820-1828.（4.004
57. Zhao J, Wang YH, Chu J, Zhang SL, Zhuang YP and Yuan ZY. Statistical optimization of medium for the production of pyruvate oxidase by the recombinant Escherichia coli. Journai of Industrial Microbiology & Biotechnology, 2008, 35: 257–262.（1.681）
58. Peng Yin, Yong-Hong Wang, Si-Liang Zhang, Ju Chu, Ying-Ping Zhuang, Mei-Long Wang, Jin Zhou， Isolation of soluble proteins from an industrial strain Streptomyces avermitilis in complex culture medium for two-dimensional gel electrophoresis. Journal of Microbiological Methods. 2008,73 (2) 105–110（2.153）
59. Feng Lei, Guo Meijin, Zhang Shuxiang, Chu Ju, Zhuang Yingping, Zhang Siliang, Improvement in the suspension-culture production of recombinant adeno-associated virus-LacZ in HEK-293 cells using polyethyleneimine –DNA complexes in combination with hypothermic treatment. Biotechnology and Applied Biochemistry, 2008, 50: 121-132
60. Xiong ZQ, Guo MJ, Guo YX, Chu J, Zhuang YP and Zhang SL. Real-time viable-cell mass monitoring in high-cell-density fed-batch glutathione fermentation by Saccharomyces cerevisiae T65 in industrial complex medium. Journal of Bioscience and Bioengineering, 2008, 105(4): 409-413.（1.782）
61. Zhou J, Wang YH, Chu J, Zhuang YP, Zhang SL and Yin P. Identification and purification of the main components of cellulases from a mutant strain of Trichoderma viride T 100-14. Bioresource Technology, 2008, 99: 6826–6833.（3.103）
62. Kun-Tai Li, Dong-Hong Liu, Ju Chu, Yong-Hong Wang, Ying-Ping Zhuang, Si-Liang Zhang. An effective and simplified pH-stat control strategy for the industrial fermentation of vitamin B12 by Pseudomonas denitrificans. Bioprocess and Biosystems Engineering, 2008, 31（6）：606-610
63. Kun-Tai Li, Dong-Hong Liu, Ying-Ping Zhuang, Yong-Hong Wang, Ju Chu, Si-Liang Zhang. Influence of Zn2+, Co2+ and dimethylbenzimidazole on vitamin B12 biosynthesis by Pseudomonas denitrificans . World Journal of Microbiology and Biotechnology, 2008, 24（11）：2525-2530
64. Kun-Tai Li, Dong-Hong Liu, Yong-Liang Li, Ju Chu,Yong-Hong Wang, Ying-Ping Zhuang, Si-Liang Zhang. Improved large-scale production of vitamin B12 by Pseudomonas denitrificans with betaine feeding. Bioresource Technology, 2008, 99（17）：8516-8520
65. Xiao-Qing Hu, Ju Chu, Zhuo Zhang, Si-Liang Zhang, Ying-Ping Zhuang, Yong-Hong Wang, Mei-Jin Guo, Hua-Xin Chen, Zhong-Yi Yuan. Effects of different glycerol feeding strategies on S-adenosyl-L-methionine biosynthesis by PGAP-driven Pichia pastoris overexpressing methionine adenosyltransferase. Journal of Biotechnology ,2008, 137(1-4):44-49
66. Dan Wu, Yu-You Hao, Ju Chu, Ying-Ping Zhuang, Si-Liang Zhang, Inhibition of degradation and aggregation of recombinant human consensus interferon-α mutant expressed in Pichia pastoris with complex medium in bioreactor. Applied Microbiology Biotechnology. 2008, 80(6):1063–1071
67. Hai-Feng Hang, Wen Chen, Mei-Jin Guo, Ju Chu, Ying-Ping Zhuang, Si-Liang Zhang, A simple unstructured model-based control for efficient expression oe recombination porcine insulin precursor by Pichia pastoris, Korean Journal of Chemical Engineering. 2008, 25(5):1065-1069
68. Huaxin Chen, Ju Chu , Siliang Zhang, Yingping Zhuang , Jiangchao Qian, Yonghong Wang , Xiaoqing Hu, Intracellular expression of Vitreoscilla hemoglobin improves S-adenosylmethionine production in a recombinant Pichia pastoris, Appl Microbiol Biotechnol, 2007,74 (6): 1205-1212
69. Mei-jin Guo, Ying-ping Zhuang, Ju Chu, Si-liang Zhang, Ai-sheng Xiong, Ri-he Peng, and Quan-hong Yao. Production and purification of a novel thermostable phytase by Pichia pastoris FPHY34. Process Biochemistry, 2007, 42（12）：1660-1665
70. Wang Yong, Wang Yiguang, Chu Ju, Zhuang Yingping, Zhang Lixin, Zhang Siliang,Improved production of erythromycin A by expression of a heterologous gene encoding S-adenosylmethionine synthetase ,Applied Microbiology and Biotechnology,2007, 75 (4): 837-842
71. Hu Xiaoqing, Chu Ju, Zhang Siliang, Zhuang Yingping, Wang Yonghong, Zhu Shan, Zhu Zhigang, Yuan Zhongyi, A novel feeding strategy during the production phase for enhancing the enzymatic synthesis of S-adenosyl-L-methionine by methylotrophic Pichia pastoris ,Enzyme and Microbial Technology, 2007, 40 (4): 669-674
72. Hao Yuyou, Chu Ju, Wang Yonghong, Zhuang Yingping, Zhang Siliang, The inhibition of aggregation of recombinant human consensus interferon-alpha mutant during Pichia pastoris fermentation , Applied Microbiology and Biotechnology ,2007,74 (3): 578-584
73. Feng Lei, Guo Meijin, Zhang Shuxiang, Chu Ju, Zhuang Yingping, Zhang Siliang, Optimization of transfection mediated by calcium phosphate for plasmid rAAV-LacZ (recombinant adeno-associated virus-beta-galactosidase reporter gene) production in suspension-cultured HEK-293 (human embryonic kidney 293) cells , Biotechnology and Applied Biochemistry, 2007, 46: 127-135
74. Zhang SL, Ye BC, Chu J, Zhuang YP, Guo MJ, From multi-scale methodology to systems biology: to integrate strain improvement and fermentation optimization, Journal of Chemical Technology and Biotechnology, 2006,81 (5): 734-745
75. Guo-qian Xu, Ju Chu, Yong-hong Wang, Ying-ping Zhuang, Si-liang Zhang and Hua-qiong Peng. Development of a continuous cell-recycle fermentation system for production of lactic acid by Lactobacillus paracasei. Proces .Biochemistry, 41(12): 2458-2463, 2006